A5000868226- Neuroscience and Neuropharmacology Research
- Memory and Neural Mechanisms
- Protease and Inhibitor Mechanisms
- Genomics and Chromatin Dynamics
- Genetics and Neurodevelopmental Disorders
- Estrogen and related hormone effects
- Toxoplasma gondii Research Studies
- Epigenetics and DNA Methylation
- Cannabis and Cannabinoid Research
- Enzyme Production and Characterization
- Receptor Mechanisms and Signaling
- Cytokine Signaling Pathways and Interactions
- Neural dynamics and brain function
- Cell Adhesion Molecules Research
- interferon and immune responses
- Retinoids in leukemia and cellular processes
- NF-κB Signaling Pathways
- Child and Animal Learning Development
- Protein Degradation and Inhibitors
- Fatty Acid Research and Health
- Prenatal Substance Exposure Effects
- Cognitive Science and Mapping
- Sleep and Wakefulness Research
- Congenital limb and hand anomalies
- RNA Research and Splicing
University of California, Riverside
2010-2024
University of California, Berkeley
2024
Scripps Research Institute
2004-2005
University of California, San Diego
1998-2004
Howard Hughes Medical Institute
1997-1999
Scripps Whittier Diabetes Institute
1998
Istituti di Ricovero e Cura a Carattere Scientifico
1998
University of Pavia
1998
University of Siena
1998
University of Georgia
1994-1997
Different classes of mammalian transcription factors—nuclear receptors, cyclic adenosine 3′,5′-monophosphate–regulated enhancer binding protein (CREB), and signal transducer activator transcription-1 (STAT-1)—functionally require distinct components the coactivator complex, including CREB-binding (CBP/p300), nuclear receptor coactivators (NCoAs), p300/CBP-associated factor (p/CAF), based on their platform or assembly properties. Retinoic acid receptor, CREB, STAT-1 also different histone...
We report that interferon γ (IFN-γ) inhibits transcription of the macrophage scavenger receptor gene by antagonizing Ras-dependent activities AP-1 and cooperating ets domain factors, apparently as a result competition between AP-1/ets factors activated STAT1 for limiting amounts CBP p300. Consistent with this model, STAT1α interacts directly in cells, microinjection anti-CBP anti-p300 antibodies blocks transcriptional responses to IFN-γ. Cells lacking fail inhibit activity, overexpression...
The immune privileged nature of the CNS can make it vulnerable to chronic and latent infections. Little is known about effects lifelong brain infections, thus inflammation, on neurological health host. Toxoplasma gondii a parasite that infect any mammalian nucleated cell with average worldwide seroprevalence rates 30%. Infection by characterized presence parasitic cysts within neurons in brain, requiring competent system prevent reactivation encephalitis. In immunocompetent individual,...
Both astrocytes in the central nervous system and fibroblasts somatic tissues are not only major sources of extracellular matrix components but also metalloproteinases (MMPs), a family enzymes directly involved breakdown. We have analyzed regulation expression MMPs TIMPs (tissue inhibitors metalloproteinases) human primary stimulated with oncostatin M (OSM) other mediators comparison normal dermal fibroblasts. It was found that OSM induced/enhanced transcription MMP-1 (interstitial...
The neuronal response to a Ca 2+ stimulus is complex process involving direct /calmodulin (CaM) actions as well secondary activation of multiple signaling pathways such cAMP and ERK (extracellular signal-regulated kinase). These signals can act in both the cytoplasm nucleus control gene expression. To dissect role nuclear from cytoplasmic /CaM memory formation, we generated transgenic mice that express dominant inhibitor selectively nuclei forebrain neurons only after animals reach...
CREB-binding proteins (CBP) and p300 are essential transcriptional coactivators for a large number of regulated DNA-binding transcription factors, including CREB, nuclear receptors, STATs. CBP function in part by mediating the assembly multiprotein complexes that contain additional cofactors such as p300/CBP interacting protein (p/CIP), member p160/SRC family coactivators, associated factor p/CAF. In addition to serving molecular scaffolds, each possess intrinsic acetyltransferase activities...
The neural mechanisms underlying the attainment of fear memory accuracy for appropriate discriminative responses to aversive and nonaversive stimuli are unclear. Considerable evidence indicates that coactivator transcription histone acetyltransferase cAMP response element binding protein (CREB) (CBP) is critically required normal function. CBP hypofunction leads severe psychopathological symptoms in human cognitive abnormalities genetic mutant mice with severity dependent on locus...
Aspergillus fumigatus produces a variety of extracellular proteinases that are believed to be virulence factors towards Aspergillus-related lung disease. Among proteinases, the serine proteinase is thought play major virulent role because its widespread production. Nevertheless, evidence direct pulmonary injury caused by A. still lacking. The purpose our work was: (1) provide for pivotal in producing an animal model, and (2) investigate broadness substrate specificity matrix components. To...
Recognition of an object's location in space is supported by hippocampus-dependent recollection. Converging evidence strongly suggests that the interplay between prefrontal cortex and hippocampus critical for spatial memory. Lesion, pharmacological, genetic studies have been successful dissecting role plasticity hippocampal circuit a variety neural processes relevant to memory, including memory objects. However, mechanisms underlying are less well understood. Here, we show acute hypofunction...
Prenatal ethanol exposure (PrEE) produces developmental abnormalities in brain and behavior that often persist into adulthood. We have previously reported abnormal cortical gene expression, disorganized neural circuitry along with deficits sensorimotor function anxiety our CD-1 murine model of fetal alcohol spectrum disorders, or FASD (El Shawa et al., 2013; Abbott 2016). proposed these phenotypes may underlie learning, memory, behavioral humans FASD. Here, we evaluate the impact PrEE on...
Understanding how cortical network dynamics support learning is a challenge. This study investigates the role of local neural mechanisms in prefrontal cortex during contingency judgment (CJL). To better understand brain underlying CJL, we introduce ambiguity into associative after fear acquisition, inducing generalized response to an ambiguous stimulus sharing nontrivial similarities with conditioned stimulus. Real-time recordings at single-neuron resolution from prelimbic (PL) show distinct...
The Semi-alkaline proteinase (Seaprose) from Aspergillus melleus has been tested for its ability to either inactivate or form complexes with three human plasma inhibitors, alpha-2-macroglobulin, alpha-1-antichymotrypsin and alpha-1-proteinase inhibitor. alpha-2-Macroglobulin was found inhibit Seaprose, two mol of enzyme being complexed per However, inhibitor rapidly inactivated by the fungal as a result cleavage inhibitor, primarily at P1-P'1 reactive site. Curiously, Seaprose also be this...