A5101581492- Insect Resistance and Genetics
- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Insect and Pesticide Research
- Insect symbiosis and bacterial influences
- Animal Nutrition and Physiology
- Gut microbiota and health
- Cancer-related Molecular Pathways
- Quinazolinone synthesis and applications
- Melanoma and MAPK Pathways
- Biochemical and Molecular Research
- Plant-Microbe Interactions and Immunity
- Nanoplatforms for cancer theranostics
- Beetle Biology and Toxicology Studies
- Phagocytosis and Immune Regulation
- Silicon Effects in Agriculture
- Chromatography in Natural Products
- Luminescence Properties of Advanced Materials
- Cell death mechanisms and regulation
- Cancer Research and Treatments
- Plant Micronutrient Interactions and Effects
Hubei University
2002-2025
National Health Research Institutes
2007-2024
Liaoning Shihua University
2023
Xian Center for Disease Control and Prevention
2022
Hunan Agricultural University
2019-2022
Shanghai Institute of Ceramics
2021-2022
Chinese Academy of Sciences
2009-2022
University of Chinese Academy of Sciences
2022
Innovation Team (China)
2022
Hunan Vocational College of Safety Technology
2019
TNF and Fas ligand induce apoptosis in tumor cells; however, their severe toxicity toward normal tissues hampers application to cancer therapy. Apo2 (Apo2L, or TRAIL) is a related molecule that triggers cell apoptosis. Apo2L mRNA expressed many tissues, suggesting the may be nontoxic cells. To investigate Apo2L's therapeutic potential, we generated bacteria potently active soluble version of native human protein. Several types were resistant vitro induction by Apo2L. Repeated intravenous...
Summary CRISPR /Cas systems provide bacteria and archaea with molecular immunity against invading phages foreign plasmids. The class 2 type VI effector Cas13a is an RNA ‐targeting that provides protection phages. Here we report the repurposing of /Cas13a to protect potato plants from a eukaryotic virus, Potato virus Y ( PVY ). Transgenic lines expressing Cas13a/sg (small guide ) constructs showed suppressed accumulation disease symptoms. levels viral resistance correlated expression...
The plant root system is important for the uptake of water and nutrients anchoring plants in soil. Lateral roots (LRs) contribute considerably to architecture. Their post-embryonic formation regulated by hormones environmental cues. hormone cytokinin influences LR growth Arabidopsis thaliana on different levels disturbing cell division activity pattern formation. This includes inhibition first formative pericycle founder cells outgrowth young primordia. Mutant analysis revealed that...
The root systems of plants have developed adaptive architectures to exploit soil resources. formation lateral roots (LRs) contributes system architecture. Roots with a lower cytokinin status form LR primordia (LRP) in unusually close proximity, indicating role for the hormone regulating positioning LRs along main axis. Data obtained from cytokinin-synthesis mutants Arabidopsis thaliana combined gene expression analysis indicate that synthesis by IPT5 and LOG4 occurring early during LRP...
Abstract As a non‐invasive treatment modality with high tissue‐penetration depth, ultrasound‐triggered sonodynamic therapy (SDT) has been extensively explored and is regarded as the alternative choice to overcome drawbacks of conventional photo‐triggered therapies. Nevertheless, low quantum yield sonosensitizer, tumor hypoxia, undesirable therapeutic efficiency are still major concerns SDT. It highly challenging but necessary explore SDT‐based synergistic, augmented, noninvasive modalities....
Significance Thrips are a group of piercing-sucking pest insects that do massive damage in agriculture and horticulture. The western flower thrip is particularly notorious has spread all over the world extremely difficult to control. In this work, we have shown upon feeding, thrips take up substantial quantities chloroplast RNA. When expressed from genome long double-stranded RNAs targeted against essential genes, induced potent RNA interference response efficiently killed insects. Our study...
The expression of double-stranded RNAs (dsRNAs) from the plastid genome has been proven to be an effective method for controlling herbivorous pests by targeting essential insect genes. However, there are limitations efficiency plastid-mediated RNA interference (PM-RNAi) due initial damage caused insects and their slow response interference. In this study, we developed transplastomic poplar plants that express dsRNAs β-Actin (dsACT) Srp54k (dsSRP54K) genes Plagiodera versicolora. Feeding...
Expression of double-stranded RNAs in plastids offers great potential for the efficient control chewing insects. However, many insect pests do not consume plant tissue but rather feed on host by sucking sap from vascular system. Whether or plastid-mediated RNA interference (RNAi) can be employed to sap-sucking insects is unknown. Here, we show that five species hemipteran acquire plastid upon feeding plants. We generated both nuclear transgenic and transplastomic tobacco plants expressing...
Abstract Background RNA interference (RNAi) has emerged as a promising technology for insect pest control. Because of the accumulation high levels long double‐stranded RNAs (dsRNAs) in plastids, it was previously shown that expression dsRNAs from plastid genome led to higher mortality some pests with chewing mouthparts than nuclear genome. However, whether plastid‐expressed have effects on phloem sap‐sucking is unknown. In this study, we compared RNAi transgenic and transplastomic plants...
Abstract Plant‐mediated RNA interference (RNAi) has emerged as a promising technology for pest control through expression of double‐stranded RNAs (dsRNAs) targeted against essential insect genes. However, little is known about the underlying molecular mechanisms and whether long dsRNA or short interfering (siRNAs) are effective triggers RNAi response. Here we generated transplastomic nuclear transgenic tobacco plants expressing Helicoverpa armigera ATPaseH gene. We showed that HaATPaseH was...
Summary RNA interference (RNAi) has emerged as an efficient technology for pest control by silencing the essential genes of targeted insects. Owing to its nucleotide sequence‐guided working mechanism, RNAi a high degree species‐specificity without impacts on non‐target organisms. However, plants are inevitably under threat two or more insect pests in nature, species‐specific mode RNAi‐based restricts wide application control. In this study, we artificially designed intermediate dsRNA ( iACT...
Root system architecture (RSA) is developmentally controlled by genetic pathways and their interaction with various environmental cues, in particular soil conditions. One important player shaping RSA the hormone cytokinin, which acts as a negative regulator of root elongation branching. The redundant roles cytokinin metabolism signaling genes Arabidopsis thaliana regulating early stages lateral formation has recently been shown it proposed that this redundancy reflects role mediating...
This study was conducted to investigate the effects of different oligosaccharides on growth performance and intestinal function in broilers.A total 360 1-day-old yellow-feather chickens were randomly divided into 5 groups fed with a basal diet supplemented 50 mg/kg chlortetracycline (ANT), 3 g/kg isomalto-oligosaccharide (IMO), raffinose oligosaccharide (RFO), 30 chitooligosaccharide (COS). The experiment lasted for 56 days, 1-28 days as starter phase 29-56 grower phase.The results showed...
Cytokinins (CKs) can modulate plant immunity to various pathogens, but how CKs are involved in defense responses the necrotrophic pathogen Botrytis cinerea is still unknown. Here, we found that B. infection induced transcriptional changes multiple genes biosynthesis, degradation, and signaling of CKs, as well their contents, pathogen-infected Arabidopsis leaves. Among gene expression CYTOKININ OXIDASE/DEHYDROGENASE 5 ( CKX5 ) was remarkably local infected leaves distant same without...
Plastid transformation for the expression of recombinant proteins and entire metabolic pathways has become a promising tool plant biotechnology. However, large-scale application this technology been hindered by some technical bottlenecks, including lack routine protocols agronomically important crop plants like rice or maize. Currently, there are no standard commercial plastid vectors available scientific community. Construction vector usually requires tedious time-consuming cloning steps....
This study investigated the effects of isomaltooligosaccharide (IMO) and Bacillus in perinatal diets on duration farrowing post-weaning estrus, serum reproductive hormone concentrations, gut microbiota its metabolites sows. Multiparous sows (n = 130) were fed without IMO (control, CON group), or containing only (IMO subtilis (IMOS licheniformis (IMOL B. (IMOSL respectively. The results indicate that estrus was shorter IMOS, IMOL, IMOSL groups, weaning-estrous interval lower IMOL greoup. In...
In our previous work, cytokinin (CK) signaling and biosynthesis were found to be modulated during Arabidopsis defense against infection by the necrotrophic pathogen Botrytis cinerea . Notably, expression level of CYTOKININ OXIDASE/DEHYDROGENASE 5 ( CKX5 ) was significantly induced in B -infected leaves later distant -untreated same plant. To confirm determine how is involved response infection, transcript levels CKX family genes analyzed -inoculated leaves, only remarkably infection....