A5084718927- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Porphyrin and Phthalocyanine Chemistry
- RNA Interference and Gene Delivery
- Click Chemistry and Applications
- Chemical Synthesis and Analysis
- RNA and protein synthesis mechanisms
- Synthesis and biological activity
- Synthesis and Characterization of Heterocyclic Compounds
- Metal complexes synthesis and properties
- Synthesis and Biological Evaluation
- RNA modifications and cancer
- Crystallization and Solubility Studies
- Luminescence and Fluorescent Materials
- X-ray Diffraction in Crystallography
- Biosensors and Analytical Detection
- Analytical Chemistry and Chromatography
- Molecular Junctions and Nanostructures
- Mass Spectrometry Techniques and Applications
- Molecular Sensors and Ion Detection
- Photodynamic Therapy Research Studies
- Metal-Catalyzed Oxygenation Mechanisms
- Biopolymer Synthesis and Applications
- Analytical chemistry methods development
- Biochemical and Molecular Research
Institute of Molecular Biology and Genetics
2013-2024
National Academy of Sciences of Ukraine
2011-2024
Centre National de la Recherche Scientifique
1998-2002
Université Toulouse III - Paul Sabatier
2002
Laboratoire de Chimie de Coordination
1998-2001
Institute of Bioorganic Chemistry and Petrochemistry V.P. Kukhar
1992-2000
Institute of Bioorganic Chemistry
1986-1990
Ab initio quantum-chemical study of specific point contacts replisome proteins with DNA modeled by acetic acid canonical and mutagenic tautomers bases methylated at the glycosidic nitrogen atoms was performed in vacuo continuum a low dielectric constant (ϵ ∼ 4) corresponding to hydrophobic interface protein–nucleic interaction. All tautomerized complexes were found be dynamically unstable, because electronic energies their back-reaction barriers do not exceed zero-point vibrational...
The combination of the generic mobile technology and inherent stability, versatility cost-effectiveness synthetic receptors allows producing optical sensors for potentially any analyte interest, and, therefore, to qualify as a platform fast routine analysis large number contaminated samples. To support this statement, we present here novel miniature sensor based on molecularly imprinted polymer (MIP) membranes smartphone, which could be used point-of-care detection an important food...
A copper-promoted reductive homocoupling reaction, for the first time used a metal complex, allowed obtaining new kind of complexes with encapsulated ions, C–C conjugated bis-clathrochelates. These compounds demonstrate extremely high transcription inhibition activity in T7 RNA polymerase system values IC50 reaching as low submicromolar range, which places them among most potent metal-based inhibitors.
A novel enhanced fluorescent sensor system for zearalenone (ZON) determination in flour samples is presented. The ZON-selective molecularly imprinted polymer (MIP) films were developed with a computational modelling method and synthesised cyclododecyl-2,4-dihydroxybenzoate as "dummy" template ethylene glycol methacrylate phosphate functional monomer acted the selective recognition elements ZON fluorescence detection. Spherical silver nanoparticles (AgNPs) embedded MIP films' structure to...
The Mn-TMPyP/KHSO5 system was used to oxidize guanine contained within a dinucleoside monophosphate d(GpT). To identify the oxidation product having mass with 4 amu above of itself, this relatively unstable compound reduced more stable one. ESI/MS and NMR data allowed us propose dehydro-guanidinohydantoin structure for (G+4) product.
An effective procedure for the synthesis of oligonucleotides by phosphotriester method has been developed. The is based on use phosphate protecting groups enabling O-nucleophilic intramolecular catalysis in reaction internucleotide bond formation under action arylsulfonyl chlorides and their derivatives. Using this new procedure, time needed to perform one elongation step polymer support 7-8 min. effectiveness methodology demonstrated many oligodeoxyribonucleotides different length with high yields.
Coumarin-based fluorescent agents play an important role in the manifold fundamental scientific and technological areas need to be carefully studied. In this research, linear photophysics, photochemistry, fast vibronic relaxations, two-photon absorption (2PA) of coumarin derivatives, methyl 4-[2-(7-methoxy-2-oxo-chromen-3-yl)thiazol-4-yl]butanoate (1) 4-[4-[2-(7-methoxy-2-oxo-chromen-3-yl)thiazol-4-yl]phenoxy]butanoate (2), were comprehensively analyzed using stationary time-resolved...
The 5'-aldehyde terminus is a DNA oxidative damage resulting from attack at C5' of 2-deoxyriboses by some potent natural or chemical cleavers. To offer fast and specific method for characterization this type damage, we used on-line electrospray ionization mass spectrometry (ESI-MS) detection during liquid chromatography analyses. intrinsic reactivity with nucleophiles (formation hydrate water, Tris adduct buffer) through β-elimination reaction resulted in complex LC profiles MS data. We...
Spectral properties, energy structure, and electronic processes in DNA, poly(dAdT)2, d(CCCGGGTTTAAA), d(ATC), d(AT), dGMP, dAMP, dCMP, dTMP were studied. The DNA lowest excited singlet triplet levels are connected with guanine adenine bases, correspondingly. fluorescence is close to a linear combination of the cytidine emissions connected, greater extent, transfer these bases. origin phosphorescence associated AT-sequences that traps excitations DNA. centers bases (that most photostable)...
In this review we have summarized the results of our recent research on telomerase inhibitors and G-quadruplex DNA ligands.A series potential enzyme were synthesized studied.These compounds based tricyclic heteroaromatic systems (thiazolobenzimidazoles phenazines, acridones), amino-substituted cyanines natural synthetic porphyrins their metalocomplexes.A number compounds, including especially porphyrin derivatives conjugates, found to efficiently inhibit at low micromolar concentrations in...
A new general method for an easy 2′-O-modification of nucleosides is reported. We describe the preparation modified 19-mer oligonucleotides carrying aminoalkyl linker at 2′-position cytidine residues and covalent attachment artificial chemical nuclease, a manganese(III) tris(N-methylpyridinium-4-yl)porphyrin moiety, onto functionalized linker. The positions manganese cationic porphyrin within vector sequence length tether were selected in order to have DNA-cleaver entity close (AT)3-sequence...
The interaction of a tricationic water-soluble meso-(N-methylpyridinium)-substituted porphyrin, TMPyP3+, derived from classic TMPyP4, with double-stranded poly(G) ⋅ poly(C) and four-stranded polyribonucleotides has been studied in aqueous buffered solutions, pH 6.9, low near-physiological ionic strengths wide range molar phosphate-to-dye ratios (P/D). To clarify the binding modes TMPyP3+ to biopolymers various spectroscopic techniques, including absorption polarized fluorescence...
Abstract The synthesis of 2′-deoxyribosylurea by thymidine oxidation with potassium permanganate followed alkaline hydrolysis intermediates is described. anomeric configuration the resulting products was studied NMR spectroscopy. ACKNOWLEDGMENTS I. D. indebted to Université Paul Sabatier (Toulouse) for a fellowship. Yannick Coppel (LCC-CNRS) gratefully acknowledged performing ROESY experiments.
Abstract The mechanism of the internucleotide condensation and side-reactions in H-phosphonate approach has been investigated. modification this method allowed to minimize during preactivation nucleotidic component developed.
We report a comprehensive quantum-chemical study on d(A)5·d(T)5 and d(G)5·d(C)5 DNA mini-helixes the Dickerson dodecamer d[CGCGAATTCGCG]. The research was performed to model evolution of spatial structure d(G)5 d(C)5 all way from vacuum water bulk. influence external factors such as presence counterions extent hydration included. Also, for comparison, limited calculations have been carried out dodecamer. has at density functional theory level using B97D3 ωB97XD exchange–correlation...