A5101860792- Pancreatic function and diabetes
- Diabetes Treatment and Management
- Birth, Development, and Health
- Reproductive System and Pregnancy
- Gestational Trophoblastic Disease Studies
- Effects and risks of endocrine disrupting chemicals
- Diet, Metabolism, and Disease
- Reproductive Biology and Fertility
- Cannabis and Cannabinoid Research
- Cell Adhesion Molecules Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Endometrial and Cervical Cancer Treatments
- Endometriosis Research and Treatment
- Diabetes Management and Research
- RNA Research and Splicing
- Adipose Tissue and Metabolism
- Cancer, Lipids, and Metabolism
- Autophagy in Disease and Therapy
- Lipid metabolism and disorders
- Glycosylation and Glycoproteins Research
- Microtubule and mitosis dynamics
- Prenatal Screening and Diagnostics
- Pregnancy and preeclampsia studies
- Cancer, Hypoxia, and Metabolism
- Wnt/β-catenin signaling in development and cancer
Anhui Medical University
2024-2025
Ministry of Education of the People's Republic of China
2024-2025
First Affiliated Hospital of Anhui Medical University
2024-2025
Anhui Provincial Hospital
2024
Lexicon Pharmaceuticals (United States)
2007-2021
Women's Hospital, School of Medicine, Zhejiang University
2010-2020
The First Affiliated Hospital, Sun Yat-sen University
2014-2019
Sun Yat-sen University
2013-2019
Lilly (China)
2018
Fudan University Shanghai Cancer Center
2013
To differentiate the unique and overlapping functions of LFA-1 Mac-1, LFA-1-deficient mice were developed by targeted homologous recombination in embryonic stem cells, neutrophil function was compared vitro vivo with Mac-1-deficient, CD18-deficient, wild-type mice. exhibit leukocytosis but do not develop spontaneous infections, contrast to CD18-deficient After zymosan-activated serum stimulation, neutrophils demonstrated activation, evidenced up-regulation surface did show increased adhesion...
LX4211 [(2S,3R,4R,5S,6R)-2-(4-chloro-3-(4-ethoxybenzyl)phenyl)-6-(methylthio)tetrahydro-2H-pyran-3,4,5-triol], a dual sodium/glucose cotransporter 1 (SGLT1) and SGLT2 inhibitor, is thought to decrease both renal glucose reabsorption by inhibiting intestinal absorption SGLT1. In clinical trials in patients with type 2 diabetes mellitus (T2DM), treatment improved glycemic control while increasing circulating levels of glucagon-like peptide (GLP-1) YY (PYY). To better understand how increases...
Sodium-glucose cotransporter 2 (SGLT2) is the major, and SGLT1 minor, transporter responsible for renal glucose reabsorption. Increasing urinary excretion (UGE) by selectively inhibiting SGLT2 improves glycemic control in diabetic patients. We generated Sglt1 Sglt2 knockout (KO) mice, Sglt1/Sglt2 double-KO (DKO) wild-type (WT) littermates to study their relative determine contributions of UGE. Relative WTs, KOs had improved oral tolerance were resistant streptozotocin-induced diabetes. fed...
Glycosylphosphatidylinositol-anchored HDL-binding protein (GPIHBP1) binds both LPL and chylomicrons, suggesting that GPIHBP1 is a platform for LPL-dependent processing of triglyceride (TG)-rich lipoproteins. Here, we investigated whether affects activity in the absence presence inhibitors angiopoietin-like (ANGPTL)3 ANGPTL4. Like heparin, stabilized but did not activate LPL. ANGPTL4 potently inhibited nonstabilized as well heparin-stabilized GPIHBP1-stabilized ANGPTL4, ANGPTL3 also with less...
Myocardial damage due to reperfusion of ischemic tissue is caused primarily by infiltrating neutrophils. Although leukocyte β 2 integrins (CD18) play a critical role, significant neutrophil emigration persists when CD18 neutralized or absent. This study examined the role 1 integrin (α 4 ) and its endothelial ligand VCAM-1 in CD18-independent migration across cardiac endothelium. In mouse model myocardial ischemia reperfusion, we show that compared with wild-type mice, infiltration efficiency...
Glioma-initiating cells (GIC), which are characterized by their self-renewal capacity and tumorigenicity, were recently identified as a highly tumorigenic subpopulation of glioblastoma multiforme considered responsible for recurrence chemo/radiation resistance. Previously, it was revealed that Wnt signaling activation is critical to the GICs. However, molecular mechanism underlying high expression β-catenin, key transcription factor pathway, remains elusive. In this investigation, determined...
Midkine (MDK) expression is associated with the proliferation of many cancers, including glioma. However, upstream signaling that leads to MDK accumulation remains elusive. This study investigates molecular mechanism induces overexpression in human The Repository for Molecular Brain Neoplasia Data was analyzed identify potential regulators. Expression and specificity protein 1 (SP1) compared glioma specimens. Chromatin immunoprecipitation assay used confirm transcriptional regulation....
The increasing number of people afflicted with diabetes throughout the world is a major health issue. Inhibitors sodium-dependent glucose cotransporters (SGLT) have appeared as viable therapeutics to control blood levels in diabetic patents. Herein we report discovery LX2761, locally acting SGLT1 inhibitor that highly potent vitro and delays intestinal absorption vivo improve glycemic control.
Treatments that lower blood glucose levels and body weight should benefit patients with type 2 diabetes mellitus (T2DM). We developed LX4211 [(2S,3R,4R,5S,6R)-2-(4-chloro-3-(4-ethoxybenzyl)phenyl)-6-(methylthio)tetrahydro-2H-pyran-3,4,5-triol], an orally available small molecule decreases postprandial excursions by inhibiting intestinal sodium/glucose cotransporter 1 (SGLT1) increases urinary excretion (UGE) renal SGLT2. In clinical studies of T2DM, appears to act through dual SGLT1/SGLT2...
Ubiquitin-conjugating enzyme E2C (UBE2C) is characterized as a crucial molecule in cancer cell growth that plays an essential role the development of gliomas, but detailed mechanisms have not been fully elucidated. In this study, we found Forkhead box transcription factor M1 (FoxM1) overexpression increased UBE2C expression, whereas FoxM1 suppression inhibited expression glioma cells. addition, high FoxM1/UBE2C was significantly correlated with poor prognosis glioma. We subsequently...
Noninvasive biomarkers are urgently needed for patients with nonalcoholic steatohepatitis (NASH) to assist in diagnosis, monitoring disease progression and assessing treatment response. Recently several exploratory studies showed that circulating level of microRNA is associated NASH correlated severity. Although these data were encouraging, the application as patient screening stratification need be further assessed under well-controlled conditions. The expression microRNAs profiled...
After creating >4650 knockouts (KOs) of independent mouse genes, we screened them by high-throughput phenotyping and found that cannabinoid receptor 1 (Cnr1) KO mice had the same lean phenotype published others. We asked if our KOs DAG lipase a or b (Dagla Daglb), which catalyze biosynthesis endocannabinoid (EC) 2-Arachidonoylglycerol (2-AG), Napepld, catalyzes EC anandamide, shared Cnr1 mice. Dagla mice, but not Daglb Napepld were among leanest 3651 chow-fed lines screened. In confirmatory...
The use of Bisphenol A (BPA) has been widely restricted due to its adverse health effects. Z (BPZ) is used as an alternative BPA, and humans are exposed BPZ through various routes. Recent studies have shown that exposure adversely affects mouse oocyte meiotic maturation. This study investigates the impact on early embryonic development alongside exploration underlying mechanisms. findings reveal leads a reduction in embryo quality hinders developmental progression. RNA sequencing analysis...
3-Methyl-4-nitrophenol (PNMC) is a prevalent nitrophenolic endocrine disruptor found in pregnant women, with known effects on offspring growth and development. However, its impact fertility remains unexplored. This study investigates the of PNMC exposure during pregnancy underlying mechanisms. Our assessments revealed that reduced number follicles spermatozoa offspring, though it did not affect their quality. In male impaired spermatogenesis by reducing Sertoli cells spermatogonia. female...
P-selectin and intercellular adhesion molecule-1 (ICAM-1) mediate early interaction of neutrophils to coronary endothelial cells myocytes after myocardial ischemia reperfusion. In the present study, we examined physiological consequences genetic deletions ICAM-1 in mice. wild-type mice, 1 h followed by reperfusion, neutrophil influx into area was increased 3 with a peak at 24 decline 72 h. ICAM-1/P-selectin-deficient mice showed significant reduction immunohistochemistry or myeloperoxidase...
Delta-5 desaturase (D5D) and delta-6 (D6D), encoded by fatty acid 1 (FADS1) FADS2 genes, respectively, are enzymes in the synthetic pathways for ω3, ω6, ω9 polyunsaturated acids (PUFAs). Although PUFAs appear to be involved mammalian metabolic pathways, physiologic effect of isolated D5D deficiency on these is unclear. After generating >4,650 knockouts (KOs) independent mouse genes analyzing them our high-throughput phenotypic screen, we found that Fads1 KO mice were among leanest 3,651...
LX2761 is a potent sodium/glucose cotransporter 1 inhibitor restricted to the intestinal lumen after oral administration. Studies presented here evaluated effect of orally administered on glycemic control in preclinical models. In healthy mice and rats treated with LX2761, blood glucose excursions were lower plasma total glucagon-like peptide-1 (GLP-1) levels higher an challenge; these decreased persisted even when challenge occurred 15 hours dosing ad lib-fed mice. Further, treating...
Understanding of the temporal changes hepatic lesions in progression and regression nonalcoholic steatohepatitis (NASH) is vital to elucidation pathogenesis NASH, critical development a strategy for NASH pharmacotherapy. There are challenges studying lesion patients due slow humans, one being requirement multiple biopsies during longitudinal follow-up. Here we studied diet-induced animal model by application or removal pathogenic diet time periods. Male C57BL/6 mice fed Western developed...