A5075080264- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Lipid metabolism and biosynthesis
- Mitochondrial Function and Pathology
- Immune Cell Function and Interaction
- Cardiovascular Disease and Adiposity
- Exercise and Physiological Responses
- Cancer, Hypoxia, and Metabolism
- Metabolism, Diabetes, and Cancer
- RNA modifications and cancer
- Pancreatic function and diabetes
- Epigenetics and DNA Methylation
- Anaerobic Digestion and Biogas Production
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Peroxisome Proliferator-Activated Receptors
- ATP Synthase and ATPases Research
- Phosphorus and nutrient management
- Calcium Carbonate Crystallization and Inhibition
- IL-33, ST2, and ILC Pathways
- Bone health and treatments
- Genomics and Chromatin Dynamics
- Axon Guidance and Neuronal Signaling
- Microbial metabolism and enzyme function
- TGF-β signaling in diseases
Fudan University
2018-2025
Zhongshan Hospital
2018-2025
The First Affiliated Hospital, Sun Yat-sen University
2025
University of Massachusetts Chan Medical School
2009-2023
State Ethnic Affairs Commission
2023
Minzu University of China
2023
Shanghai Medical College of Fudan University
2005-2022
Hangzhou Dianzi University
2022
Qingdao University
2020
Affiliated Hospital of Qingdao University
2020
Lysine methyltransferase KMT5C catalyzes deposition of trimethylation on histone H4 lysine 20 (H4K20me3), an epigenetic marker usually associated with gene repression and maintenance heterochromatin. is widely expressed in a variety tissues, however, its functional role liver has not been explored. Here, we show Kmt5c fasting- glucagon-induced which regulates hepatic gluconeogenesis. Loss hepatocytes results downregulated gluconeogenic expression compromised glucose output during fasting....
Abstract Extracellular pH is usually low in solid tumors, contrast to the approximately neutral intracellular pH. V-ATPase, which overly functions some cancers with metastatic potential, plays an important role maintaining cytosolic pH, very acidic luminal and extracellular ATP6L, 16 kDa subunit of proton pump can provide hydrophilic transmembrane path. In this study, ATP6L a human hepatocellular carcinoma cell line highly potential (HCCLM3) was knocked down using DNA vector–based small...
Background and Aims NAFLD, characterized by aberrant triglyceride accumulation in liver, affects the metabolic remodeling of hepatic nonhepatic tissues secreting altered hepatokines. Small ubiquitin‐related modifier (SUMO)–specific protease 2 (SENP2) is responsible for de‐SUMOylation target protein, with broad effects on cell growth, signal transduction, developmental processes. However, role SENP2 metabolism remains unclear. Approach Results We found that was most dramatically increased...
Chemerin restricts cold-induced IL-33 production by beige adipocytes, impairing fat’s ability to dissipate excess energy stores.
Brown and beige fat are specialized for energy expenditure by dissipating from glucose fatty acid oxidation as heat. While metabolism have been extensively studied in thermogenic adipose tissues, the involvement of amino acids regulating adaptive thermogenesis remains little studied. Here, we report that asparagine supplementation brown adipocytes drastically upregulated transcriptional program lipogenic gene expression, so asparagine-fed mice showed better cold tolerance. In with...
Great progress has been made in identifying positive regulators that activate adipocyte thermogenesis, but negative regulatory signaling of thermogenesis remains poorly understood. Here, we found cardiotrophin-like cytokine factor 1 (CLCF1) led to loss brown fat identity, which impaired thermogenic capacity. CLCF1 levels decreased during stimulation were considerably increased obesity. Adipocyte-specific transgenic (CLCF1-ATG) mice showed energy expenditure and severe cold intolerance....
Abstract Objective Systemic administration of β‐aminopropionitrile to inhibit lysyl oxidase (Lox) activity improves metabolism, but it exhibits a broad spectrum effects. Clarification the role Lox in adipose tissue metabolism under high‐fat diet (HFD) conditions is needed. Methods Mice with knockout (Lox AKO ) and wild‐type mice were subjected 16‐week HFD regimen. A detailed evaluation encompassing tissue, hepatic function, systemic was conducted. RNA sequencing analysis used unravel...
Peroxisome proliferator-activated receptors (PPARs) are a class of nuclear that play important roles in development and energy metabolism. Whereas PPARδ has been shown to regulate mitochondrial biosynthesis slow-muscle fiber types, its function skeletal muscle progenitors (satellite cells) is unknown. Since constitutive mutation Pparδ leads embryonic lethality, we sought address this question by conditional knockout (cKO) using Myf5-Cre/Pparδflox/flox alleles ablate myogenic progenitor...
Aberrant gluconeogenic gene expression is associated with diabetes, glycogen storage disease, and liver cancer. However, little known how these genes are regulated at the chromatin level. In this study, we investigated in HepG2 cells whether histone demethylation a potential mechanism. We found that knockdown or pharmacological inhibition of demethylase LSD1 causes remarkable transcription activation two genes, FBP1 G6Pase, consequently leads to increased de novo glucose synthesis decreased...
Loss of perivascular adipose tissue (PVAT) impairs endothelial function and enhances atherosclerosis. However, the roles PVAT thermoregulation in vascular inflammation development atherosclerosis remains unclear. Bone morphogenetic protein 4 (BMP4) transforms white adipocyte to beige adipocyte, while promotes a brown-to-white shift inter-scapular brown (BAT). Here, we found that knockdown BMP4 reduced expression adipocyte-characteristic genes increased vitro co-culture system. Ablating...
The transcriptional coactivator PGC-1α is a master regulator of energy metabolism and adaptive thermogenesis in the brown fat cell. short-lived protein, molecular components that control turnover their functional importance are largely unknown. Here we performed luciferase-based overexpression screen identified Ring-finger-containing RNF34, as specific E3 ubiquitin ligase for PGC-1α. RNF34 nuclear protein interacts with ubiquitinates to promote its turnover. Interestingly, binds C-terminal...
Transcriptional co-activator Prdm16 controls brown fat development and white browning, but how this thermogenic function is modulated post-translationally poorly understood. Here, we report that Cbx4, a Polycomb group protein, SUMO E3 ligase for Cbx4-mediated sumoylation of required gene expression. Cbx4 expression enriched in induced adipose tissue by acute cold exposure. Sumoylation at lysine 917 blocks its ubiquitination-mediated degradation, thereby augmenting stability function....
Brown and beige adipocytes dissipate energy in a nonshivering thermogenesis manner, exerting beneficial effects on metabolic homeostasis. CHCHD10 is nuclear-encoded mitochondrial protein involved cristae organization; however, its role thermogenic remains unknown. We identify as novel regulator for adipocyte thermogenesis. dramatically upregulated during activation by PPARγ-PGC1α positively correlated with UCP1 expression adipose tissues from humans mice. generated adipocyte-specific Chchd10...
Mitochondria are the pivot organelles to control metabolism and energy homeostasis. The capacity of mitochondrial metabolic adaptions cold stress is essential for adipocyte thermogenesis. How brown adipocytes keep fitness upon a challenge cold-induced oxidative has not been well characterized. This manuscript shows that IFI27 plays an important role in cristae morphogenesis, keeping intact succinate dehydrogenase (SDH) function active fatty acid oxidation sustain thermogenesis adipocytes....
Browning of subcutaneous white fat (iWAT) involves several reprograming events, but the underlying mechanisms are incompletely understood. Here we show that transcription factor Hlx is selectively expressed in brown adipose tissue (BAT) and iWAT, translationally upregulated by β3-adrenergic signaling-mediated suppression translational inhibitor 4E-BP1. interacts with co-activated Prdm16 to control BAT-selective gene expression mitochondrial biogenesis. heterozygous knockout mice have defects...
Pericardial adipose tissue, which comprises both epicardial tissue (EAT) and paracardial (PAT), has recently been recognized as a novel factor in the pathophysiology of cardiovascular diseases, especially coronary artery disease (CAD). The goal this study was to evaluate differences brown-like characteristic proteome among human EAT, PAT, subcutaneous (SAT) identify candidate molecules causing CAD. Uncoupling protein 1 (UCP-1) other brown-related proteins were highly expressed pericardial...
Abstract The signaling mechanisms underlying adipose thermogenesis have not been fully elucidated. Particularly, the involvement of adipokines that are selectively expressed in brown tissue (BAT) and beige adipocytes remains to be investigated. Here we show a previously uncharacterized adipokine (UPF0687 protein / human C20orf27 homolog) named as Adissp (Adipose-secreted protein) is key regulator for white (WAT) glucose homeostasis. expression adipose-specific highly BAT-enriched, its...
Hepatic gluconeogenesis is required for maintaining blood glucose homeostasis; yet, in diabetes mellitus, this process unrestrained and a major contributor to fasting hyperglycemia. To date, the impacts of chromatin modifying enzymes landscape on are poorly understood. Through catalyzing removal methyl groups from specific lysine residues histone tail, demethylases modulate structure and, hence, gene expression. Here we perform an RNA interference screen against known identify H3 36 (H3K36)...
Significance The thermogenic function of adipocytes relies on the activation program that is coordinately modulated by an array unique transcriptional and epigenetic regulators. Histone methylations represent essential mode regulation in establishment tissue-specific gene expression. However, role H4K20 methylation not completely elucidated. Our paper revealed Kmt5c involved regulating adipose tissues. Adipocyte-specific KO mice demonstrate decreased expression genes are prone to...