A5083885611- Antibiotic Resistance in Bacteria
- Urological Disorders and Treatments
- Renal and related cancers
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacteriophages and microbial interactions
- Bacterial biofilms and quorum sensing
- Bacterial Identification and Susceptibility Testing
- Genomics and Phylogenetic Studies
- Enterobacteriaceae and Cronobacter Research
- Salmonella and Campylobacter epidemiology
- Tissue Engineering and Regenerative Medicine
- Infections and bacterial resistance
- Molecular Biology Techniques and Applications
- Respiratory viral infections research
- Reproductive Biology and Fertility
- Cell Adhesion Molecules Research
- Gut microbiota and health
- RNA modifications and cancer
- Neonatal Respiratory Health Research
- Mycobacterium research and diagnosis
- Virus-based gene therapy research
- Birth, Development, and Health
- Cytokine Signaling Pathways and Interactions
- Peptidase Inhibition and Analysis
- PI3K/AKT/mTOR signaling in cancer
Affiliated Hospital of Nantong University
2025
Nantong University
2025
Guangzhou University of Chinese Medicine
2024
Fujian Medical University
2018-2024
Huadong Hospital
2023-2024
Gene Tech (China)
2024
Academy of Military Medical Sciences
2019-2023
Chinese Center For Disease Control and Prevention
2020-2023
Chinese People's Liberation Army
2023
Beijing Center for Disease Prevention and Control
2019-2022
Retinoic acid (RA) is an essential extrinsic inducer of meiotic initiation in mammalian germ cells. However, RA acts too widely development to account, by itself, for the cell-type and temporal specificity initiation. We considered parallels yeast, which intrinsic factors combine restrict demonstrate that, mouse embryos, together regulate The RNA-binding protein DAZL, expressed postmigratory cells, a key factor, enabling those cells initiate meiosis response RA. Within brief developmental...
Mammalian oocytes and spermatozoa derive from fetal cells shared by the sexes. These primordial germ (PGCs) migrate to developing somatic gonad, giving rise or spermatozoa. opposing sexual fates are determined not PGCs’ own sex chromosome constitution (XX XY), but identity of gonad that they enter. We asked whether PGCs undergo a developmental transition enables them respond feminizing masculinizing cues ovary testis. conducted in vivo genetic studies DAZL, an RNA-binding protein expressed...
ABSTRACT Epithelial-mesenchymal tissue interactions regulate the formation of signaling centers that play a role in coordination organogenesis, but it is not clear how their activity leads to differences organogenesis. We report type XVIII collagen, which contains both frizzled and an endostatin domain, expressed throughout respective epithelial bud at initiation lung kidney It becomes localized tips during early stages branching, while its expression confined stalk region lost from nearly...
The kidney is a classic model for studying mechanisms of inductive tissue interactions associated with the epithelial branching common to many embryonic organs, but molecular are still poorly known. Sprouty proteins antagonize tyrosine kinases in Egf and Fgf receptors candidate components signalling as well. We have addressed function sprouty vivo by targeted expression human 2 (SPRY2) ureteric bud, which normally expresses signals mouse (Spry2). Ectopic SPRY2 led postnatal death resulting...
The embryonic kidney is a classic developmental model system for studying inductive tissue interactions that govern organogenesis. We report here Wnt-6 expressed in the ureter bud, and cell lines expressing induce nephrogenesis vitro. cells tubules with similar kinetics to spinal cord (SPC) lead induced expression of Pax2, Pax8, Sfrp2, E-cadherin genes, early markers tubulogenesis. Moreover, signaling rescues tubulogenesis mesenchyme separated from Wnt-4 mutant embryos leads activation...
Background: Berberine has extremely low oral bioavailability, but shows a potent lipid-lowering effect, indicating its potential role in regulating intestinal microbiota, which not been investigated. Methods: In the present study, five experimental diets, control diet (Con), high-lipid (HL), and high-lipid·diets·supplemented with an antibiotic cocktail (HLA), berberine (HLB), or both (HLAB) were fed to zebrafish (Danio rerio) for 30 days. Results: The HLB group showed significantly greater...
Abstract Epithelial–mesenchymal tissue interactions play a central role in vertebrate organogenesis, but the molecular mediators and mechanisms of these morphogenetic are still not well characterized. We report here on expression pattern Wnt‐2b during mouse organogenesis tests its function epithelial– mesenchymal kidney development. is expressed numerous developing organs embryo, including kidney, lung, salivary gland, gut, pancreas, adrenal genital tubercle. Additional sites include...
Calponin 2 (h2 calponin, CNN2) is an actin-binding protein implicated in cytoskeletal organization. We have found that the expression of calponin relatively restricted to vasculature from 16 30 h post-fertilization during zebrafish (Danio rerio) development. Forty-eight hours after injecting antisense morpholino oligos against into embryos at 1-4-cell stage, demonstrated various cardiovascular defects, including sluggish axial and head circulation, absence circulation intersegmental vessels...
We report the draft genome sequence of Achromobacter arsenitoxydans SY8, first reported arsenite-oxidizing bacterium belonging to genus and containing a genomic arsenic island, an intact type III secretion system, multiple metal(loid) transporters. The may be helpful explore mechanisms intertwining resistance pathogenicity.
Influenza A virus (IAV)-induced dysbiosis may predispose to severe bacterial superinfections. Most studies have focused on the microbiota of single mucosal surfaces; consequently, relationships between at different anatomic sites in IAV-infected mice not been fully studied.We characterized respiratory and gut using full-length 16S rRNA gene sequencing by Nanopore sequencers compared nasopharyngeal, oropharyngeal, lung microbiomes healthy mice.The were dominated Lactobacillus spp., while...
Genomic surveillance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) plays an important role in COVID-19 pandemic control and elimination efforts, especially by elucidating its global transmission network illustrating viral evolution. The deployment multiplex PCR assays that target SARS-CoV-2 followed either massively parallel or nanopore sequencing is a widely-used strategy to obtain genome sequences from primary samples. However, PCR-based carries inherent bias depth among...
Nanopore sequencing has been widely used in clinical metagenomic for pathogen detection with high portability and real-time sequencing. Oxford Technologies recently launched an adaptive function, which can enrich on-target reads through alignment eject uninteresting by reversing the voltage across nanopore. Here we evaluated utility of detection.Nanopore standard was performed on a same flow cell bronchoalveolar lavage fluid sample from patient Chlamydia psittacosis infection, compared...
Rapid and accurate etiologic diagnosis accelerates targeted antimicrobial therapy. Metagenomic analysis has played a critical role in pathogen identification. In this study, we leveraged the advantages of both MinION BGISEQ-500 platforms to make bacteriologic from culture-negative lung tissue sample an immunocompromised patient with severe pneumonia. Real-time nanopore sequencing rapidly identified Klebsiella pneumoniae by 823bp specific sequence within 1 minute. Genomic further...
Nanopore sequencing has been widely used for the real-time detection and surveillance of pathogens with portable MinION. adaptive can enrich on-target sequences without additional pretreatment. In this study, performance was evaluated viral genome enrichment clinical respiratory samples. Ligation-based nanopore (LNAS) rapid PCR-based (RPNAS) workflows were performed to assess effects on nasopharyngeal swab samples from human adenovirus (HAdV) outbreaks. RPNAS further applied severe acute...
The emergence of carbapenem-resistant Enterobacteriaceae poses a significant threat to public health worldwide. Here, we reported multidrug-resistant Escherichia coli strain with two different blaNDM-5-carrying plasmids from China. Illumina short-read and MinION long-read whole genome sequencing were performed. Genomic analysis found that one blaNDM-5 gene together mphA was located on 55-kb IncX3 plasmid, while the other novel 68-kb IncFII plasmid. Susceptibility testing quantitative reverse...
Abstract Background Chlamydia psittaci is an avian pathogen that can cause lethal human infections. Diagnosis of C. pneumonia often delayed due to nonspecific clinical presentations and limited laboratory diagnostic techniques. Results The MinION platform established the diagnosis in shortest time, while BGISEQ-500 generated additional in-depth sequence data included rapid characterization antibiotic susceptibility. Cytopathy appeared only cell cultures BALF. BALF yielded a higher bacterial...