A5102023376- Renal and related cancers
- Adipose Tissue and Metabolism
- Urological Disorders and Treatments
- CRISPR and Genetic Engineering
- Congenital heart defects research
- Cholesterol and Lipid Metabolism
- Pediatric Urology and Nephrology Studies
- Drug Transport and Resistance Mechanisms
- Adipokines, Inflammation, and Metabolic Diseases
- Renal cell carcinoma treatment
- Cancer-related molecular mechanisms research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Mitochondrial Function and Pathology
- RNA Research and Splicing
- Genetics and Neurodevelopmental Disorders
- Cancer, Hypoxia, and Metabolism
- Autophagy in Disease and Therapy
- Pancreatic function and diabetes
- Reproductive Biology and Fertility
- Pharmacological Effects and Toxicity Studies
- Cancer-related gene regulation
- Estrogen and related hormone effects
- Sirtuins and Resveratrol in Medicine
- Pregnancy and Medication Impact
- Muscle Physiology and Disorders
Fudan University
2015-2024
State Key Laboratory of Genetic Engineering
2015-2024
Children's Hospital of Fudan University
2016-2024
First Affiliated Hospital of Guangzhou Medical University
2023-2024
State Key Laboratory of Respiratory Disease
2023-2024
Guangzhou Medical University
2023-2024
Huashan Hospital
2024
Huazhong Agricultural University
2021-2023
Czech Academy of Sciences, Institute of Physiology
2023
First Affiliated Hospital of Henan University
2021-2023
Signaling by the Notch surface receptor controls cell fate determination in a broad spectrum of tissues. This signaling is triggered interaction protein with what, so far, have been thought to be transmembrane ligands expressed on adjacent cells. Here biochemical and genetic analyses show that ligand Delta cleaved surface, releasing an extracellular fragment capable binding acting as agonist activity. The ADAM disintegrin metalloprotease Kuzbanian required for this processing event. These...
Oxidative stress contributes to muscle wasting in advanced chronic kidney disease (CKD) patients. Atractylenolide III (ATL-III), the major active constituent of Atractylodes rhizome, has been previously reported function as an antioxidant. This study is aimed at investigating whether ATL-III protective effects against CKD-induced by alleviating oxidative stress. The results showed that levels serum creatinine (SCr), blood urea nitrogen (BUN), and urinary protein significantly decreased...
Dentatorubral-pallidoluysian atrophy (DRPLA) is a progressive neurodegenerative disease caused by polyglutamine expansion within the Atrophin-1 protein. To study mechanism of this and to test potential therapeutic methods, we established Atro-118Q transgenic mice, which express in neurons mutant human protein that contains an expanded stretch 118 glutamines. Consistent with results from previous studies on mice expressed 65 glutamines, exhibited several phenotypes are commonly seen DRPLA...
The breast cancer resistance protein (BCRP) plays an important role in drug disposition, including limiting penetration across the placental barrier. Our goal was to investigate effects of pregnancy on Bcrp1 expression pregnant mice. We examined placenta, kidney, liver, and small intestine at various gestational ages. levels peaked gestation day (gd) 15 gd 10 liver; however, did not change significantly with Immunohistochemistry analysis revealed that cellular localization influenced by...
The plasma concentrations of orally administered anti-human immunodeficiency virus protease inhibitors are significantly reduced during human and mouse pregnancy. We have shown that in the mouse, at gestational day 19, this reduction is due to increased hepatic cytochrome P450 3a (Cyp3a) protein expression activity. In current study, we investigated mechanisms by which Cyp3a activity pregnancy time course change P-glycoprotein (P-gp) various tissues. found transcripts Cyp3a16, Cyp3a41,...
Objective— The risk of atherosclerosis in the setting chylomicronemia has been a topic debate. In this study, we examined susceptibility to Gpihbp1 -deficient mice ( −/− ), which manifest severe as result defective lipolysis. Methods and Results— on chow diet have plasma triglyceride cholesterol levels 2812±209 319±27 mg/dL, respectively. Even though nearly all lipids were contained large lipoproteins (50 135 nm), developed progressive aortic atherosclerosis. other experiments, found that...
Cholesterol homeostasis is maintained through concerted action of the SREBPs and LXRs. Here, we report that RNF145, a previously uncharacterized ER membrane ubiquitin ligase, participates in crosstalk between these critical signaling pathways. RNF145 expression induced response to LXR activation high-cholesterol diet feeding. Transduction into mouse liver inhibits genes involved cholesterol biosynthesis reduces plasma levels. Conversely, acute suppression via shRNA-mediated knockdown, or...
The oncogenic mechanisms of overnutrition, a confirmed independent cancer risk factor, remain poorly understood. Herein, we report that enoyl-CoA hydratase-1 (ECHS1), the enzyme involved in oxidation fatty acids (FAs) and branched-chain amino (BCAAs), senses nutrients promotes mTOR activation apoptotic resistance. Nutrients-promoted acetylation lys
Congenital vertebral malformations (CVMs) are associated with human TBX6 compound inheritance that combines a rare null allele and common hypomorphic at the locus. Our previous in vitro evidence suggested this resulted gene dosage of less than haploinsufficiency (i.e. <50%) as potential mechanism TBX6-associated CVMs. To further investigate pathogenetic model, we ascertained collected 108 Chinese CVM cases found 10 (9.3%) them carried mutations combination variants second allele. For vivo...
Chemerin restricts cold-induced IL-33 production by beige adipocytes, impairing fat’s ability to dissipate excess energy stores.
Abstract Notch pathway has been demonstrated to regulate cardiovascular development. One important step in is the cleavage of receptor, during which an intracellular fragment protein released activate downstream genes. It still uncertain whether Adam10 , mammalian homologue Kuzbanian Drosophila required To further understand physiological function vascular and cardiac development, we generated mice lacking gene primarily endothelial compartment. We found that disruption cells resulted...
Tctn3 belongs to the Tectonic (Tctn) family and is a single-pass membrane protein localized at transition zone of primary cilia as an important component ciliopathy-related complexes. Previous studies showed that mutations in Tctn1 Tctn2, two members tectonic family, have been reported disrupt neural tube development humans mice, but functions brain remain elusive. In this study, knockout (KO) mice were generated by utilizing piggyBac (PB) transposon system. We found KO exhibited abnormal...
Lysosome function is essential to many physiological processes. It has been suggested that deregulation of lysosome could contribute cancer. Through a genetic screen in Drosophila, we have discovered mutations disrupting lysosomal degradation pathway components tumor development and progression. Loss-of-function the Class C vacuolar protein sorting (VPS) gene, deep orange (dor), dramatically promote overgrowth invasion RasV12 cells. Knocking down either two other VPS complex, carnation (car)...
Summary The stable genomic integration and expression of a large transgene is major hurdle in gene therapy. We show that the modified piggyBac (PB) transposon system can be used to introduce 207 kb DNA fragment containing RORγ/γt locus into human cells mice. PB-mediated transgenesis results single copy stably inherited expressed transgene. These indicate PB could serve as an effective high-capacity vector for functional analysis mammalian genome therapy cells.
Zfp462 is a newly identified vertebrate-specific zinc finger protein that contains nearly 2500 amino acids and 23 putative C2H2-type domains. So far, the functions of remain unclear. In our study, we showed expressed predominantly in developing brain, especially cerebral cortex hippocampus regions from embryonic day 7.5 to early postnatal stage. By using piggyBac transposon-generated knockout (KO) mouse model, found KO mice exhibited prenatal lethality with normal neural tube patterning,...