A5100432689- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Salmonella and Campylobacter epidemiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Enterobacteriaceae and Cronobacter Research
- Bacteriophages and microbial interactions
- Bacterial biofilms and quorum sensing
- Viral gastroenteritis research and epidemiology
- Genomics and Phylogenetic Studies
- Infections and bacterial resistance
- Hydrogels: synthesis, properties, applications
- Listeria monocytogenes in Food Safety
- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Bacterial Identification and Susceptibility Testing
- SARS-CoV-2 and COVID-19 Research
- Curcumin's Biomedical Applications
- Mycobacterium research and diagnosis
- Gut microbiota and health
- Digestive system and related health
- Pesticide Residue Analysis and Safety
- Melamine detection and toxicity
- Probiotics and Fermented Foods
- Proteins in Food Systems
- Molecular Biology Techniques and Applications
Chinese Center For Disease Control and Prevention
2020-2024
Chinese People's Liberation Army
2020-2023
Tomas Bata University in Zlín
2023
Beijing Center for Disease Prevention and Control
2018-2022
Center for Disease Control
2020
Henan University of Technology
2020
Beijing Institute of Technology
2016-2019
Academy of Military Medical Sciences
2013-2018
Thomas Jefferson University
2007-2017
National Institute of Biological Sciences, Beijing
2017
Multidrug resistant microbes present in the environment are a potential public health risk. In this study, we investigate presence of New Delhi metallo-β-lactamase 1 (NDM-1) producing bacteria 99 water samples Beijing City, including river water, treated drinking raw from pools and sewage 4 comprehensive hospitals. For blaNDM-1 positive isolate, antimicrobial susceptibility testing was further analyzed, Pulsed Field Gel Electrophoresis (PFGE) performed to determine genetic relationship among...
Curcuma is a world-renowned herb known for its immense health benefits. In this study, physicochemical analyses were performed on the curcumin standard sample and multiple emulsions. The emulsions analysed thermal structural stability 21 days. Confocal laser microscopy (CLSM) was in order to observe emulsion encapsulation. Modulated differential scanning calorimetry (MDSC) HPLC methods revealed variety of curcuminoids (curcumin, demethoxycurcumin, bisdemethoxycurcumin, cyclocurcumin)...
Shigella flexneri serotype 2 variant (II:3,4,7,8) was isolated in 2008 and first reported China 2013. In the present study, epidemiological surveillance from 2003 to 2013 suggested that this appeared Guangxi 2003; it then emerged Shanghai Xinjiang 2004 Henan 2008. Of 1813 S. isolates, 58 strains were identified. Serotype has as a prominent recent years, with 2a (32.6%), X (25.2%), 1a (9.4%), (6.3%), 2b (5.4%), 1b (3.6%). According phenotypic genotypic analysis, originated 2b. A higher...
The emergence of carbapenemase-producing Citrobacter freundii poses a significant threat to public health worldwide. Here, we reported C. strain CWH001 which was resistant all tested antimicrobials except tetracycline. Whole genome sequencing and analysis were performed. strain, belonged new sequence type ST139, showed close relationship with other foreign strains through phylogenetic analysis. A novel variant the intrinsic blaCMY gene located on chromosome identified designated as...
Listeria monocytogenes is a significant foodborne pathogen causing severe systemic infections in humans with high mortality rates. The objectives of this work were to establish phylogenetic framework L. from China and investigate sequence diversity among different serotypes. We selected 17 strains recovered patients foods representing serotypes 1/2a, 1/2b, 1/2c. Draft genome sequences determined using Illumina MiSeq technique associated protocols. Open reading frames assigned prokaryotic...
Here, we report for the first time a waterborne outbreak of Shigella sonnei in China 2015. Eleven multidrug-resistant (MDR) S. isolates were recovered, showing high resistance to azithromycin and third-generation cephalosporins particular, due an mph(A)- blaCTX-M-14-harboring IncB/O/K/Z group transmissible plasmid 104,285 kb size. Our study highlights potential prevalence MDR its further dissemination worldwide with development globalization.
Abstract To conduct the first comprehensive analysis of Shigella flexneri serotype 4s, a novel found in 2010, we identified 24 4s isolates from 1973 shigellosis cases China (2002–2014). The were characterized by single nucleotide polymorphism (SNP) phylogenetic analysis, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) to determine their genetic relatedness analysed further for antimicrobial susceptibilities resistance determinants. PFGE SNP analyses suggest that...
Since the gene encoding mobilised colistin resistance (mcr-1) was first reported in China 2015, it has been various Enterobacteriaceae worldwide. Escherichia coli, one of main pathogens causing diarrhoea, is most prevalent, clinically identified species carrying mcr-1. This study aimed to investigate epidemiologic and genomic characteristics mcr-1 coli from patients Shanghai.Faecal samples were collected hospitals Shanghai between 2012 2015. Polymerase chain reaction performed detect mcr-1,...
Rapid and accurate etiologic diagnosis accelerates targeted antimicrobial therapy. Metagenomic analysis has played a critical role in pathogen identification. In this study, we leveraged the advantages of both MinION BGISEQ-500 platforms to make bacteriologic from culture-negative lung tissue sample an immunocompromised patient with severe pneumonia. Real-time nanopore sequencing rapidly identified Klebsiella pneumoniae by 823bp specific sequence within 1 minute. Genomic further...
Shigella flexneri serotype 1b is among the most prominent serotypes in developing countries, followed by 2a. However, only limited data available on global phenotypic and genotypic characteristics of S. 1b. In present study, 40 isolates from different regions China were confirmed serotyping biochemical characterization. Antimicrobial susceptibility testing showed that 85% these multidrug-resistant strains antibiotic profiles varied between geographical locations. Strains Yunnan far more...
We have recovered one blaNDM-1-harboring bacterial strain, designated as XM1570, from a sputum sample obtained fatal case of pneumonia in China. Biochemical profiling, 16S rRNA sequencing and antimicrobial susceptibility testing were performed. Conjugation experiments conducted to determine transmissibility resistance. Pulsed-field gel electrophoresis whole genome performed identify strain-specific features. The isolate XM1570 was identified Acinetobacter calcoaceticus. Whole two plasmids,...
The prevalence and dissemination of diverse NDM-producing bacteria in China was investigated.We collected 1,162 isolates from 8 cities during December 2013∼May 2015 China. NDM-positive strains as well the NDM genotypes these sample were detected via Vitek 2 compact system (bioMérieux, France), 16S rRNA gene sequencing, PCR an S1- pulsed-field gel electrophoresis assay Southern blot hybridization. horizontal-transfer capability blaNDM assessed by filter mating using a standard E.coli J53...
The emergence of carbapenem-resistant Enterobacteriaceae poses a significant threat to public health worldwide. Here, we reported multidrug-resistant Escherichia coli strain with two different blaNDM-5-carrying plasmids from China. Illumina short-read and MinION long-read whole genome sequencing were performed. Genomic analysis found that one blaNDM-5 gene together mphA was located on 55-kb IncX3 plasmid, while the other novel 68-kb IncFII plasmid. Susceptibility testing quantitative reverse...
Shigella flexneri is an important cause of bacillary dysentery in developing countries. Small regulatory RNAs (sRNAs) play essential roles diverse cellular processes. We found a novel sRNA Ssr1 based on RT-PCR, northern blot, and 5´RACE S. flexneri. responds to acidic environmental changes, as shown by strong linear correlation between the pH value expression (R = 0.785, P < 0.05) using qRT-PCR method. Deletion results growth retardation at values ranging from 5.0 7.0 (P 0.05), survival rate...
Abstract We identified 3 atypical Shigella flexneri varieties in China, including 92 strains with multidrug resistance, distinct pulse types, and a novel sequence type. Atypical were prevalent mainly developed regions, 1 variant has become the dominant spp. serotype China. Improved surveillance will help guide prevention control of shigellosis.