A5069873947- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Pancreatic function and diabetes
- Pancreatic and Hepatic Oncology Research
- Single-cell and spatial transcriptomics
- Diabetes and associated disorders
- Cardiovascular Disease and Adiposity
- Lipid metabolism and biosynthesis
- Exercise and Physiological Responses
- Peptidase Inhibition and Analysis
- Stress Responses and Cortisol
- Cholesterol and Lipid Metabolism
- RNA Research and Splicing
- Signaling Pathways in Disease
- Immune Cell Function and Interaction
- Pancreatitis Pathology and Treatment
- Cancer-related molecular mechanisms research
- Regulation of Appetite and Obesity
- Ubiquitin and proteasome pathways
- Apelin-related biomedical research
- IL-33, ST2, and ILC Pathways
- Diet and metabolism studies
- Neuroendocrine regulation and behavior
- Nutrition, Genetics, and Disease
- Mechanisms of cancer metastasis
Icahn School of Medicine at Mount Sinai
2019-2025
University of California, Los Angeles
2016-2022
Howard Hughes Medical Institute
2017-2020
Institute of Nutrition, Metabolism and Diabetes
2019
University of Wisconsin–Madison
2011-2015
University of Wisconsin Carbone Cancer Center
2011
Immune cells are vital constituents of the adipose microenvironment that influence both local and systemic lipid metabolism. Mice lacking IL10 have enhanced thermogenesis, but roles specific cell types in metabolic response to remain be defined. We demonstrate here selective loss receptor α adipocytes recapitulates beneficial effects global deletion, crosstalk between IL10-producing immune is a determinant thermogenesis energy balance. S ingle N uclei A di p ocyte RNA -seq uencing (SNAP-seq)...
ERα gene expression is heritable in humans and prevents obesity mice by improving mitochondrial function via adipocyte Polg1 expression.
Abstract Background Single-cell RNA sequencing (scRNA-seq) provides valuable insights into human islet cell types and their corresponding stable gene expression profiles. However, this approach requires dissociation that complicates its utility in vivo. On the other hand, single-nucleus (snRNA-seq) has compatibility with frozen samples, elimination of dissociation-induced transcriptional stress responses, affords enhanced information from intronic sequences can be leveraged to identify...
Estrogen receptor alpha (ERα), a key driver of growth in the majority breast cancers, contains an unstructured transactivation domain (AF1) its N terminus that is convergence point for factor and hormonal activation. This controlled by phosphorylation, but how phosphorylation impacts AF1 structure function unclear. We found serine 118 (S118) ERα region response to estrogen (agonist), tamoxifen (antagonist), factors results recruitment peptidyl prolyl cis/trans isomerase Pin1. Phosphorylation...
Cholesterol homeostasis is maintained through concerted action of the SREBPs and LXRs. Here, we report that RNF145, a previously uncharacterized ER membrane ubiquitin ligase, participates in crosstalk between these critical signaling pathways. RNF145 expression induced response to LXR activation high-cholesterol diet feeding. Transduction into mouse liver inhibits genes involved cholesterol biosynthesis reduces plasma levels. Conversely, acute suppression via shRNA-mediated knockdown, or...
A highly orchestrated gene expression program establishes the properties that define mature adipocytes, but contribution of posttranscriptional factors to adipocyte phenotype is poorly understood. Here we have shown RNA-binding protein PSPC1, a component paraspeckle complex, promotes adipogenesis in vitro and important for function vivo. Cross-linking immunoprecipitation followed by RNA sequencing revealed PSPC1 binds intronic 3'-untranslated regions number RNAs, including encoding...
Interplay between energy-storing white adipose cells and thermogenic beige adipocytes contributes to obesity insulin resistance. Irrespective of specialized niche, require the activity nuclear receptor PPARγ for proper function. Exposure cold or adrenergic signaling enriches though multiple pathways that act synergistically with PPARγ; however, molecular mechanisms by which licenses tissue preferentially adopt a fate in response dietary cues thermoneutral conditions are not fully elucidated....
Timed feeding drives adipose browning, although the integrative mechanisms for same remain unclear. Here, we show that twice-a-night (TAN) generates biphasic oscillations of circulating insulin and leptin, representing their entrainment by timed feeding. Insulin leptin surges lead to marked cellular, functional, metabolic remodeling subcutaneous white tissue (sWAT), resulting in increased energy expenditure. Single-cell RNA-sequencing (scRNA-seq) analyses flow cytometry demonstrate a role...
Prolonged cold exposure stimulates the recruitment of beige adipocytes within white adipose tissue. Beige depend on mitochondrial oxidative phosphorylation to drive thermogenesis. The transcriptional mechanisms that promote remodeling in tissue during are not well understood. Here we demonstrate coregulator transducin-like enhancer split 3 (TLE3) inhibits gene expression adipocytes. Conditional deletion TLE3 promotes metabolism and increases energy expenditure, thereby improving glucose...
Abstract Background Scientific evidence highlights the influence of biological sex on relationship between stress and metabolic dysfunctions. However, there is limited understanding how diet concurrently contribute to dysregulation in both males females. Our study aimed investigate combined effects high-fat (HFD) induced obesity repeated fear-related behaviors, metabolic, immune, hypothalamic outcomes male female mice. Methods To this, we used a highly reliable rodent behavioral model that...
Obesity has increased to pandemic levels and enhanced understanding of adipose regulation is required for new treatment strategies. Although bone morphogenetic proteins (BMPs) influence adipogenesis, the effect BMP antagonists such as Noggin largely unknown. The aim study was define role Noggin, an extracellular inhibitor, in adipogenesis. We generated adipose-derived progenitor cells a mouse model with adipocyte-specific deletion using AdiponectinCre transgenic mouse, determined phenotype...