A5036519705- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Nuclear Structure and Function
- Neurological disorders and treatments
- Circadian rhythm and melatonin
- Neuroscience and Neural Engineering
- Microfluidic and Bio-sensing Technologies
- Exercise and Physiological Responses
- Receptor Mechanisms and Signaling
- Cancer Research and Treatments
- Acute Myeloid Leukemia Research
- Stress Responses and Cortisol
- Traditional Chinese Medicine Analysis
- Microtubule and mitosis dynamics
- Caveolin-1 and cellular processes
- Hepatitis C virus research
- Immunotherapy and Immune Responses
- Cancer Cells and Metastasis
- Acute Lymphoblastic Leukemia research
- Hematopoietic Stem Cell Transplantation
- T-cell and B-cell Immunology
- HIV/AIDS drug development and treatment
- Plant-based Medicinal Research
- Polysaccharides Composition and Applications
University of California, Los Angeles
2018-2023
Laboratoire d’immunologie intégrative du cancer
2018
Los Angeles City College
2018
Hologic (United States)
2016
Institute of Biomedical Sciences, Academia Sinica
1993
Abstract The role of transcription factors and biomolecules in cell type conversion has been widely studied. Yet, it remains unclear whether how intracellular mechanotransduction through focal adhesions (FAs) the cytoskeleton regulates epigenetic state reprogramming. Here, is shown that cytoskeletal structures mechanical properties cells are modulated during early phase induced neuronal (iN) reprogramming, with an increase actin assembly by Ascl1 transgene. reduction tension or adhesion at...
Critical functions of immune cells require them to rapidly change their shape and generate forces in response cues from surrounding environment.However, little is known about how soluble factors that may be present the microenvironment modulate key aspects cellular mechanobiology-such as cell deformability force generation-to impact such phagocytosis migration.Here we show signaling by stress hormones through b-adrenoceptors (b-AR) reduces macrophages; this dependent on changes organization...
Cell deformability is a label-free biomarker of cell state in physiological and disease contexts ranging from stem differentiation to cancer progression. Harnessing as phenotype for screening applications requires method that can simultaneously measure the hundreds samples interface with existing high throughput facilities. Here we present scalable filtration device, which relies on pressure-driven deformation cells through series pillars are separated by micron-scale gaps timescale seconds:...
DYT1 dystonia is a neurological movement disorder that caused by loss-of-function mutation in the DYT1/TOR1A gene, which encodes torsinA, luminal ATPase-associated (AAA+) protein. TorsinA required for assembly of functional linker nucleoskeleton and cytoskeleton (LINC) complexes, consequently mechanical integration nucleus cytoskeleton. Despite potential implications altered mechanobiology pathogenesis, role torsinA regulating cellular phenotype, or mechanotype, remains unknown. Here, we...
Cancer cell mechanotype changes are newly recognized cancer phenotypic events, whereas metastatic cells show decreased stiffness and increased deformability relative to normal cells. To further examine how in early stages of transformation progression, an vitro multi-step human urothelial carcinogenic model was used measure cellular Young’s modulus, deformability, transit time using single-cell atomic force microscopy, microfluidic-based cytometry, quantitative respectively. Measurable...
The recurrence of cancer following chemotherapy treatment is a major cause death across solid and hematologic cancers. In B-cell acute lymphoblastic leukemia (B-ALL), relapse after initial leads to poor patient outcomes. Here we test the hypothesis that chemotherapy-treated versus control B-ALL cells can be characterized based on cellular physical phenotypes. To quantify phenotypes cells, use derived from patients are treated for 7 days with standard multidrug regimen vincristine,...
Abstract Cell reprogramming has wide applications in tissue regeneration, disease modeling and personalized medicine, but low efficiency remains a challenge. In addition to biochemical cues, biophysical factors can modulate the epigenetic state variety of cell functions. However, how help overcome barrier for are not well understood. Here we utilized microfluidic channels induce transient deformation nucleus, which caused disassembly nuclear lamina downregulation DNA methylation histone...
Abstract DYT1 dystonia is a neurological movement disorder that caused by loss-of-function mutation in the / TOR1A gene, which encodes torsinA, luminal ATPase-associated (AAA+) protein. TorsinA required for assembly of functional linker nucleoskeleton and cytoskeleton (LINC) complexes, consequently mechanical integration nucleus cytoskeleton. Despite potential implications altered mechanobiology pathogenesis, role torsinA regulating cellular phenotype, or mechanotype, remains unknown. Here,...
Background: Endothelial-to-mesenchymal transition (EndMT), the process whereby endothelial cells (ECs) into multipotent stem cell-like cells, has been implicated in a variety of cardiovascular pathologies, including calcification. EndMT is dynamic mechanical that involves detachment transitioning ECs from adjacent and active migration these endothelium interstitium. While gene protein expression profiles undergoing have well characterized, less known about properties, or “mechanotype,” as...