A5082843752- Animal Nutrition and Physiology
- Fatty Acid Research and Health
- Lipid metabolism and disorders
- Cholesterol and Lipid Metabolism
- Nutritional Studies and Diet
- Diet and metabolism studies
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Antioxidant Activity and Oxidative Stress
- Lipoproteins and Cardiovascular Health
- Adipokines, Inflammation, and Metabolic Diseases
- Phytochemicals and Antioxidant Activities
- Peroxisome Proliferator-Activated Receptors
- Cancer, Lipids, and Metabolism
- Reproductive Physiology in Livestock
- Liver Disease Diagnosis and Treatment
- Ovarian function and disorders
- Diet, Metabolism, and Disease
- Meat and Animal Product Quality
- Metabolomics and Mass Spectrometry Studies
- Genetic and phenotypic traits in livestock
- Alcohol Consumption and Health Effects
- Muscle metabolism and nutrition
- Atherosclerosis and Cardiovascular Diseases
- Consumer Attitudes and Food Labeling
- Adipose Tissue and Metabolism
Texas A&M University
2015-2025
Bispebjerg Hospital
2015
University of Copenhagen
2015
University of California, Los Angeles
2009-2010
Cornell University
2009-2010
Wake Forest University
2000-2009
Vanderbilt University
2009
Baylor College of Medicine
2009
Mitchell Institute
2008
University of North Carolina at Chapel Hill
2008
GPIHBP1 is an endothelial cell protein that binds lipoprotein lipase (LPL) and chylomicrons. Because deficiency causes chylomicronemia in mice, we sought to determine whether some cases of humans could be attributable defective proteins.Patients with severe hypertriglyceridemia (n=60, plasma triglycerides above the 95th percentile for age gender) were screened mutations GPIHBP1. A homozygous mutation (c.344A>C) changed a highly conserved glutamine at residue 115 proline (p.Q115P) was...
The interaction between fat deposition and inflammation during obesity contributes to the development of non-alcoholic fatty liver disease (NAFLD). present study examined effects palmitoleate, a monounsaturated acid (16∶1n7), on metabolic inflammatory responses, investigated mechanisms by which palmitoleate increases hepatocyte synthase (FAS) expression. Male wild-type C57BL/6J mice were supplemented with subjected assays analyze hepatic steatosis response. Additionally, mouse primary...
Abstract Background Dyslipidaemia and low-grade inflammation are central in atherogenesis linked to overweight physical inactivity. Lifestyle changes important secondary prevention of coronary artery disease (CAD). We compared the effects combined weight loss interval training with alone on fitness, body composition, dyslipidaemia overweight, sedentary participants CAD. Methods Seventy CAD patients, BMI 28–40 kg/m 2 age 45–75 years were randomised (1) 12 weeks’ aerobic (AIT) at 90% peak...
The role of the low density lipoprotein receptor (LDLR) in clearance apo-B48-containing lipoproteins and LDLR-related protein (LRP) removal apo-B100-containing have not been clearly defined. To address these issues, we characterized LDLR-deficient mice homozygous for an "apo-B48-only" allele, "apo-B100-only" or a wild-type apo-B allele (Ldlr-/- Apob48/48, Ldlr-/-Apob100/100, Ldlr-/-Apob+/+, respectively). plasma apo-B48 LDL cholesterol levels were higher Ldlr-/-Apob48/48 than Apob48/48 mice,...
The aim of this study was to investigate the molecular mechanism for changes in proteoglycan binding and LDL receptor affinity on two compositional that have been associated with atherosclerosis: cholesterol enrichment core modification by secretory group IIA phospholipase A2 (sPLA2) surface.Transgenic mice expressing recombinant apolipoprotein (apo) B sPLA2 were generated. Recombinant isolated tested their receptor-binding activity. results show site A (residues 3148-3158) apoB100 becomes...
The purpose of this study was to determine the effects an atherogenic diet on immune function in LDLr(-/-), ApoA-I(-/-) mice.When (DKO), and LDLr(-/-) (SKO) mice were fed diet, DKO had larger peripheral lymph nodes (LNs) spleens compared SKO mice. LNs enriched cholesterol contain expanded populations T, B, dendritic cells, macrophages. Expansion all classes LN cells accompanied by a approximately 1.5-fold increase T cell proliferation activation. Plasma antibodies dsDNA, beta2-glycoprotein...
In mammals, triacylglycerol (TAG) accumulation in nonadipose tissue, termed lipotoxicity, develops with obesity and can provoke insulin resistance, overt diabetes, ovarian dysfunction. Leptin, an adipose tissue hormone, may mediate these effects. Feed-satiated broiler breeder hens manifest lipotoxicity-like symptoms. Changes body organ weights, hepatic plasma TAG, nonesterified fatty acids (NEFA), morphology, egg production response to acute voluntary increases of feed intake were measured 2...
LDL receptor-deficient "apolipoprotein (apo)-B100-only" mice (Ldlr-/-Apob100/100 have elevated cholesterol levels on a chow diet and develop severe aortic atherosclerosis. We hypothesized that both the hypercholesterolemia susceptibility to atherosclerosis could be eliminated by switching off hepatic lipoprotein production.We bred Ldlr-/-Apob100/100 were homozygous for conditional allele Mttp (the gene microsomal triglyceride transfer protein) inducible Mx1-Cre transgene. In these animals,...
Apo-E–deficient apo-B100–only mice (Apoe–/–Apob100/100) and LDL receptor–deficient (Ldlr–/–Apob100/100) have similar total plasma cholesterol levels, but nearly all of the in former animals is packaged VLDL particles, whereas, latter, found smaller particles. We compared apo-B100–containing lipoprotein populations these to determine their relation susceptibility atherosclerosis. The median size particles Apoe–/–Apob100/100 was 53.4 nm versus only 22.1 Ldlr–/–Apob100/100 plasma. levels...
Abstract —The prevention of atherosclerosis by apolipoprotein E (apoE) is generally attributed to the removal plasma lipoprotein remnant particles. We developed transgenic apoE-knockout mice expressing apoE specifically in adrenal gland and found that only 3% wild-type level was sufficient normalize cholesterol levels apoE-deficient mouse. As expected, at correct hypercholesterolemia had almost no cholesteryl ester deposition their aortas. In contrast, nontransgenic siblings significant...
All classes of lipoproteins considered to be atherogenic contain apo-B100 or apo-B48. However, there is a distinct paucity data regarding whether containing apo-B48 differ in their intrinsic ability promote the development atherosclerosis. To address this issue, we compared extent atherosclerosis three groups animals: apo-E-deficient mice (apo-B+/+apo-E-/-) and that synthesize exclusively either (apo-B48/48apo-E-/-) (apo-B100/100apo-E-/-). Mice (n = 25 each group) were fed chow diet for 200...
The consumption of high-fat hamburger enriched with SFA and trans -fatty acids may increase risk factors for coronary vascular disease, whereas MUFA have the opposite effect. Ten mildly hypercholesterolaemic men consumed five, 114 g patties per week two consecutive phases. Participants high-SFA (MUFA:SFA = 0·95; produced from pasture-fed cattle) 5 weeks, their habitual diets 3 weeks then high-MUFA 1·31; grain-fed weeks. These MUFA:SFA ratios were typical ranges observed retail ground beef....
Objective— The risk of atherosclerosis in the setting chylomicronemia has been a topic debate. In this study, we examined susceptibility to Gpihbp1 -deficient mice ( −/− ), which manifest severe as result defective lipolysis. Methods and Results— on chow diet have plasma triglyceride cholesterol levels 2812±209 319±27 mg/dL, respectively. Even though nearly all lipids were contained large lipoproteins (50 135 nm), developed progressive aortic atherosclerosis. other experiments, found that...
Structurally specific very low density lipoprotein (VLDL) particles are presumed to redirect VLDL uptake during yolk deposition. Egg production is an energy intensive process, but excess negatively affects egg production. This study sought determine whether overfeeding changed hen profiles, and if so, changes were related Overfeeding caused 33% of hens stop deposition reabsorb unovulated follicles (involute). control possessed characteristic small particle size (30 nm) composition indicative...
Increases in plasma cholesterol are associated with progressive increases the risk of atherosclerotic cardiovascular disease. In humans is contained primarily apolipoprotein B-based low density lipoprotein (LDL). Cells stop making high-affinity receptor responsible for LDL removal as they become replete; this slows from and elevates LDL. As a result delayed uptake, hypercholesterolemic individuals not only have more but significantly older Oxidative modification enhances their...