A5024755185- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Animal Genetics and Reproduction
- Reproductive Physiology in Livestock
- Genetic and phenotypic traits in livestock
- Digital Holography and Microscopy
- Periodontal Regeneration and Treatments
- Mesenchymal stem cell research
- Near-Field Optical Microscopy
- Bone Tissue Engineering Materials
- Effects of Environmental Stressors on Livestock
- Seed Germination and Physiology
- Pluripotent Stem Cells Research
- Renal and related cancers
- Facial Trauma and Fracture Management
- Advanced Fluorescence Microscopy Techniques
- Selenium in Biological Systems
- CRISPR and Genetic Engineering
- Vitamin K Research Studies
- T-cell and Retrovirus Studies
- Ovarian function and disorders
- Reproductive Health and Technologies
- Ruminant Nutrition and Digestive Physiology
- Tissue Engineering and Regenerative Medicine
- Vector-Borne Animal Diseases
University of Illinois Urbana-Champaign
2015-2025
Genus (United States)
2021-2025
Genus (United Kingdom)
2024
University of Illinois System
2015-2021
Division of Undergraduate Education
2021
National Research Council
2012-2014
Istituto per il Sistema Produzione Animale in Ambiente Mediterraneo
2012-2014
Federico II University Hospital
2008-2012
University of Naples Federico II
2009
Abstract Multiple scattering limits the contrast in optical imaging of thick specimens. Here, we present gradient light interference microscopy (GLIM) to extract three-dimensional information from both thin and unlabeled GLIM exploits a special case low-coherence interferometry phase specimen, which turn can be used measure cell mass, volume, surface area, their evolutions time. Because it combines multiple intensity images that correspond controlled shifts between two interfering waves, is...
Significance The high incidence of human male factor infertility suggests a need for examining new ways evaluating sperm cells. We present an approach that combines label-free imaging and artificial intelligence to obtain nondestructive markers reproductive outcomes. Our phase-imaging system reveals nanoscale morphological details from unlabeled Deep learning, on the other hand, provides structural specificity map segmenting with accuracy head, midpiece, tail. Using these binary masks...
The COVID-19 pandemic disrupted the world in 2020 by spreading at unprecedented rates and causing tens of thousands fatalities within a few months. number deaths dramatically increased regions where patients need hospital care exceeded availability care. Many experience Acute Respiratory Distress Syndrome (ARDS), condition that can be treated with mechanical ventilation. In response to for ventilators, designed tested an emergency ventilator (EV) control patient's peak inspiratory pressure...
Bone is a plastic tissue with large healing capability. However, extensive bone loss due to disease or trauma requires extreme therapy such as grafting tissue-engineering applications. Presently, the gold standard for repair, but presents serious limitations including donor site morbidity, rejection, and limited regeneration. The use of stem cells appears be means overcome limitations. marrow mesenchymal (BMSC) have been choice thus far cell adipose-derived (ASC) similar immunophenotype,...
Although oocyte cryopreservation has great potentials in the field of reproductive technologies, it still is an open challenge majority domestic animals and little known on biochemical transformation induced by this process different cellular compartments. Raman micro-spectroscopy allows non-invasive evaluation molecular composition cells, based inelastic scattering laser photons vibrating molecules. The aim work was to assess modifications both zona pellucida cytoplasm vitrified/warmed...
A tissue engineering approach to address craniofacial defects requires a biomaterial that balances macro-scale mechanical stiffness and strength with the micron-scale features promote cell expansion biosynthesis. Such criteria are often in opposition, leading suboptimal competence or bioactivity. We report use of multiscale composite integrates polycaprolactone (PCL) reinforcement structure mineralized collagen-glycosaminoglycan scaffold circumvent conventional tradeoffs between mechanics...
Regenerative medicine approaches for massive craniomaxillofacial (CMF) bone defects face challenges associated with the scale of missing bone, need rapid graft-defect integration, and related to inflammation infection. Mineralized collagen scaffolds have been shown promote mesenchymal stem cell osteogenesis due their porous nature material properties, but are mechanically weak, limiting surgical practicality. Previously, these were combined 3D-printed polycaprolactone (PCL) mesh form a...
Characterization of spermatozoon viability is a common test in treating infertility. Recently, it has been shown that label-free, phase-sensitive imaging can provide valuable alternative for this type assay. We employ spatial light interference microscopy (SLIM) to perform high-accuracy single-cell phase and decouple the average thickness refractive index information population. This procedure was enabled by quantitative-phase cells on media two different indices using numerical tool remove...
The objectives of this work were to evaluate whether the sperm penetration speed is correlated in vitro fertility and adapting gamete co-incubation length kinetics bull improves affects sex ratio. In matured oocytes co-incubated with spermatozoa from four different bulls (A-D). At various post-insemination (p.i.) times (4, 8, 12, 16 20 h), samples fixed stained DAPI for nuclei examination, while remaining ones transferred into culture embryo development. blastocysts produced sexed by PCR....
The goal of this study was to characterize sperm populations resulting from three different methods selection used for bovine in vitro fertilization. We compared with discontinuous Percoll gradients, Swim-Up, and electro-channel. Spatial light interference microscopy (SLIM) evaluate the morphology spermatozoa computer-assisted semen analysis (CASA) motility behavior sperm. Using these two technologies, we analyzed morphometric parameters kinetic (motility) patterns frozen-thawed Holstein...
Reproductive biotechnologies in dromedary camel (Camelus dromedarius) are less developed than other livestock species. The vitro maturation (IVM) technology is a fundamental step for embryo production (IVP), and its optimization could represent way to increase the success rate of IVP. aim present study was investigate bioenergy/oxidative status oocytes before after IVM by confocal microscopy 3D imaging. Oocytes were retrieved slicing ovaries collected at local slaughterhouses. Recovered...
In the past few years, new technologies have arisen that enable higher efficiency of gene editing. With increase ease using editing technologies, it is important to consider best method for transferring genetic material livestock animals. Microinjection a technique has proven be effective in mice but less efficient large Over variety methods been used cloning as well transfer including; nuclear transfer, sperm mediated (SMGT), and liposome-mediated DNA transfer. This review looks at...
Our objective is to evaluate the effects of feeding rumen-protected Met (RPM) throughout transition period and early lactation on lipid profile preimplantation embryos endometrial tissue Holstein cows. Treatments consisted a total mixed ration with top-dressed RPM (Smartamine
Abstract Assisted reproduction is one of the significant tools to treat human infertility. Morphological assessment primary method determine sperm and embryo viability during in vitro fertilization cycles. It has advantage being a quick, convenient, inexpensive means assessment. However, visual observation limited predictive value for early morphology. led many search other imaging assess reproductive potential given embryo. The limitations apply both humans animals. One recent innovation...
In vitro embryo production and transfer (ET) in buffaloes has been developed for decades. However, most studies are focused on the donor or laboratory improvements, there is a lack of reports regarding recipients. Therefore, our aim was to investigate factors associated pregnancy (P/ET), loss (PL), calving rates buffalo The studied were season, recipient parity, synchronization protocol, CL diameter, asynchrony between recipient, day estrous cycle, (fresh vs. vitrified), development, stage....
Objective: This study investigated the effect of two different follicle stimulating hormone (FSH) preparations (diluted/dynamised and diluted) on in vitro development steroid production (estradiol, progesterone testosterone) isolated porcine preantral after culture. Methods: Secondary follicles were cultured Alpha Minimum Essential Medium (α-MEM+) supplemented with grain ethanol (AL – 0.2%, v/v), diluted/dynamised FSH (rFSH 6cH 0.05 fg/mL) or diluted-only (1.5 ng/mL) for 4 days. Follicle was...