A5101648757- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Hematopoietic Stem Cell Transplantation
- Tissue Engineering and Regenerative Medicine
- Autophagy in Disease and Therapy
- Hydrogen's biological and therapeutic effects
- RNA Interference and Gene Delivery
- Effects of Radiation Exposure
- Periodontal Regeneration and Treatments
- Advanced biosensing and bioanalysis techniques
- Sphingolipid Metabolism and Signaling
- Spinal Cord Injury Research
- Carbon and Quantum Dots Applications
- MicroRNA in disease regulation
- Calcium signaling and nucleotide metabolism
- Neonatal Respiratory Health Research
- Nanoplatforms for cancer theranostics
- Neurogenesis and neuroplasticity mechanisms
National University of Singapore
2011-2020
National University Health System
2013-2020
Guangdong Medical College
2011-2019
Safingol is a sphingolipid with promising anticancer potential, which currently in phase I clinical trial. Yet, the underlying mechanisms of its action remain largely unknown. We reported here that safingol-induced primarily accidental necrotic cell death MDA-MB-231 and HT-29 cells, as shown by increase percentage cells stained positive for 7-aminoactinomycin D, collapse mitochondria membrane potential depletion intracellular ATP. Importantly, safingol treatment produced time-...
Rational design of multifunctional and smart drug-delivered nanoplatforms is a promising strategy to achieve simultaneous diagnosis, real-time monitoring, therapy cancers. Herein, highly uniform stable selenium nanoparticles with epidermal growth factor receptor (EGFR) targeting tumor microenvironment-responsive ability (Se-5Fu-Gd-P(Cet/YI-12)) were designed synthesized by using EGFR as the molecule, gadolinium chelate magnetic resonance imaging contrast agent, 5-fluorouracil (5Fu) cetuximab...
Bone marrow mesenchymal stromal cells (BMMSCs) have been used as feeder support for the ex vivo expansion of hematopoietic stem (HSCs) but limitations painful harvest, morbidity, and risk infection to patient. This prompted us explore use human umbilical cord Wharton's jelly MSCs (hWJSCs) its conditioned medium (hWJSC-CM) HSCs in allogeneic autologous settings because hWJSCs can be harvested abundance painlessly, are proliferative, hypoimmunogenic, secrete a variety unique proteins. In...
Responses of human mesenchymal stem cells from bone marrow (hBMSCs) were analyzed under chemical conditions, and then characterization ion channels was evaluated by whole-cell patch clamp.To explore the possibility differentiation marrow-derived into neuron-like in vitro different conditions.The generation has been studied. However, few these studies characterized functional properties differentiated hBMSCs.hBMSCs (Passage 2) expanded cultured vitro. After Passage 5 subcultured, induced...
Several groups have reported that human umbilical cord Wharton's jelly stem cells (hWJSCs) possess unique tumoricidal properties against many cancers. However, the exact mechanisms as to how hWJSCs inhibit tumor growth are not known. Recent evidence suggests exposure of cancer high hydrogen peroxide (H2 O2 ) levels from H2 -releasing drugs causes their death. We therefore explored whether effect on lymphoma was mediated via . first exposed six different molecular weight cut-off (MWCO)...
The transplantation of human umbilical cord blood (UCB) CD34+ cells has been successfully used to treat hematological disorders but one major limitation the low cell numbers available. Mesenchymal stem (MSCs) lying within bone marrow in vivo behave like a scaffold on which interact and proliferate. We therefore evaluated use allogeneic MSCs from UC Wharton's jelly (hWJSCs) as stromal support for ex expansion cells. performed an in-depth evaluation primitiveness, migration, adhesion,...
Mesenchymal stem cells from Wharton's jelly of the human umbilical cord (hWJSCs), and conditioned medium (hWJSC-CM) prepared them, were shown to be tumoricidal on many cancers. However, these effects observed in hWJSCs grown under normoxic conditions 21% oxygen laboratory. Since concentrations cell niche or physiological microenvironment are hypoxic help maintain stemness properties, objective this work was evaluate whether there differences properties hWJSC-CM O2 (normoxic) 5% (hypoxic)...