A5101399817- Retinal Imaging and Analysis
- Retinal Diseases and Treatments
- Glaucoma and retinal disorders
- Retinal and Optic Conditions
- Optical Coherence Tomography Applications
- Corneal surgery and disorders
- Traumatic Brain Injury and Neurovascular Disturbances
- Ocular Surface and Contact Lens
- Retinal Development and Disorders
- Diabetes Treatment and Management
- Blood Pressure and Hypertension Studies
- Traffic and Road Safety
- Injury Epidemiology and Prevention
- Neuropeptides and Animal Physiology
- Cardiovascular and Diving-Related Complications
- Immune Cell Function and Interaction
- Pediatric health and respiratory diseases
- Paraquat toxicity studies and treatments
- Diet, Metabolism, and Disease
- Viral Infections and Immunology Research
- Reproductive System and Pregnancy
- Mosquito-borne diseases and control
- Vascular Malformations Diagnosis and Treatment
- Viral gastroenteritis research and epidemiology
- Retinal and Macular Surgery
The University of Western Australia
2017-2024
Lions Eye Institute
2017-2024
Jiangsu Agri-animal Husbandry Vocational College
2024
Jiangsu Hengrui Medicine (China)
2022
Guizhou Center for Disease Control and Prevention
2019
Commonwealth Scientific and Industrial Research Organisation
2017
Purpose: To use structural criteria to reconcile the three-dimensional organization and connectivity of parafoveal microvasculature. Methods: The microvasculature was perfused labeled in 16 normal human donor eyes for lectin, alpha smooth muscle actin, filamentous actin. Established gathered using confocal microscopy, including vessel diameter, endothelial cell morphology, presence/density cells, were used differentiate arteries, arterioles, capillaries, venules, veins. Three-dimensional...
Microaneurysms are biomarkers of microvascular injury in diabetic retinopathy (DR). Impaired retinal capillary perfusion is a critical pathogenic mechanism the development abnormalities. Targeting fundamental molecular disturbances resulting from nonperfusion, such as increased vascular endothelial growth factor expression, does not always reverse anatomic complications DR, suggesting that other mechanisms independent also play role. We stratify effects inflammation, and pericyte loss on...
The escalating incidence of diabetes mellitus has amplified the global impact diabetic retinopathy. There are known structural and functional changes in retina that precede fundus photography abnormalities which currently used to diagnose clinical Understanding these subclinical alterations is important for effective disease management. Histology high-resolution imaging reveal entire neurovascular unit, comprised retinal vasculature, neurons glial cells, affected disease. Early...
To use high-resolution histology to define the associations between microaneurysms, capillary diameter and density alterations in diabetic retinopathy (DR).Quantitative comparisons of microaneurysm number, were performed eight human donor eyes with nonproliferative DR six age- eccentricity-matched normal after retinal vascular perfusion labelling. The parafovea, 3-mm, 6-mm, 9-mm eccentricities analyzed microvascular defined.Mean was reduced all retina regions group (P = 0.013)....
To define the three-dimensional topologic organization of human peripapillary capillary plexuses in order to better understand hemodynamic characteristics this retinal circulation.The microvasculature was perfusion labeled five normal donor eyes, and optical stacks were collected from regions immediately superior, temporal, inferior, nasal optic disk by using confocal scanning laser microscopy. The spatial location morphometric compared. Three-dimensional visualization strategies used...
Purpose: To characterize macular blood flow connectivity in vivo using high-resolution optical coherence tomography (HighRes OCT). Methods: Cross-sectional, observational study. Dense (6-µm interscan distance) perifoveal HighRes OCT raster scans were performed on healthy participants. mitigate the limitations of projection-resolved OCT-angiography, and structural data used to observe vascular structures superficial complex (SVC) deep complex. Vascular segmentation rendering Imaris 9.5...
Robust detection of hemorrhages (HMs) in color fundus image is important an automatic diabetic retinopathy grading system. Detection the that are close to or connected with retinal blood vessels was found be challenge. However, most methods didn't put research on it, even some them mentioned this issue. In paper, we proposed a novel hemorrhage method based rule-based and machine learning methods. We focused improvement vessels, besides detecting independent regions. A preliminary test for HM...
Purpose: To investigate differences in alpha smooth muscle actin (αSMA) expression and parafoveal blood flow pathways diabetic retinopathy (DR). Methods: Human donor eyes from healthy subjects (n = 8), patients with diabetes but no DR (DR–; n 7), clinical (DR+; 13) were perfusion labeled antibodies targeting αSMA, lectin, collagen IV, filamentous actin. High-resolution confocal scanning laser microscopy was used to quantify αSMA staining capillary density the circulation. Quantitative...
Oxidative stress increases the apoptosis of intestinal epithelial cells and impairs cell renewal, which further promotes barrier dysfunction even death. Extensive evidence supports that resveratrol apigenin have antioxidant, anti-inflammatory, antiproliferative properties. Here, we investigated ability these two compounds to alleviate diquat-induced jejunal oxidative morphological injury, using duck as a model, well effects on induced by H2O2 in immortalized (IDECs). Ducks were randomly...
Abstract An adequate blood supply to meet the energy demands is essential for any tissue, particularly high demand tissues such as retina. A critical question is: How dynamic match between neuronal and achieved? We present a quantitative assessment of temporal spatial variations in perfusion macular capillary network 10 healthy human subjects using non-invasive label-free imaging technique. The based on calculation coefficient variation (CoV) signal from arterioles, venules capillaries...
We employ optical coherence tomography (OCT) and microscopy (OCM) to study conjunctival lymphatics in porcine eyes ex vivo. This is a precursor the development of vivo imaging collecting for potentially guiding monitoring glaucoma filtration surgery. OCT scans at 1300 nm higher‐resolution OCM 785 reveal lymphatic vessels via their transparency. Equivalent signal characteristics are also observed from blood largely free (and devoid flow) conjunctiva. In our lymphangiography, vessel networks...
The purpose of this study was to use three-dimensional confocal microscopy quantify the spatial patterns capillary network alterations in nonproliferative diabetic retinopathy (NPDR).The retinal microvasculature perfusion-labelled seven normal human donor eyes and six age-matched with NPDR. peripapillary microcirculation studied using scanning laser microscopy. Capillary density diameters radial plexus (RPCP), superficial (SCP), intermediate (ICP), deep (DCP) were quantified compared....
Purpose: We studied the topographic distribution of contractile protein in different orders human macular microvasculature to further understanding sites for capillary blood flow regulation. Methods: Nine donor eyes from eight donors were cannulated at central retinal artery and perfusion labeled alpha smooth muscle actin (αSMA) filamentous (F-actin). Confocal images collected macula region, viewed, projected, converted a 255 grayscale measurements. The mean intensity was measured...
Spaceflight associated neuro-ocular syndrome (SANS) is common amongst astronauts on long duration space missions and with signs consistent elevated cerebrospinal fluid (CSF) pressure. Additionally, CSF pressure has been found to be in a significant proportion of whom lumbar puncture was performed after successful mission completion. We have developed retinal photoplethysmographic technique measure vein pulsation amplitudes. This enabled the development non-invasive measurement apparatus....
Purpose To explore the potential relationship between optic disc haemorrhage, venous pulsation pressure (VPP), ocular perfusion pressures and visual field change in glaucomatous glaucoma suspect eyes. Materials methods This prospective observational study examined 155 open angle or eyes from 78 patients over 5 years. Patients were followed with 3 monthly non-mydriatic photographs, 6 standard automated perimetry annual ophthalmodynamometry. The number of haemorrhages each hemidisc was counted...
Background/aims To explore the relationship between focal lamina defect (LD) size and optic disc haemorrhages (DH) in glaucomatous eyes. Methods Radial B-scan images at 15° intervals obtained using enhanced depth imaging (EDI) spectral-domain optical coherence tomography (OCT) were performed on a group of subjects previously assessed for DH every 3 months over period 5 years. EDI-OCT scans presence cribrosa defects by single observer. Results 119 eyes from 62 (44 females, 18 males) analysed....