A5043405732- Extracellular vesicles in disease
- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Cell Adhesion Molecules Research
- Immune cells in cancer
- Phagocytosis and Immune Regulation
- CAR-T cell therapy research
- Gene Regulatory Network Analysis
- Cancer Cells and Metastasis
- Immunotherapy and Immune Responses
- Cell Image Analysis Techniques
- Cancer Genomics and Diagnostics
- Ferroptosis and cancer prognosis
- Blood transfusion and management
- Nanowire Synthesis and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer Research and Treatments
- Cancer Immunotherapy and Biomarkers
- Bioinformatics and Genomic Networks
- Microfluidic and Bio-sensing Technologies
- Caveolin-1 and cellular processes
- Cancer, Hypoxia, and Metabolism
- Neonatal Respiratory Health Research
- Nanofabrication and Lithography Techniques
- Angiogenesis and VEGF in Cancer
Johns Hopkins University
2022-2024
Chengdu University
2020-2024
University of Baltimore
2023
Physical Sciences (United States)
2022
Cornell University
2014
Tissue stiffness is a critical prognostic factor in breast cancer and associated with metastatic progression. Here we show an alternative complementary hypothesis of tumor progression whereby physiologic matrix affects the quantity protein cargo small extracellular vesicles (EV) produced by cells, which turn aid cell dissemination. Primary patient tissue released cells on matrices that model human tumors (25 kPa; stiff EVs) feature increased adhesion molecule presentation (ITGα2β1, ITGα6β4,...
ABSTRACT Chimeric antigen receptor (CAR) T cells express antigen-specific synthetic receptors, which upon binding to cancer cells, elicit cell anti-tumor responses. CAR therapy has enjoyed success in the clinic for hematological indications, giving rise decade-long remissions some cases. However, patients with solid tumors not seen similar success. Solid constitute 90% of adult human cancers, representing an enormous unmet clinical need. Current approaches do solve central problem limited...
Cell motility plays an essential role in many biological processes as cells move and interact within their local microenvironments. Current methods for quantifying cell typically involve tracking individual over time, but the results are often presented averaged values across populations. While informative, these ensemble approaches have limitations assessing cellular heterogeneity identifying generalizable patterns of single-cell behaviors, at baseline response to perturbations. In this...
Enrichment of tumor-associated macrophages (TAMΦs) in the tumor microenvironment correlates with worse clinical outcomes triple-negative breast cancer (TNBC) patients, prompting development therapies to inhibit TAMΦ infiltration. However, lackluster efficacy CCL2-based chemotaxis blockade trials suggests that a new understanding monocyte/macrophage infiltration may be necessary. Here we demonstrate random migration, and not only chemotaxis, drives macrophage We identified tumor- associated...
In acute lung injury, destruction of the endothelial glycocalyx leads to vessel permeabilization and contributes pulmonary edema inflammation. Heparan sulfate, which accounts for >70% glycosaminoglycans in glycocalyx, plays a crucial physiological anti-inflammatory role. To treat it is explored whether two-step vivo bioorthogonal chemistry strategy can covalently link intravenously administered heparan sulfate vascular endothelium damaged glycocalyx. First, fusogenic liposomes (EBP-Tz-FLs)...
Abstract Immune cell-mediated killing of cancer cells in a solid tumor is prefaced by multi-step infiltration cascade invasion, directed migration, and cytotoxic activities. In particular, immune must invade migrate through series different extracellular matrix (ECM) boundaries domains before reaching their target cells. These events are central challenge to the clinical success CAR T against tumors. The current standard vitro cell assays measure cytotoxicity an obstacle-free,...
A special microenvironment called the "pre-metastatic niche" is thought to help primary tumor cells migrate new tissues and invade them, in part because normal barrier function of vascular endothelium compromised. While itself can promote creation such niches by secreting pro-metastatic factors, underlying molecular mechanisms are still poorly understood. Here, we show that injection tumor-secreted factors from B16F10 melanoma or 4T1 breast cancer into healthy mice induce destruction...
ABSTRACT Cell motility plays an essential role in many biological processes as cells move and interact within their local microenvironments. Current methods for quantifying cell typically involve tracking individual over time, but the results are often presented averaged values across populations. While informative, these ensemble approaches have limitations assessing cellular heterogeneity identifying generalizable patterns of single-cell behaviors, at baseline response to perturbations. In...
<p>Stiff EVs promote cancer cell migration and dissemination. <b>A,</b> Schematic of 2-day GFP+ zebrafish embryo model system used to test the ability MDA-MB-231 (red, filled triangles) disperse cells (blue, open triangles). Scale bar, 100 µm. <b>B,</b> Full body images embryos at time 0 24 hours postinjection cells. Representative images. <b>C,</b> Enlarged disseminated (white) (red). <b>D,</b> Percentage injected with dissemination head...
<p>Soft EVs transform the phenotype of resident lung fibroblasts. <b>A</b> and <b>B,</b> Gene expression fold change in <i>ACTA2</i>, <i>CCN2</i>, <i>COL1A1</i>, <i>IL6</i>, <i>KGF</i>, <i>MMP1</i>, <i>VIM</i>, <i>VEGFA</i>, <i>S100A4, S100A6, S100A10, S100A11, S100A12, S100A13, S100A14, S100A16</i> assessed by qRT-PCR IMR90 human fibroblasts exposed to...
<div>Abstract<p>Tissue stiffness is a critical prognostic factor in breast cancer and associated with metastatic progression. Here we show an alternative complementary hypothesis of tumor progression whereby physiologic matrix affects the quantity protein cargo small extracellular vesicles (EV) produced by cells, which turn aid cell dissemination. Primary patient tissue released cells on matrices that model human tumors (25 kPa; stiff EVs) feature increased adhesion molecule...
<p>Table S1 - Primers for qRT-PCR analysis</p>
<p>Isolation and characterization of EVs</p>
<div>Abstract<p>Tissue stiffness is a critical prognostic factor in breast cancer and associated with metastatic progression. Here we show an alternative complementary hypothesis of tumor progression whereby physiologic matrix affects the quantity protein cargo small extracellular vesicles (EV) produced by cells, which turn aid cell dissemination. Primary patient tissue released cells on matrices that model human tumors (25 kPa; stiff EVs) feature increased adhesion molecule...
<p>Table S1 - Primers for qRT-PCR analysis</p>
<p>Additional Methods</p>
<p>Master protein list of identified proteins present in TMT analysis.</p>
<p>Stiff EVs show increased biodistribution and retention <i>in vivo.</i><b>A,</b> Schematic of EV isolation staining prior to tail-vein injection 10 µg in nude mice. NIR imaging (<b>B</b>) mean SNR (<b>C</b>) MDA-MB-231 vesicle dorsal, left, ventral, right sides (mean ± SEM). Signal intensity is arbitrary units (a.u.). Three mice 0.5 kPa condition, four 25 condition; one-way ANOVA. (<b>D</b>) (<b>E</b>) the lungs,...
<p>Comparison of protein cargo for plastic, stiff and soft EVs</p>
<p>Comparison of protein cargo for plastic, stiff and soft EVs</p>
<p>Stiff EVs promote cancer cell migration and dissemination. <b>A,</b> Schematic of 2-day GFP+ zebrafish embryo model system used to test the ability MDA-MB-231 (red, filled triangles) disperse cells (blue, open triangles). Scale bar, 100 µm. <b>B,</b> Full body images embryos at time 0 24 hours postinjection cells. Representative images. <b>C,</b> Enlarged disseminated (white) (red). <b>D,</b> Percentage injected with dissemination head...