A5011191590- Pluripotent Stem Cells Research
- Mesenchymal stem cell research
- Reproductive Biology and Fertility
- Renal and related cancers
- Tissue Engineering and Regenerative Medicine
- CRISPR and Genetic Engineering
- Reproductive Health and Technologies
- Assisted Reproductive Technology and Twin Pregnancy
- 3D Printing in Biomedical Research
- Wound Healing and Treatments
- Ovarian function and disorders
- Biomedical Ethics and Regulation
- Prenatal Screening and Diagnostics
- Sperm and Testicular Function
- Neurogenesis and neuroplasticity mechanisms
- Periodontal Regeneration and Treatments
- Dermatologic Treatments and Research
- MicroRNA in disease regulation
- Autophagy in Disease and Therapy
- Hematopoietic Stem Cell Transplantation
- Reproductive System and Pregnancy
- Electrospun Nanofibers in Biomedical Applications
- Congenital heart defects research
- RNA Interference and Gene Delivery
- Neural dynamics and brain function
National University of Singapore
2013-2022
National University Health System
2012-2020
University Health System
2012-2013
National University Hospital
1991-2006
Human embryonic stem (hES) cells are typically derived and serially propagated on inactivated murine fibroblast (MEF) feeders. The use of MEFs other components animal origin in the culture media for hES cell support substantially elevates risk contaminating these lines with infectious agents thereby severely limiting their potential clinical application. We have previously shown that it is possible to derive establish new a xeno-free system using human fetal muscle In this report, we...
Totipotent non-committed inner cell mass (ICM) cells from human blastocysts, if demonstrated to be capable of proliferating in vitro without differentiation, will have several beneficial uses, not only the treatment neurodegenerative and genetic disorders, but also as a model studying events involved embryogenesis genomic manipulation. Nine patients admitted an in-vitro fertilization programme donated 21 spare embryos for this study. All were grown 2-pronuclear until blastocyst stages on...
The human umbilical cord (UC) is an attractive source of mesenchymal stem cells (MSCs) with unique advantages over other MSC sources. They have been isolated from different compartments the UC but there has no rigorous comparison to identify compartment best clinical utility. We compared histology, fresh and cultured cell numbers, morphology, proliferation, viability, stemness characteristics differentiation potential amnion (AM), subamnion (SA), perivascular (PV), Wharton's jelly (WJ) mixed...
Human embryonic stem cells (hESCs) promise to revolutionize reparative medicine through their potential in developing cell replacement therapies for diseases like diabetes and parkinsonism. Most of the existing hESC lines available research, including all National Institutes Health–registered lines, have been derived maintained on mouse fibroblast feeders presence xenoproteins. For future clinical application, many more grown current good manufacturing practice, tissue culture xeno‐free...
Wound healing is a major problem in diabetic patients and current treatments have met with limited success. We evaluated the treatment of excisional wounds using stem cell isolated from human umbilical cord Wharton's jelly (hWJSC) that shares unique properties embryonic adult mesenchymal cells. hWJSCs are non-controversial, available abundance, hypo-immunogenic, non-tumorigenic, differentiate into keratinocytes, secrete important molecules for tissue repair. When skin fibroblasts (CCD)...
Human Wharton's jelly stem cells (hWJSCs) isolated from the human umbilical cord are a unique population of mesenchymal (MSCs) with significant clinical utility. Their broad differentiation potential, high rate proliferation, ready availability discarded cords, and prolonged maintenance stemness properties in culture make them an attractive alternative source MSCs therapeutic value compared bone marrow (hBMMSCs). We aimed to characterize differences gene expression profiles between these two...
Bone marrow mesenchymal stromal cells (BMMSCs) have been used as feeder support for the ex vivo expansion of hematopoietic stem (HSCs) but limitations painful harvest, morbidity, and risk infection to patient. This prompted us explore use human umbilical cord Wharton's jelly MSCs (hWJSCs) its conditioned medium (hWJSC-CM) HSCs in allogeneic autologous settings because hWJSCs can be harvested abundance painlessly, are proliferative, hypoimmunogenic, secrete a variety unique proteins. In...
It has been shown recently that delayed transfers improve implantation rates in assisted reproductive technology programmes. In a prospective study, the pregnancy and safety of outcome were evaluated group patients after transfer day 5 blastocysts with enzymatic treatment zona pellucida. Nineteen women mean age 32.6+/-5.2 years 2.1+/-2.2 repeated attempts had blastocyst number 2.5+/-0.7 embryos replaced per patient. The clinical cycle/transfer rate 53% 33%, respectively. multiple was 40%...