A5061058009- Single-cell and spatial transcriptomics
- Neurogenesis and neuroplasticity mechanisms
- Childhood Cancer Survivors' Quality of Life
- Family Support in Illness
- CRISPR and Genetic Engineering
- Immune responses and vaccinations
- Cancer Cells and Metastasis
- Inflammatory Bowel Disease
- Digital Imaging in Medicine
- Neuroinflammation and Neurodegeneration Mechanisms
- CAR-T cell therapy research
- Cancer Genomics and Diagnostics
- 3D Printing in Biomedical Research
- Phagocytosis and Immune Regulation
- Evolution and Genetic Dynamics
- Pancreatic function and diabetes
- Immune cells in cancer
- Colorectal Cancer Screening and Detection
- Health Systems, Economic Evaluations, Quality of Life
ETH Zurich
2023-2024
Adult neural stem cells (NSCs) contribute to lifelong brain plasticity. In the adult mouse ventricular-subventricular zone, NSCs are heterogeneous and, depending on their location in niche, give rise different subtypes of olfactory bulb (OB) interneurons. Here, we show that multiple regionally distinct NSCs, including domains usually quiescent, recruited gestation days during pregnancy. Synchronized activation these NSC pools generates transient waves short-lived OB interneurons, especially...
It is estimated that only 0.02% of disseminated tumour cells are able to seed overt metastases
The tumour evolution model posits that malignant transformation is preceded by randomly distributed driver mutations in cancer genes, which cause clonal expansions phenotypically normal tissues. Although can remodel entire tissues
Abstract Sequencing-based spatial transcriptomics (ST) methods allow unbiased capturing of RNA molecules at barcoded spots, charting the distribution and localization cell types transcripts across a tissue. While coarse resolution these techniques is considered disadvantage, we argue that inherent proximity transcriptomes captured on spots can be leveraged to reconstruct cellular networks. To this end, developed ISCHIA (Identifying Spatial Co-occurrence in Healthy InflAmed tissues),...
Spatial transcriptomics techniques are able to chart the distribution and localization of cell types RNA molecules across a tissue. Here, we generated matched sequencing-based (Visium) hybridization-based (Molecular Cartography) spatial data human IBD samples. We then developed ISCHIA (Identifying Co-occurrence in Healthy InflAmed tissues), computational framework analyze co-occurrence transcript species tissue environment. revealed tightly associated cellular networks, ligand-receptor...
The tumor evolution model posits that malignant transformation is preceded by randomly distributed driver mutations in cancer genes, which cause clonal expansions phenotypically normal tissues. Although occur frequently human epithelia and can remodel almost entire tissues, the mechanisms behind why only a small number of clones transform into tumors remain enigmatic. Here, we develop an vivo single-cell CRISPR strategy to systematically investigate tissue-wide dynamics 150 most mutated...
Abstract It is estimated that only 0.02% of disseminated tumor cells are able to seed overt metastases 1 . While this indicates the presence environmental constraints metastatic seeding, landscape host factors controlling process remains largely unknown. Combining transposon technology 2 and fluorescent niche labeling 3 , we developed an in vivo CRISPR activation screen systematically investigate influence hepatocytes on seeding liver. Our approach enabled identification Plexin B2 as a...