A5045756812- HIV Research and Treatment
- Hepatitis C virus research
- Hepatitis B Virus Studies
- HIV/AIDS Research and Interventions
- Advanced Breast Cancer Therapies
- Cancer-related Molecular Pathways
- interferon and immune responses
- HIV/AIDS drug development and treatment
- Liver Disease Diagnosis and Treatment
- Immune Cell Function and Interaction
- Adolescent Sexual and Reproductive Health
- Biochemical effects in animals
- Chronic Lymphocytic Leukemia Research
- SARS-CoV-2 and COVID-19 Research
- Vibrio bacteria research studies
- Diet, Metabolism, and Disease
- HIV-related health complications and treatments
- Telemedicine and Telehealth Implementation
- Biochemical Acid Research Studies
- Health disparities and outcomes
- Cancer Cells and Metastasis
- Global Health Care Issues
- Viral Infections and Immunology Research
- Garlic and Onion Studies
- Mobile Health and mHealth Applications
Gladstone Institutes
2025
Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol
2020-2024
Universitat Autònoma de Barcelona
2020-2024
IrsiCaixa
2020-2023
Hospital Universitari Germans Trias i Pujol
2020-2022
Institute for Health Metrics and Evaluation
2021
University of Washington
2021
University of Lisbon
2018-2020
Escola Superior de Saúde Egas Moniz
2018
In 2016, the World Health Assembly adopted resolution to eliminate viral hepatitis by 2030. This study aims provide an overview of burdens B virus (HBV) and C (HCV) in Europe their changes from 2010 2019 using estimates Global Burden Diseases, Injuries, Risk Factors Study (GBD) 2019.
Human immunodeficiency virus and acquired immune deficiency syndrome (HIV/AIDS) is still among the leading causes of disease burden mortality in sub-Saharan Africa (SSA), world not on track to meet targets set for ending epidemic by Joint United Nations Programme HIV/AIDS (UNAIDS) Sustainable Development Goals (SDGs). Precise HIV information critical effective geographic epidemiological targeting prevention treatment interventions. Age- sex-specific prevalence estimates are widely available...
We developed viral sensor and restriction factor-cytometry by time of flight (VISOR-CyTOF), which profiles 19 sensors factors (VISORs) simultaneously in single cells, applied it to 41 postmortem tissues from people with HIV. Mucosal myeloid cells are well equipped SAMHD1 capsid DNA while CD4
Diabetes is a metabolic disorder whose complications are among the leading cause of death. In this study, antidiabetic effect L-alanine was tested in alloxan-induced diabetic rats. Thirty-five male albino Wistar rats were divided into five groups viz; Group I and II: nondiabetic controls respectively; III IV: 150 300 mg/kg b.w. treated, V: glibenclamide (0.5 b.w.) treated. Weight blood glucose monitored during while liver kidney functions, lipid profile, antioxidant markers examined at end...
Sterile alpha motif and histidine-aspartic acid domain-containing protein 1 (SAMHD1) is a dNTP triphosphohydrolase involved in the regulation of intracellular pool, linked to viral restriction, cancer development autoimmune disorders. SAMHD1 function regulated by phosphorylation through mechanism controlled cyclin-dependent kinases tightly cell cycle progression. Recently, has been shown decrease efficacy nucleotide analogs used as chemotherapeutic drugs. Here, we demonstrate that can...
Any successful strategy to prevent and control HCV infection requires an understanding of the epidemic behaviour among different genotypes. Here, we performed first characterization history transmission dynamics subtypes in Portugal. Direct sequencing NS5B was on 230 direct-acting antiviral drugs (DAA)-treatment naïve patients Lisbon. Phylogenetic analysis used for subtyping cluster identification. Bayesian methods were reconstruct subtypes. Sequences analysed resistance-associated...
The urgency to curtail the devastating effects of ongoing COVID-19 Pandemic has led implementation several measures limit its spread, including movement restrictions and social distancing. As most developing countries rely solely on hospital visitations for their medical needs, this impediment assessing healthcare services compounded by low uptake telehealth could result in dire consequences. This is a cross-sectional study among Healthcare providers (HCP) consumers (HCC) Nigeria. We...
Purpose SAMHD1 is a deoxynucleotide triphosphate (dNTP) triphosphohydrolase which has been proposed as putative prognostic factor in haematological cancers and certain solid tumours, although with controversial data. Here, we evaluate function ovarian cancer, both vitro cancer patients. Methods expression was downregulated cell lines OVCAR3 SKOV3 by RNA interference. Gene protein changes immune signalling pathways were assessed. patients evaluated immunohistochemistry survival analysis...
Abstract Purpose The lack of validated surrogate biomarkers is still an unmet clinical need in the management early breast cancer cases that do not achieve complete pathological response after neoadjuvant chemotherapy (NACT). Here, we describe and validate use SAMHD1 expression as a prognostic biomarker residual disease vivo vitro . Methods was evaluated cohort patients with stage II-III treated NACT. Heterotypic 3D cultures including tumor immune cells were used to investigate molecular...
Allium sativum (Garlic) is indigenous to Asia, but largely cultivated in Nigeria.It has acquired a reputation folklore as formidable prophylactic and therapeutic agent.This study was undertaken evaluate the vitro antioxidant activity of three extracts [ aqueous (AE), ethyl acetate (EA) petroleum ether (PE)] garlic using two tests; ferric ion reducing power H 2 O scavenging capacity.The reductive each extract found be directly proportional its concentration (0.2 mg/ml -1 mg/ml) which followed...
A 4-year-old child born to an HIV-1 seronegative mother was diagnosed with HIV-1, the main risk factor being transmission from child's father who seroconverting at time of birth. In context a forensic investigation, we aimed identify source infection and date event. Samples were collected two points about 4 years after Partial segments three genes (gag, pol, env) sequenced maximum likelihood (ML) Bayesian methods used determine direction estimate transmission. Neutralizing antibodies...
The persistence of latent HIV reservoirs allows for viral rebound upon antiretroviral therapy interruption, hindering effective HIV-1 cure. Emerging evidence suggests that modulation innate immune stimulation could impact latency and contribute to the clearing reservoir. Here, reactivation capacity a subclass selective JAK2 inhibitors was characterized as potential novel therapeutic strategy Notably, reversed in non-clonal lymphoid myeloid vitro models also ex vivo CD4+ T cells from ART+...
We evaluated the effects of supplementation L-alanine and L-glutamine on blood glucose levels biochemical parameters in alloxan-induced diabetic rat. Forty-nine animals were distributed into seven equal groups. Except for non-diabetic control, diabetes was induced all groups by intravenous alloxan injection followed daily with amino acids 14 days. Weight monitored during supplementation, while such as liver renal functions, lipid profile, antioxidant markers post-intervention. A significant...
Abstract Background The economic crisis that began in 2008 has severely affected Southern (Greece, Italy, Portugal, Spain) Western European (SWE) countries of Europe (WE) and may have ongoing efforts to eliminate viral hepatitis. This study was conducted investigate the impact on burden HBV HCV disease. Methods Global Burden Diseases 2019 data were used analyse rates epidemiological metrics acute chronic infections SWE WE. Time series modelling performed quantify healthcare expenditure time...
Abstract In 2015, 257 million people lived with chronic hepatitis B infection. Portugal 1.4% of the population is chronically infected virus. Limited information available regarding genetic diversity, origin HBV in and its role dissemination worldwide. this study, we aimed to investigate epidemic history transmission dynamics genotypes that are endemic Portugal. pol gene was sequenced from 130 patients followed Lisbon. genotype A (HBV/A) most prevalent (n=54, 41.5%), by D [HBV/D; (n=44,...
In Portugal, the genetic diversity, origin of HBV and Portuguese role in dissemination worldwide were never investigated. this work, we studied epidemic history transmission dynamics genotypes that are endemic Portugal. pol gene was sequenced from 130 patients followed Lisbon. genotype A most prevalent (n = 54, 41.5%), by D 44, 33.8%), E 32, 24.6%). Spatio-temporal evolutionary reconstructed BEAST using a Bayesian Markov Chain Monte Carlo method, with GTR nucleotide substitution model, an...
Abstract Hepatitis C virus (HCV) infection remains a global health problem. Previously, the prevalence of NS5A resistance-associated substitutions (RASs) to elbasvir, new direct-acting antiviral (DAA) against viral protein was assessed by our group before its introduction into clinical use in Spain. However, origin, epidemic history, transmission dynamics, diversity and baseline RASs agents HCV-GT1a Spain remain unknown. A nationwide cross-sectional survey individuals chronically-infected...
Abstract Introduction Cyclin-dependent kinase (CDK) 4/6 inhibitors represent a new paradigm in the treatment of hormone receptor (HR) positive and HER-2 negative metastatic breast cancer (MBC). However, despite their evident clinical benefit, cellular molecular mechanisms underlying efficacy drug failure are still largely unknown. CDK4/6 block cyclin D1-CDK4/6 interaction, leading to inhibition cell cycle progression through G1/S phase inhibiting proliferation. SAMHD1 is dNTPase that...
Nucleos(t)ide analogues are commonly used in the treatment of infectious disease and cancer. SAMHD1 is a deoxyribonucleotide (dNTP) triphosphohydrolase which involved regulation intracellular dNTP pool, whose function has been linked to viral restriction, cancer development, autoimmune disorders. Here, we evaluate on antiviral antiproliferative efficacy wide range nucleos(t)ide currently treat infections The anti-HIV-1 cytotoxic activity compounds was assessed primary established cell lines...
HIV latent infection may be associated with disrupted viral RNA sensing, interferon (IFN) signaling, and/or IFN stimulating genes (ISG) activation. Here, we evaluated the use of compounds selectively targeting at inhibitor nuclear factor-κB (IκB) kinase (IKK) complex subunits and related kinases (TBK1) as a novel pathway to reverse HIV-1 latency in latently infected non-clonal lymphoid myeloid cell vitro models. IKK inhibitors (IKKis) triggered up 1.8-fold increase reactivation both, The...