A5021196840- Stress Responses and Cortisol
- Adipose Tissue and Metabolism
- Birth, Development, and Health
- Pain Mechanisms and Treatments
- Gastrointestinal motility and disorders
- Ion Channels and Receptors
- Urinary Bladder and Prostate Research
- Neuroendocrine regulation and behavior
- Diet and metabolism studies
- Pediatric Pain Management Techniques
- Dermatology and Skin Diseases
- Anesthesia and Neurotoxicity Research
- Regulation of Appetite and Obesity
- Nerve injury and regeneration
- Endometriosis Research and Treatment
- Biochemical effects in animals
- Menstrual Health and Disorders
- Exercise and Physiological Responses
- Hormonal Regulation and Hypertension
- Pelvic floor disorders treatments
- Endometrial and Cervical Cancer Treatments
- Helicobacter pylori-related gastroenterology studies
- Sexual function and dysfunction studies
- Viral gastroenteritis research and epidemiology
- Musculoskeletal pain and rehabilitation
University of Kansas Medical Center
2015-2024
Institute of Cell Biology and Neurobiology
2019
University of Kansas
2003-2017
University of Pittsburgh Medical Center
2004-2013
University of Pittsburgh
2005-2010
Zero to Three
2008
Dichotomizing afferents are individual dorsal root ganglion (DRG) neurons that innervate two distinct structures thereby providing a form of afferent convergence may be involved in pelvic organ cross-sensitization. To determine the distribution dichotomizing supplying distal colon and bladder Sprague-Dawley rat C57Bl/6 mouse, we performed concurrent retrograde labeling urinary using cholera toxin subunit B (CTB) fluorescent conjugates. Animals were perfused 4-5 days after sub-serosal...
The nerve growth factor (NGF) and glial cell line-derived neurotrophic (GDNF) families of factors regulate the sensitivity sensory neurons. ion channels transient receptor potential vanilloid 1 (TRPV1) channel, subfamily A, member (TRPA1), are necessary for development inflammatory hypersensitivity functionally potentiated by factors. We have shown previously that inflamed skin exhibits rapid increases in artemin mRNA with slower, smaller NGF mRNA. Here, using mice, we show that, colon, both...
Abstract Visceral pain is a prevalent clinical problem and one of the most common ailments for which patients seek medical attention. Recent studies have described many physiological properties visceral afferents, but not much known regarding their anatomical characteristics. To determine spinal distribution neurochemical phenotype colonic afferents in rodents, Alexa Fluor‐conjugated cholera toxin‐β (CTB) was injected subserosally into proximal distal portions descending colon Sprague Dawley...
Summary Exercise reverses mechanical and visceral hyperalgesia in a prediabetic mouse model. Hyperalgesia is associated with increased nerve growth factor the periphery, which exercise normalizes. Recent research suggests that can be effective reducing pain animals humans neuropathic pain. To investigate mechanisms may improve prediabetes, C57Bl/6 mice were fed either standard chow or high-fat diet for 12 weeks provided access to running wheels (exercised) without (sedentary). The induced...
Abstract Dysfunction of primary afferents innervating the pancreas has been shown to contribute development painful symptoms during acute and chronic pancreatitis. To investigate distribution neurochemical phenotype pancreatic afferents, Alexa Fluor‐conjugated cholera toxin B (CTB) was injected into head (CTB‐488) tail (CTB‐555) adult male mice label neurons retrogradely in both dorsal root ganglia (DRG) nodose (NG). The NG DRG (T5–T13) were processed for fluorescent immunohistochemistry...
Changes in primary sensory neurons are likely to contribute the emergence of chronic visceral pain. An important step understanding pain is development comprehensive phenotypes that combines functional and anatomical properties for these neurons. We developed a novel ex vivo physiology preparation mice allows intracellular recording from colon during distension, presence absence pharmacologic agents. This also recovery functionally characterized afferents histochemical analysis. Recordings...
Inflammation or pain during neonatal development can result in long-term structural and functional alterations of nociceptive pathways, ultimately altering perception adulthood. We have developed a mouse model colon irritation (NCI) to investigate the plasticity processing within viscerosensory system. Mouse pups received an intracolonic administration 2% mustard oil (MO) on postnatal days 8 10. Distal colons were processed at subsequent timepoints for myeloperoxidase (MPO) activity growth...
Exposure to stress early in life has been associated with adult-onset comorbidities such as chronic pain, metabolic dysregulation, obesity, and inactivity. We have established an early-life model using neonatal maternal separation (NMS) mice, which displays evidence of increased body weight adiposity, widespread mechanical allodynia, hypothalamic-pituitary-adrenal axis dysregulation male mice. Early-life consumption a Western-style diet contribute the development obesity; however, relatively...
Mammalian pain-related sensory neurons are derived from TrkA lineage located in the dorsal root ganglion. These project to peripheral targets throughout body, which can be divided into superficial and deep tissues. Here, we find that transcription factor Runx1 is required for development of many epidermis-projecting neurons. Accordingly, knockout leads selective loss innervation epidermis, whereas tissue two types pain unaffected. Within these cutaneous neurons, suppresses a large molecular...
Patients with interstitial cystitis/painful bladder syndrome (IC/PBS) commonly suffer from widespread pain and mood disorder, which has been attributed to improper functioning of the hypothalamic-pituitary-adrenal (HPA) axis. Voluntary exercise shown improve HPA axis function, therefore we are determining whether voluntary wheel running can attenuate urological dysfunction following neonatal maternal separation (NMS) in female mice.Mice underwent NMS for 3 h/day postnatal Day 1-21, were...