A5028922455- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- Microfluidic and Bio-sensing Technologies
- Force Microscopy Techniques and Applications
- RNA Interference and Gene Delivery
- Advanced Sensor and Energy Harvesting Materials
- Cellular Mechanics and Interactions
- Analytical Chemistry and Sensors
- Mechanical and Optical Resonators
- Modular Robots and Swarm Intelligence
- Advanced biosensing and bioanalysis techniques
- Electrowetting and Microfluidic Technologies
- 3D Printing in Biomedical Research
- Gold and Silver Nanoparticles Synthesis and Applications
- Image Processing Techniques and Applications
- Electrochemical sensors and biosensors
- Microbial Inactivation Methods
- CCD and CMOS Imaging Sensors
- Advanced Memory and Neural Computing
- Bacillus and Francisella bacterial research
- Neuroscience and Neuropharmacology Research
- Molecular Communication and Nanonetworks
- Orbital Angular Momentum in Optics
- Neurogenesis and neuroplasticity mechanisms
- Cancer Research and Treatments
University of California, Los Angeles
2015-2022
Modeling studies suggest that clustered structural plasticity of dendritic spines is an efficient mechanism information storage in cortical circuits. However, why new occur specific locations and how their formation relates to learning memory (L&M) remain unclear. Using vivo two-photon microscopy, we track spine dynamics retrosplenial cortex before, during, after two forms episodic-like find turnover before predicts future L&M performance, as well the localization rates clustering....
Efficient intracellular delivery of biomolecules into cells that grow in suspension is great interest for biomedical research, such as applications cancer immunotherapy. Although tremendous effort has been expended, it remains challenging existing transfer platforms to deliver materials efficiently cells. Here, we demonstrate a high-efficiency photothermal approach using sharp nanoscale metal-coated tips positioned at the edge microwells, which provide controllable membrane disruption each...
Abstract A novel manufacturing approach to fabricate liquid metal‐based, multifunctional microcapillary pipettes able provide electrodes with high electrical conductivity for high‐frequency stimulation and measurement is proposed. 4D single cell manipulation realized by applying multifrequency, multiamplitude, multiphase signals the microelectrodes near pipette tip create 3D dielectrophoretic trap 1D electrorotation, simultaneously. Functions such as trapping, patterning, transfer, rotation...
Abstract We report a simple approach to the production of carbon fiber‐based amperometric microbiosensors for selective detection hydrogen peroxide (H 2 O ), which was achieved by electrometallization fiber microelectrodes (CFMs) electrodeposition Pt nanoparticles. The Pt‐carbon hybrid sensing interface provided sensitivity 7711±587 μA ⋅ mM −1 cm −2 , limit 0.53±0.16 μM (S/N=3), linear range 0.8 μM–8.6 mM, and response time <2 sec. morphologies nanoparticle‐modified CFMs were...
PDMS thin-film transfer and enzyme microstamping enabled 3-in-1 Si/PDMS hybrid chemtrode for multi-analyte sensing chemical delivery <italic>in vivo</italic>.
A plasmonic micropillar platform with self-organized gold nanospheres is reported for the precision cell traction force measurement across a large field-of-view (FOV). Gold were implanted into tips of polymer micropillars by annealing microdisks nanosecond laser pulses. Each nanosphere physically anchored in center pillar tip and serves as strong, point-source-like light scattering each micropillar. This allows to be clearly observed precisely tracked even under low magnification objective...
We report a general method to fabricate miniaturized soft robots. For the first time, freestanding microscale robotic finger/tentacle has been realized by heterogeneous integration of material polydimethylsiloxane (PDMS), liquid metal (LM), and silicon chips. Unlike existing methods make electronics or robotics, our technique enables large-area parallel manufacturing micro robotics armed with distributed crystalline silicon, deformable microfluidic channels, flexible electrical...
We demonstrate a 3-in-1 hybrid silicon-PDMS neural probe with electrochemical sensing, directional optical stimulation, and drug delivery capabilities to manipulate record cell activities. Blue light wavelength of 450nm is coupled onto the by grating coupler delivered tip through silicon nitride waveguide. Another at couples out provides illumination near center sensing electrode. A liquid channel bonded on top using novel PDMS thin-film transfer process.
The brain is the center of nervous system controlling other organs. characterized by extreme complexity its structure and delicate texture, which makes it difficult to investigate invasive methods. advances in semiconductor manufacturing technology led development implantable microelectromechanical (MEMS)-based devices that can be implanted into with reduced risk damage tissue due their small size low stiffness. Such are now widely applied clinic research for understanding functions,...
A novel gold nanoparticle embedded plasmonic micropillar (PEM) platform for large area, massively parallel, and high-precision cell force measurement is demonstrated. Gold nanoparticles implanted into PDMS micropillars by laser pulses serve as strong point-source-like scattering centers in dark-field imaging. These mechanically anchored are physically robust chemically stable. Precision tracking of can be realized under a low numerical aperture (NA) objective lens simultaneous parallel...
We report a novel manufacturing approach to fabricate liquid metal-based, multifunctional microcapillary pipettes able provide electrodes with high electrical conductivity for frequency stimulation and measurement. Four-dimensional single cell manipulation has been realized by applying multi-frequency, multi-amplitude, multi-phase signals the microelectrodes near pipette tip create 3D dielectrophoretic trap 1D electrorotation simultaneously. Functions such as trapping, transferring,...
Technologies for transferring large-sized cargo into mammalian cells are needed to advance key applications in cell engineering. However, reliable methodologies introducing nearly completely lacking. This talk will present two new technologies, photothermal nanoblade and BLAST, that overcome the size limitation of delivery cells.
We demonstrated a photothermal delivery approach with self-aligned cell seeding, which was realized by simple fabrication of microwells and sharp tips. Calcein molecules (622 Da) were successfully delivered into cells above 80% viability.
We demonstrated a parallel nanomechanical indentation platform using quantitative phase imaging to detect changes in optical path length of reflected light from rigid and flat silicon micro-mirrors. Three-dimensional local structure deformation force distribution can potentially be mapped across large area at the same time.
The ability to monitor neurotransmitter release in freely behaving animals is key understanding neuronal processes underlying complex behaviors. Commonly used methods either offer rapid measurements of a single analyte (e.g., fast-scan cyclic voltammetry) or provide multiple measurement with insufficient temporal resolution (microdialysis). However, behaviors are controlled by networks employing neurotransmitters and neuromodulators. Efforts understand how neurons within these interact...