A5100335478- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Immune Cell Function and Interaction
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- CRISPR and Genetic Engineering
- Nerve injury and regeneration
- Advanced biosensing and bioanalysis techniques
- Immunotherapy and Immune Responses
- Pluripotent Stem Cells Research
- Cancer Immunotherapy and Biomarkers
- Electrospun Nanofibers in Biomedical Applications
- T-cell and B-cell Immunology
- Transgenic Plants and Applications
- Cytomegalovirus and herpesvirus research
- Viral-associated cancers and disorders
- Insect Resistance and Genetics
- Cancer Cells and Metastasis
- Tissue Engineering and Regenerative Medicine
- MicroRNA in disease regulation
- Biomedical Ethics and Regulation
- Environmental Science and Water Management
- Supramolecular Self-Assembly in Materials
- Animal Genetics and Reproduction
- 3D Printing in Biomedical Research
Qiqihar University
2025
Qiqihar Medical University
2024
Yangtze University
2023-2024
National University of Singapore
2014-2023
Huashan Hospital
2022
Fudan University
2022
Mission Bio (United States)
2022
Institute of Bioengineering and Nanotechnology
2011-2021
GTx (United States)
2017-2020
Broad Institute
2019-2020
Significance Recent data suggest that patients harboring immunologically incompetent tumors fail to respond programmed death 1 (PD-1) blockade. We have developed a mouse tumor model mimics resistance found in human tumors, and we show intratumoral injections of high IFN-inducing CpG oligonucleotide, SD-101, can rapidly lead durable rejection anti–PD-1 nonresponder generate systemic immunity untreated distant-site tumors. The change microenvironment caused by SD-101 leads rapid T-cell...
Abstract In recent years, creating a biodegradable polymer scaffold with an endothelialized surface has become attractive concept for replacement of small‐diameter blood vessels. Toward this end, better understanding the interaction between endothelial cells and substrates is particularly important. Surface roughness biomaterials one important parameters that affect cell behavior. study, human vascular were cultured on electrospun solvent‐cast poly( L ‐lactic acid) different roughness. Cell...
Nanotechnology is an area receiving increasing attention as progress made towards tailoring the morphology of polymeric biomaterial for a variety applications. In present study attempt was to electrospin poly(L-lactide-co-glycolide) biodegradable polymer nanofibres. this process, fibres with diameters down nanometre range are formed by subjecting fluid jet high electric field. The nanofibres were collected on rotating Teflon mandrel and fabricated tubes or conduits, function nerve guidance...
One reason underlying the failure of current chimeric antigen receptor (CAR) immune therapy to treat solid tumors adequately is insufficient tumor infiltration CAR cells. To address issue, we electroporated natural killer (NK) cells with two mRNA constructs encoding chemokine CXCR1 and a targeting tumor-associated NKG2D ligands. The CXCR1-modified NK displayed increased migration toward supernatants in vitro augmented into human vivo subcutaneous intraperitoneal xenograft models. Most...
Natural killer (NK) cells are innate lymphocytes that directly kill tumor and pathogen-infected upon activation by cytokines NK cell receptors (NKRs) without previous sensitization. It is known metabolism affects the differentiation effector functions of immune cells. For instance, interleukin-2 interleukin-15 treatment increases glycolysis oxidative phosphorylation (OXPHOS) in to support their functions. However, little about metabolic reprogramming human NKRs. In this study, we...
// Wei Xia Ang 1, 2, * , Zhendong Li Zhixia Chi 1 Shou-Hui Du Can Chen 3 Johan C.K. Tay Han Chong Toh 4 John E. Connolly 5 Xue Hu Xu 6 Shu Wang 2 Department of Biological Sciences, National University Singapore 117543, Institute Bioengineering and Nanotechnology 138669, Tessa Therapeutics, Pte Ltd., 239351, Division Medical Oncology, Cancer Centre, 169610, Programme in Translational Immunology, for Molecular Cell Biology 138648, Gastrointestinal Surgery, The Third Affiliated Hospital...
Promising progress has been made in adoptive transfer of allogeneic natural killer (NK) cells to treat relapsed or refractory acute myeloid leukemia (AML). In this regard, chimeric antigen receptor (CAR)-modification NK is considered as a compelling approach augment the specificity and cytotoxicity against AML. Using non-viral piggyBac transposon technology human peripheral blood-derived primary cells, we generated CAR-NK target NKG2D ligands demonstrated their vitro activity lysing cancer...
Tiny tubes with fiber architecture were developed by a novel method of fabrication upon introducing some modification to the microbraiding technique, function as nerve guide conduit and feasibility in vivo regeneration was investigated through several these conduits. Poly(L-lactide-co-glycolide) (10:90) polymer fibers being biocompatible biodegradable used for The microbraided conduits (MNGCs) characterized using scanning electron microscopy study surface morphology arrangement. Degradation...
Abstract NKG2D is one of the major activating receptors natural killer (NK) cells and binds to several ligands (NKG2DLs). NKG2DLs are expressed on malignant sensitize them early elimination by cytotoxic lymphocytes. We investigated clinical importance mechanism NKG2DL regulation in breast cancer (BC). Among MICA/B ULBP1/2/3, expression levels BC tissues were inversely associated with Tumor Node Metastasis stage. first found that high MICB, but not MICA, was an independent prognostic factor...
Cytokine-related toxicity associated with the use of highly active chimeric antigen receptor T cells (CAR-T cells) is a significant clinical problem. By fusing natural killer group 2D (NKG2D) ectodomain to 4-1BB and DAP12 cytoplasmic domain containing only one immunoreceptor tyrosine-based activation motif, we have developed 2nd-generation (2nd-Gen) NKG2D CAR for stable expression in human cells. When compared modified commonly used CD3ζ domain, expressing NKG2D-DAP12 stimulated lower level...
Abstract Human embryonic stem (hES) cells as a renewable cell source have great prospective applications in both developmental biology research and regenerative medicine. To realize these potentials, the development of effective safe genetic manipulation methods hES is an obvious demand. We report here that baculoviral vectors were able to transduce efficiently. In transient transduction experiments, recombinant vector equipped with human elongation factor 1-α promoter woodchuck hepatitis...
Abstract Insect baculoviruses are capable of infecting mammalian glial cells in the central nervous system. We investigated current study feasibility using viruses as toxin gene vectors to eliminate malignant glioma brain. first confirmed that were permissive baculovirus infection, with variable transduction efficiencies at 100 viral particles per cell and ranging between 35% 70% seven human rat lines. then developed a recombinant vector accommodating promoter fibrillary acidic protein...
Transcriptional targeting using a tissue-specific cellular promoter is proving to be powerful means for restricting transgene expression in targeted tissues. In the context of cancer suicide gene therapy, this approach may lead cytotoxic effects both and nontarget normal cells. Considering microRNA (miRNA) function post-transcriptional regulation expression, we have developed viral vector platform combining promoter-based transcriptional with miRNA glioma therapy mouse brain. The employed,...
[Yang J, Lam DH, Goh SS, Lee EX, Zhao Y, Tay FC, Chen C, Du S, Balasundaram G, Shahbazi M, Tham CK, Ng WH, Toh HC, Wang S. Tumor tropism of intravenously injected human‐induced pluripotent stem cell‐derived neural cells and their gene therapy application in a metastatic breast cancer model. https://doi.org/10.1002/stem.105110.1002/stem.1051. Stem Cells. (2012) 30: 1021–1029.] This article has been retracted at the request Institute Bioengineering Nanotechnology, Singapore. The author Mr. DH...
Cadmium (Cd) is a non-essential heavy metal that highly toxic to testicle. Selenium (Se) known possess antagonistic effects against Cd toxicity, yet the precise mechanisms through which Se counteracts Cd-induced testicular damage in chickens Nuclear factor erythroid 2-related 2/Heme oxygenase-1 (Nrf2/HO-1) signaling pathway, oxidative stress (OS), apoptosis, and inflammation remained unclear. In present study, experimental model of chicken testis was established by incorporating CdCl2...
Using neural stem cells (NSCs) with tumor tropic migratory capacity to deliver therapeutic genes is an attractive strategy in eliminating metastatic or disseminated tumors. While different methods have been developed isolate generate NSCs, it has not assessed whether induced pluripotent (iPS) cells, a type of that hold great potential for regenerative medicine, can be used as source derivation NSCs tropism. In this study, we conventional lentivirus transduction method derive iPS from primary...