A5054715340- Photodynamic Therapy Research Studies
- Nanoplatforms for cancer theranostics
- bioluminescence and chemiluminescence research
- Ocular Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Nanocomposite Films for Food Packaging
- Antifungal resistance and susceptibility
- Nanoparticles: synthesis and applications
- Electrospun Nanofibers in Biomedical Applications
- Neonatal and Maternal Infections
- Biosensors and Analytical Detection
- Listeria monocytogenes in Food Safety
- Graphene and Nanomaterials Applications
- Wound Healing and Treatments
- Pomegranate: compositions and health benefits
- Bacteriophages and microbial interactions
- Porphyrin and Phthalocyanine Chemistry
- Skin Protection and Aging
- Advanced Nanomaterials in Catalysis
- Advanced Photocatalysis Techniques
- Photoacoustic and Ultrasonic Imaging
- Polymer composites and self-healing
Gdańsk Medical University
2015-2025
University of Gdańsk
2014-2024
A family of N-methylpyrrolidinium fullerene iodide salts has been intensively studied to determine their applicability in antimicrobial photodynamic therapy (APDT). This study examined vitro the efficacy a C60 functionalized with one methylpyrrolidinium group kill upon irradiation white light gram-negative and gram-positive bacteria, as well fungal cells, corresponding mechanism bactericidal action. The studies revealed that high antistaphylococcal could be linked ability photogenerate...
Antimicrobial photodynamic inactivation (aPDI) and antimicrobial blue light (aBL) are considered low-risk treatments for the development of bacterial resistance and/or tolerance due to their multitargeted modes action. In this study, we assessed Staphylococcus aureus these phototreatments. Reference S. USA300 JE2 was subjected 15 cycles both sub-lethal aPDI (employing an exogenously administered photosensitizer (PS), i.e., rose Bengal (RB)) aBL endogenously produced photosensitizing...
Staphylococcus aureus, a major human pathogen causes wide range of disease syndromes. The most dangerous are methicillin-resistant S. aureus (MRSA) strains, resistant not only to all β-lactam antibiotics but also other antimicrobials. An alarming increase in antibiotic resistance spreading among pathogenic bacteria inclines search for alternative therapeutic options, which can be developed easily. Among others, photodynamic inactivation (PDI) is promising option. Photodynamic based on...
Staphylococcus aureus is the most common infectious agent involved in development of skin infections that are associated with antibiotic resistance, such as burn wounds. As drug resistance a growing problem it essential to establish novel antimicrobials. Currently, bacteria successfully controlled by multi-drug therapies. Here we demonstrate secondary metabolites present extract obtained from Drosera binata vitro cultures effective antibacterial agents against S. grown planktonic culture and...
Pseudomonas aeruginosa is among the most common pathogens responsible for both acute and chronic infections of high incidence severity. Additionally, P. resistance to conventional antimicrobials has increased rapidly over past decade. Therefore, it crucial explore new therapeutic options, particularly options that specifically target pathogenic mechanisms this microbe. The ability a bacterium cause disease dependent upon production agents termed 'virulence factors', approaches mitigate these...
In this study, a dual spinneret electrospinning technique was applied to fabricate series of polyurethane (PU) and polyvinyl alcohol-gelatin (PVA/Gel) nanofibrous scaffolds. The study aims enhance the properties PU/PVA-Gel NFs loaded with low dose nanoceria through incorporation cinnamon essential oil (CEO). as-prepared nCeO2 were embedded into PVA/Gel layer, where (CEO) incorporated PU layer. morphology, thermal stability, mechanical properties, chemical composition produced NF mats...
Staphylococcus aureus is a common pathogen responsible for health-care-associated infections as well community acquired ones. It the etiological factor of wide spectrum infections. Therapeutic problems are caused by resistance S. to many antibiotics, specifically methicillin (methicillin-resistant aureus, MRSA). In such cases limited antibiotics may be used and prolonged hospitalization costly. Hence, there an urgent need development alternative antibiotic therapeutics. This work reviews...
Bacterial cell envelope is generally accepted as the primary target for a photo-induced oxidative stress. It plausible that DNA damage occurs during antimicrobial photoinactivation. Here we investigate correlation between and photoinactivation by evaluating level of RecA-based repair system in Staphylococcus aureus. By using exogenous photosensitizers (new methylene blue (NMB), toluidine O (TBO), 5,10,15,20-tetrakis(1-methyl-4-pyridinio)porphyrin tetra(p-toluenesulfonate) (TMPyP), zinc...
The worldwide emergence of extensively drug resistant (XDR) Acinetobacter baumannii has reduced the number antimicrobials that exert high bactericidal activity against this pathogen. This is reason why many scientists are focusing on investigations concerning novel nonantibiotic strategies such as antimicrobial photodynamic inactivation (aPDI) or use blue light (aBL). Therefore, aim current study was to screen for synergies routinely used antibiotics and phototherapies, including both aPDI...
Intending to obtain Punica granatum L. extract (PE)-loaded drug delivery system of better impact and biomedical applicability, the current study reports use crosslinked PVA nanofibers (NFs) as platforms incorporating different amounts biosynthesized PE-CS-gold nanoparticles (PE-CS-Au NPs).PE-conjugated CS-Au NPs) were synthesized via green chemistry approach. The formation PE-CS-Au NPs was confirmed by UV spectroscopy, DLS, SEM STEM. then dispersed into polyvinyl alcohol (PVA) solution at...
It is generally acknowledged that the age of antibiotics could come to an end, due their widespread, and inappropriate use. Particularly for chronic wounds alternatives are being thought. Antimicrobial Photodynamic Therapy (APDT) a potential candidate, while approved some indications, such as periodontitis, sinusitis other niche its use in not established. To further facilitate development APDT we present easy animal model exhibiting key hallmarks wounds, based on full-thickness skin paired...
Pseudomonas aeruginosa is a common causative bacterium of acute and chronic infections that have been responsible for high mortality over the past decade. P. produces many virulence factors such as toxins, enzymes dyes are strongly dependent on quorum sensing (QS) signaling systems. has three major QS systems (las, rhl quinolone signal) regulate expression genes encoding well biofilm production maturation. Antimicrobial blue light (aBL) considered therapeutic option bacterial other benefits,...
Reverse transcription quantitative real-time polymerase chain reaction Q7 (RT‒qPCR) is a commonly used tool for gene expression quantification. Because the qPCR method depends on several variables that can influence analysis process, stably expressed genes should be selected relative studies. To date, there insufficient information selection of appropriate reference antimicrobial photodynamic inactivation (aPDI) and blue light (aBL) treatment. Therefore, purpose present study was to...
Photodynamic therapy (PDT) is based on photosensitizers activated by light of appropriate wavelength. Their activation leads to generation singlet oxygen and free radicals responsible for the cytotoxic effect. The aim this project was compare bactericidal effect PDT using different porphyrin against a methicillin-resistant Staphylococcus aureus strain. Exogenous sensitizers (protoporphyrin IX newly synthesized derivative, protoporphyrin diarginate) induced 3 log10-unit reduction in bacterial...