A5043999710- Hepatitis C virus research
- Biosensors and Analytical Detection
- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Hepatitis B Virus Studies
- Air Quality and Health Impacts
- Tryptophan and brain disorders
- Cytomegalovirus and herpesvirus research
- Long-Term Effects of COVID-19
- Respiratory viral infections research
- Climate Change and Health Impacts
- SARS-CoV-2 detection and testing
- Advanced biosensing and bioanalysis techniques
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Spectroscopy Techniques in Biomedical and Chemical Research
- COVID-19 Clinical Research Studies
- HIV-related health complications and treatments
- Environmental Justice and Health Disparities
- Microbial Natural Products and Biosynthesis
- RNA and protein synthesis mechanisms
- Biosimilars and Bioanalytical Methods
- Telomeres, Telomerase, and Senescence
- Genomics and Phylogenetic Studies
- SARS-CoV-2 and COVID-19 Research
Instituto de Salud Carlos III
2019-2025
Centro de Investigación Biomédica en Red
2025
Unidades Centrales Científico-Técnicas
2024
Universidad de Oviedo
2023
Centro Nacional de Microbiologia
2020-2022
Genome mining using standard bioinformatics tools has allowed for the uncovering of hidden biosynthesis gene clusters specialized metabolites in Streptomyces genomes. In this work, we have used an alternative approach consisting seeking "Streptomyces Antibiotic Regulatory Proteins" (SARP) encoding genes and analyzing their surrounding DNA region to unearth cryptic that cannot be identified tools. This strategy unveiling new ahb cluster argillaceus, which had not been retrieved before...
Airborne animal viral pathogens can rapidly spread and become a global threat, resulting in substantial socioeconomic health consequences. To prevent control potential epidemic outbreaks, accurate, fast, affordable point-of-care (POC) tests are essential. As proof-of-concept, we have developed molecular system based on the loop-mediated isothermal amplification (LAMP) technique for avian metapneumovirus (aMPV) detection, an airborne communicable agent mainly infecting turkeys chickens. For...
Around 10% of people with HIV (PWH) exhibit a low-level viremia (LLV) under antiretroviral therapy (ART). However, its origin and clinical significance are largely unknown, particularly at viremias between 50 200 copies/mL modern ART based on integrase strand transfer inhibitors (INSTIs). Our aim was to characterize their poor immune response against in comparison individuals suppressed (SV) non-HIV controls (NHC).
Coinfection with hepatitis C virus (HCV) influences HIV reservoir size. However, it is unknown whether this coinfection also induces a higher provirus transcription. Viral transcription promoted by synergy between cellular factors such as NF-κB and the viral regulator Tat. The impact of HCV on was analyzed in resting (r)CD4 T+ cells (CD3+CD4+CD25-CD69-HLADR-) rCD4 T cells-depleted PBMCs (rCD4 T- PBMCs) from multicenter cross-sectional study 115 cART-treated patients: 42 HIV+/HCV+ coinfected...
Hepatitis C virus (HCV) infection remains a global health problem, detected only in the early stages by molecular tests. Molecular tests detect HCV RNA, which is very prone to degradation ribonucleases, reason why blood samples must be transported and stored at - 20 °C, or even 70 °C for long-term storage. Flinders Technology Associates (FTA) cards are useful sampling collecting device dry spot (DBS) storage, especially low middle-income countries (LMIC). In this study, we analyzed viral RNA...
Background: Although human immunodeficiency virus type 1 (HIV-1) reservoir size is very stable under antiretroviral therapy (ART), individuals exposed to the Hepatitis C (HCV) (chronically coinfected and spontaneous clarifiers) show an increase in HIV spliced viral RNA, which could indicate that protein regulator Tat being more actively synthesized and, thus, lead a higher yield of new HIV. However, it still unknown whether effect HCV elimination with direct-acting antivirals (DAAs) modify...
ABSTRACT Background Both SARS-CoV-2 and HIV infection exhibit alterations in the senescence profile immune checkpoint (IC) molecules. However, midterm impact of on these profiles people with (PWH) remains unclear. This study aimed to evaluate differences plasma biomarker levels related ICs, senescence-associated secretory phenotype (SASP), pro- anti-inflammatory cytokines PWH following recovery from infection. Methods We conducted a cross-sectional 95 receiving antiretroviral therapy,...
Abstract Hepatitis C virus (HCV) infection remains a global health problem. Previously, the prevalence of NS5A resistance-associated substitutions (RASs) to elbasvir, new direct-acting antiviral (DAA) against viral protein was assessed by our group before its introduction into clinical use in Spain. However, origin, epidemic history, transmission dynamics, diversity and baseline RASs agents HCV-GT1a Spain remain unknown. A nationwide cross-sectional survey individuals chronically-infected...
Airbone viral pathogens can rapidly spread and become a global menace, including both human animal viruses which trigger important socioeconomical health effects. Therefore, to prevent contain potential epidemic outbreaks, accurate, fast, affordable diagnostic point-of-care tests (POCT) are required. As proof of concept, we have developed molecular detection system based in Loop-mediated isothermal AMPlification technique (LAMP) for two different RNA airborne viruses: the well-known...
Early diagnosis of active viral infections is crucial to prevent silent transmission among the population, as we have seen during present COVID-19 pandemic.Single stranded RNA viruses, including SARS-CoV-2, are currently detected using molecular methods, such gold standard qRT-PCR.These methods require expensive equipment and specialized training, which limits its use point-of-care (POC) diagnostic systems.We developed a novel, fast simple method, based in combination Isothermal Loop...
The mid-term impact of SARS-CoV-2 infection on senescence profile and immune checkpoints biomarkers in people living with HIV (PLWH) was assessed. Cross-sectional study 95 PLWH ART stratified by infection: a) 48 previously infected (PCR+) (HIV/SARS); b) 47 controls without previous (HIV). Plasma (n=44) related to cell checkpoint molecules, associated the Senescence-Associated Secretory Phenotype (SASP), pro anti-inflammatory cytokines were assessed Procartaplex Multiplex Immunoassays...
Abstract Previously, we identified a stabilization of the HIV reservoir size in + monoinfected and HIV+/HCV coinfected patients who eliminated HCV with DAAs as well decrease HIV+/HCV- spontaneously clearers. However, variations distribution memory CD4 T cell subpopulations after clearance remains unclear. To analyze expression pattern maturation (CD45RA CD27) senescence (CD28 CD57) markers by flow cytometry those patients. Longitudinal study (52 weeks follow-up) 49 HIV+/HCV+, 38 59 subjects,...