A5019103268- Estrogen and related hormone effects
- Hormonal Regulation and Hypertension
- Reproductive Biology and Fertility
- Aldose Reductase and Taurine
- Hypothalamic control of reproductive hormones
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Renal and related cancers
- DNA Repair Mechanisms
- Pharmacogenetics and Drug Metabolism
- Steroid Chemistry and Biochemistry
- Sperm and Testicular Function
- Urological Disorders and Treatments
- Reproductive System and Pregnancy
- Ovarian function and disorders
- Genomics and Chromatin Dynamics
- BRCA gene mutations in cancer
- Cell Adhesion Molecules Research
- Sexual Differentiation and Disorders
- Retinoids in leukemia and cellular processes
- Cancer-related Molecular Pathways
- CRISPR and Genetic Engineering
- Tissue Engineering and Regenerative Medicine
- Ovarian cancer diagnosis and treatment
- Epigenetics and DNA Methylation
- Hormonal and reproductive studies
University of Oulu
2006-2024
Cancer Genetics (United States)
2022
Imperial College London
2005-2021
NordLab
2018-2019
Hammersmith Hospital
2005-2018
World Health Organization
2000
World Health Organization - Pakistan
1998
Oulu University Hospital
1994
University of Cambridge
1994
CDNA clones for 17β‐hydroxysteroid dehydrogenase (17‐HSD; EC 1.1.1.62) were isolated from a placental λgt11 expression library using polyclonal antibodies against 17‐HSD. The largest cDNA contained 1325 nucleotides, consisting of short 5′‐noncoding segment, coding segment 987 nucleotides terminated by TAA codon, and 329 nucleotide long 3′‐noncoding segment. open reading frame encoded polypeptide 327 amino acid residues with predicted M r 34853. sequence 23 N‐terminal acids determined...
Wnt-4 is a signaling factor with multiple roles in organogenesis, deficiency that leads to abnormal development of the kidney, pituitary gland, female reproductive system, and mammary gland. expressed cortical region developing adrenal gland from embryonic d 11.5 onward, especially outermost part. Expression Cyp11B2 preadipocyte 1 lowered glands mutant animals, resulting significantly reduced aldosterone production newborn mutants, suggesting may be needed for proper formation zona...
ABSTRACT Epithelial-mesenchymal tissue interactions regulate the formation of signaling centers that play a role in coordination organogenesis, but it is not clear how their activity leads to differences organogenesis. We report type XVIII collagen, which contains both frizzled and an endostatin domain, expressed throughout respective epithelial bud at initiation lung kidney It becomes localized tips during early stages branching, while its expression confined stalk region lost from nearly...
Abstract 17β-Hydroxysteroid dehydrogenases/17-ketosteroid reductases (17HSDs) modulate the biological activity of certain estrogens and androgens by catalyzing reductase or dehydrogenase reactions between 17-keto- 17β-hydroxysteroids. In present study, we demonstrate expression cloning a novel type 17HSD, chronologically named 17HSD 7, from HC11 cell line derived mouse mammary gland. The cloned cDNA, 1.7 kb in size, encodes protein 334 amino acids with calculated molecular mass 37,317 Da....
Rapid non-genomic actions of progesterone are implicated in many aspects female reproduction. Recently, three human homologues the fish membrane progestin receptor (mPR) have been identified. We combined bioinformatic analysis with expression profiling to define further role these mPRs reproductive tissues. Sequence confirmed that belong a larger, highly conserved family proteins, termed 'progestin and adiponectin receptors' (PAQRs). A comparison mPR transcripts two related PAQR members,...
Spermatogenesis is regulated by the 2 pituitary gonadotropins, luteinizing hormone (LH) and follicle-stimulating (FSH). This process considered impossible without absolute requirement of LH-stimulated testicular testosterone (T) production. The role FSH remains unclear because men mice with inactivating receptor (FSHR) mutations are fertile. We revisited in spermatogenesis using transgenic expressing a constitutively strongly active FSHR mutant LH receptor–null (LHR-null) background....
Wnt-4 signaling has been implicated in female development, because its absence leads to partial male sex reversal the mouse. Instead of Mullerian ducts, Wnt-4-deficient females have Wolffian suggesting a role for androgens maintaining this single-sex duct type females. We demonstrate here that testosterone is produced by ovary embryos and also detected embryonic plasma. Consistent with this, expression several genes encoding enzymes pathway leading synthesis mouse induced ovary, including...
Abstract Epithelial–mesenchymal tissue interactions play a central role in vertebrate organogenesis, but the molecular mediators and mechanisms of these morphogenetic are still not well characterized. We report here on expression pattern Wnt‐2b during mouse organogenesis tests its function epithelial– mesenchymal kidney development. is expressed numerous developing organs embryo, including kidney, lung, salivary gland, gut, pancreas, adrenal genital tubercle. Additional sites include...
The biological activity of certain estrogens and androgens is modulated by enzymes called 17β‐hydroxysteroid dehydrogenases (17β‐HSDs), which catalyze the interconversion between less active 17‐oxosteroid more forms. In present report, we describe cloning mouse 17β‐HSD type‐1 cDNA from an ovarian library generated 4,4′‐(1,2‐diethyl‐1,2‐ethenediyl)bisphenol‐(diethylstilbestrol)‐treated mice, characterization corresponding enzyme. open reading frame encodes a peptide 344 amino acid residues...
Several amino acid residues (Cys54, Tyr155, His210, His213 and His221) at a putative catalytic site of human 17 beta-hydroxysteroid dehydrogenase type 1 were mutated to Ala. Replacement His221 by Ala remarkably reduced the activity, which resulted from change both Km Vmax. values enzyme. Compared with wild-type enzyme, efficiency His221-->Ala mutant was 20-fold for oxidative reaction 11-fold reductive reaction. With similar mutations His210 or His213, no notable effects on properties...
The association of primary amenorrhea, gonadotropin levels at menopausal range, and normal antral follicle endowment is a rare clinical condition that suggests unresponsiveness follicles to FSH. This affection frequently misdiagnosed ovarian failure patients are referred egg donation treat their infertility. Because these notably have an age-compatible number follicles, we hypothesized in vitro maturation (IVM) oocytes might constitute useful approach for treating infertility.We report the...
Strong gain-of-function mutations have not been identified in humans the FSH receptor (FSHR), whereas such are common among many other G protein-coupled receptors. In order to predict consequences of on humans, we first constitutively activated mutants mouse (m) Fshr and then expressed them under human anti-Müllerian hormone promoter transgenic mice or created knock-in mutation into genome. We show here that Asp580 mFSHR significantly increase basal activity. D580H D580Y bind FSH, but...
The tumor extracellular matrix (ECM) critically regulates cancer progression and treatment response. Expression of the basement membrane component collagen XVIII (ColXVIII) is induced in solid tumors, but its involvement tumorigenesis has remained elusive. We show here that ColXVIII was markedly upregulated human breast (BC) closely associated with a poor prognosis high-grade BCs. discovered role for as modulator epidermal growth factor receptor tyrosine kinase (ErbB) signaling it forms...
The 5′-flanking region from −78 to +9 in the HSD17B1 gene serves as a promoter, and an silencer element is located position −113 −78. In present studies, we have characterized three regulatory elements proximal regions of gene, using electrophoretic mobility shift assays reporter analysis. First, nuclear factors recognized by antibodies against Sp1 Sp3 were found bind motif −52 −43. Mutation Sp1-binding site decreased promoter activity 30% JEG-3 cells 60% JAR cells, suggesting that binding...
The Wnt family member Wnt4 is critical for the development of several organs, including kidney, gonad, and adrenal, mammary, pituitary glands. To study its potential postnatal functions, we generated a floxed allele. A single loxP site was targeted to second intron, while loxP-Neo-loxP cassette placed 3' from Exon 5. Neo subsequently removed by crossing with MeuCre40 transgenic mice. gene specifically inactivated CAGCre another allele, Wnt4(EGFPCre), in which Cre driven endogenous promoter....