A5049157751- CAR-T cell therapy research
- Gene Regulatory Network Analysis
- Immune Cell Function and Interaction
- Historical Art and Culture Studies
- Evolution and Genetic Dynamics
- Historical Economic and Social Studies
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Shakespeare, Adaptation, and Literary Criticism
- Diffusion and Search Dynamics
- Scottish History and National Identity
- Immunotherapy and Immune Responses
- Poetry Analysis and Criticism
- Architecture, Modernity, and Design
- Australian History and Society
- Monoclonal and Polyclonal Antibodies Research
- Molecular Communication and Nanonetworks
- Comics and Graphic Narratives
- Travel Writing and Literature
- Art, Politics, and Modernism
- Insect and Arachnid Ecology and Behavior
- Visual Culture and Art Theory
- Irish and British Studies
- Digital Games and Media
- Single-cell and spatial transcriptomics
University of California, San Francisco
2018-2022
University of San Francisco
2022
Harvard University Press
2022
San Francisco Art Institute
2022
Howard Hughes Medical Institute
2018-2019
Yale University
2014-2018
Virginia Commonwealth University
2001-2016
Rice University
2008
Chimeric antigen receptor (CAR) T cells are ineffective against solid tumors with immunosuppressive microenvironments. To overcome suppression, we engineered circuits in which tumor-specific synNotch receptors locally induce production of the cytokine IL-2. These potently enhance CAR cell infiltration and clearance immune-excluded tumors, without systemic toxicity. The most effective IL-2 induction circuit acts an autocrine (TCR)- or CAR-independent manner, bypassing suppression mechanisms...
Bacterial chemotaxis systems are as diverse the environments that bacteria inhabit, but how much environmental variation can cells tolerate with a single system? Diversification of system could serve an alternative, or even evolutionary stepping-stone, to switching between multiple systems. We hypothesized mutations in gene regulation lead heritable control chemotactic diversity. By simulating foraging and colonization E. coli using single-cell model, we found different selected for...
Abstract Biological functions are typically performed by groups of cells that express predominantly the same genes, yet display a continuum phenotypes. While it is known how one genotype can generate such non‐genetic diversity, remains unclear different phenotypes contribute to performance biological function at population level. We developed microfluidic device simultaneously measure phenotype and chemotactic tens thousands individual, freely swimming Escherichia coli as they climbed...
Understanding how stochastic molecular fluctuations affect cell behavior requires the quantification of both and protein numbers in same cells. Here, we combine automated microscopy with situ hydrogel polymerization to measure single-cell expression after tracking swimming behavior. We characterized distribution non-genetic phenotypic diversity Escherichia coli motility, which affects exploration. By expressing fluorescently tagged chemotaxis proteins (CheR CheB) at different levels,...
Acute myeloid leukemia (AML) is an aggressive disease with a poor prognosis (5-year survival rate of 30.5% in the United States). Designing cell therapies to target AML challenging because no single tumor-associated antigen (TAA) highly expressed on all cancer subpopulations. Furthermore, TAAs are also healthy cells, leading toxicity risk. To address these targeting challenges, we engineer natural killer (NK) cells multi-input gene circuit consisting chimeric receptors (CARs) controlled by...
A new method for protein surface functionalization was developed that utilizes DNA-conjugated artificial polypeptides to capture recombinant target proteins from the solution phase and direct their deposition onto DNA-functionalized matrices. Protein is accomplished through coiled-coil association of an engineered pair heterodimeric leucine zippers. Incorporating half zipper complex directly into labeling these polymers with ssDNA enables polypeptide conjugates form intermediate linkages...
Abstract/Summary Acute myeloid leukemia (AML) is a hematopoietic malignancy characterized by antigen heterogeneity and poor prognosis. A potential therapeutic approach to address this targeting multiple surface antigens prevent escape relapse. Chimeric receptor (CAR) T cells are an adoptive cell therapy that have demonstrated remarkable clinical success in the treatment of B malignancies, many efforts underway adapt them malignancies. To tackle AML, logically through “A OR B” gated CAR...
Abstract CAR-based cell therapies have revolutionized cancer treatment, however, applications beyond targeting lineage antigens are challenging due to expression of targeted in healthy cells posing a risk for on-target off-tissue toxicities. This presents an opportunity leverage synthetic biological logic gated gene circuits, such as NOT gate, expand targets therapies. We constructed first-in-class gate CAR-NK protect from CAR-mediated cytotoxicity. An inhibitory CAR (iCAR) recognizes safety...
Review Article| September 01 2003 Christina Rossetti and Illustration: A Publishing History NICHOLAS FRANKEL Virginia Commonwealth University Search for other works by this author on: This Site PubMed Google Scholar Nineteenth-Century Literature (2003) 58 (2): 275–280. https://doi.org/10.1525/ncl.2003.58.2.275 Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Share Facebook Twitter LinkedIn MailTo Tools Get Permissions Cite Citation FRANKEL; History. 1 2003;...
In his recent books The Aesthetics of Environment and Living in the Landscape philosopher Arnold Berleant has argued that arts—and aesthetic more generally—have an important role to play generating "environmental" consciousness through their explicit appeal senses general tendency enliven us tissue world. arts "embody continuity with other human domains," writes Berleant, helping grasp, deliberate engagement conscious body, "the perceiver is aspect perceived and, conversely, person...
Bacterial chemotaxis systems are as diverse the environments that bacteria inhabit, but how much environmental variation can cells tolerate with a single system? Diversification of system could serve an alternative, or even evolutionary stepping-stone, to switching between multiple systems. We hypothesized mutations in gene regulation lead heritable control chemotactic diversity. By simulating foraging and colonization E. coli using single-cell model, we found different selected for...
Abstract Understanding how stochastic molecular fluctuations affect cell behavior requires the quantification of both and protein numbers in same cells. Here, we combine automated microscopy with situ hydrogel polymerization to measure single-cell expression after tracking swimming behavior. We characterized distribution non-genetic phenotypic diversity Escherichia coli motility, which affects exploration. By expressing fluorescently tagged chemotaxis proteins (CheR CheB) at different...
Wilde se rendit compte dès le début de sa carrière que genre du portrait reposait sur une dichotomie entre la représentation des aspects intimes vie d’un individu d’une part et celle personnage public, d’autre part. Mais peu après criminalisation « outrages aux bonnes mœurs » en 1885 liaison avec Robert Ross 1886, il prit conscience fait constituait également structure imaginaire propice à vies caractérisées par désirs illicites, l’on ne pouvait satisfaire secrètement, loin regard société....
In 1841 John Murray published a sumptuously ornamented edition of Gibson Lockhart’s Ancient Spanish Ballads. Murray’s new edition, printed using the very latest bookmaking technologies and pitched at readership newly accustomed to paying exorbitant prices for book ornaments illustrations, was radically different from first ballads, which had appeared without accompanying ornament in 1823. Illustrated by leading illustrators day decorated throughout multiple colors architect Owen Jones (who...
Meredith’s poetics, explicitly articulated and theorized in his poem “The Woods of Westermain,” are diametrically opposed to those New Criticism. Always conscious its own incompleteness, poetry calls out reader as an active co-producer meaning. For this reason, like Robert Browning’s, work has been haunted by critical accusations obscurity incoherence since earliest publication. This essay argues that dissolves the customary imagined boundaries between poem, text – perceiving subject...