Abstract The coronavirus disease 2019 (COVID-19) first appeared in December and rapidly spread throughout the world. SARS-CoV-2 virus enters host cells by binding to angiotensin-converting enzyme 2 (ACE2). Although much of focus is on respiratory symptoms, recent reports suggest that can cause pregnancy complications such as pre-term birth miscarriages; women with COVID-19 have had maternal vascular malperfusion decidual arteriopathy their placentas. Here, we report ACE2 protein expressed...

Transforming growth factor-beta (TGF-B) plays an important role in embryo implantation; however, TGF-B requires liberation from its inactive latent forms (i.e., large complex [LLC] and small [SLC]) to biologically active monomer or dimer) order act on receptors (TGF-BRs), which turn activate SMAD2/3. Activation of TGF-B1 complexes the uterus is not yet deciphered. We investigated uterine form during implantation, decidualization, delayed implantation. Our study, utilizing nonreducing...

Recurrent pregnancy loss (RPL), characterized by two or more failed clinical pregnancies, poses a significant challenge to reproductive health. In addition embryo quality and endometrial function, proper oviduct function is also essential for successful establishment. Therefore, structural abnormalities inflammation resulting from infection in the may impede transport of embryos endometrium, thereby increasing risk miscarriage. However, our understanding biological processes that preserve...

Recurrent pregnancy loss, characterized by two or more failed clinical pregnancies, poses a significant challenge to reproductive health. In addition embryo quality and endometrial function, proper oviduct function is also essential for successful establishment. Therefore, structural abnormalities inflammation resulting from infection in the may impede transport of embryos endometrium, thereby increasing risk miscarriage. However, our understanding biological processes that preserve...

Abstract Integrin beta8 (ITGB8) is involved in the endometrial receptivity. The blastocyst first interacts with luminal epithelial cells during its implantation; therefore, we have investigated signaling of ITGB8 via FAK and VAV-RAC1 cells. was found elevated at late-pre-receptive (day4, 1600 h) receptive (day5, 0500 stages receptivity period mouse. Integrins downstream molecule has demonstrated an increased expression phosphorylation (Y397) endometrium as well isolated post-receptive...

Pregnancy requires successful implantation of an embryo, which occurs during a restricted period defined as ‘receptivity the endometrium’ and is influenced by ovarian steroids progesterone oestradiol. The role poly(ADP-ribose)polymerase-1 (PARP1) in apoptosis well established. However, it also involved cell differentiation, proliferation tissue remodelling. Previous studies have described presence PARP uterus, but its exact embryo not yet elucidated. Hence, this study, we studied expression...

Integrins (ITGs) are mediators of cell-cell and cell-matrix interactions, which also associated with embryo implantation processes by controlling the interaction blastocyst endometrium. During early pregnancy, ITGbeta8 (ITGB8) has been shown to interact latent transforming growth factor (TGF) beta 1 (TGFB1) at fetomaternal interface. However, precise role ITGB8 in uterus its association not elucidated. Therefore, we attempted ascertain during window process inhibiting function or protein...

Is SARS-CoV-2 receptor, angiotensin-converting enzyme 2 (ACE 2) expressed in the human endometrium during menstrual cycle, and does it participate endometrial decidualization?ACE2 protein is highly stromal cells secretory phase essential for cell decidualization.ACE2 numerous tissues including lungs, heart, intestine, kidneys placenta. ACE2 also receptor by which enters cells.Proliferative (n = 9) 6) biopsies from healthy reproductive-age women primary proliferative were used study.ACE2...

Steroid receptor coactivator-3 (SRC-3; also known as NCOA3 or AIB1) is a member of the multifunctional p160/SRC family coactivators, which includes SRC-1 and SRC-2. Clinical cell-based studies well investigations on mice have demonstrated pivotal roles for each SRC in numerous physiological pathophysiological contexts, underscoring their functional pleiotropy. We previously critical involvement SRC-2 murine embryo implantation human endometrial stromal cell (HESC) decidualization, cellular...

Using an established human primary cell culture model, we previously demonstrated that the promyelocytic leukemia zinc finger (PLZF) transcription factor is a direct target of progesterone receptor (PGR) and essential for progestin-dependent decidualization endometrial stromal cells (HESCs). These in vitro findings were supported by immunohistochemical analysis tissue biopsies, which showed strongest immunoreactivity PLZF detected during (P4)-dominant secretory phase menstrual cycle. While...

Although a benign gynecological disorder, endometriosis displays some pathogenic features of malignancy, such as cell proliferation, migration, invasion, and adaptation to hypoxia. Current treatments include pharmacotherapy and/or surgery, which are limited efficacy often associated with adverse side-effects. Therefore, develop more effective therapies treat this disease, broader understanding the underlying molecular mechanisms that underpin needs be attained. Using immortalized human...

Abstract Although we have shown that steroid receptor coactivator‐2 (SRC‐2), a member of the p160/SRC family transcriptional coregulators, is essential for decidualization both human and murine endometrial stromal cells, SRC‐2's role in earlier stages implantation process not been adequately addressed. Using conditional SRC‐2 knockout mouse ( d/d ) timed natural pregnancy studies, show required embryo attachment adherence to luminal epithelium. Implantation failure associated with persistent...

Summary Early growth response 1 (EGR1) mediates transcriptional programs that are indispensable for cell division, differentiation, and apoptosis in numerous physiologies pathophysiologies. Whole‐body EGR1 knockouts mice ( Egr1 KO ) have advanced our understanding of function an vivo context. To extend the utility mouse to investigate responses a tissue‐ and/or cell‐type‐specific manner, we generated model which exon 2 gene is floxed by CRISPR/Cas9 engineering. The alleles f/f designed...

Recurrent pregnancy loss (RPL), characterized by two or more failed clinical pregnancies, poses a significant challenge to reproductive health. In addition embryo quality and endometrial function, proper oviduct function is also essential for successful establishment. Therefore, structural abnormalities inflammation resulting from infection in the may impede transport of embryos endometrium, thereby increasing risk miscarriage. However, our understanding biological processes that preserve...

Recurrent pregnancy loss (RPL), characterized by two or more failed clinical pregnancies, poses a significant challenge to reproductive health. In addition embryo quality and endometrial function, proper oviduct function is also essential for successful establishment. Therefore, structural abnormalities inflammation resulting from infection in the may impede transport of embryos endometrium, thereby increasing risk miscarriage. However, our understanding biological processes that preserve...