A5110222567- Cancer, Lipids, and Metabolism
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Psoriasis: Treatment and Pathogenesis
- Genetic and Kidney Cyst Diseases
- Cytokine Signaling Pathways and Interactions
- Bone Metabolism and Diseases
- Mollusks and Parasites Studies
- DNA Repair Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Diet, Metabolism, and Disease
- Cancer, Hypoxia, and Metabolism
- Telomeres, Telomerase, and Senescence
- Cannabis and Cannabinoid Research
- Cell death mechanisms and regulation
- Nicotinic Acetylcholine Receptors Study
- RNA modifications and cancer
- Orthodontics and Dentofacial Orthopedics
- Pain Management and Placebo Effect
- Bladder and Urothelial Cancer Treatments
- Aldose Reductase and Taurine
- Immune Cell Function and Interaction
- Genomics, phytochemicals, and oxidative stress
- Facial Trauma and Fracture Management
- Autoimmune and Inflammatory Disorders Research
National Defense Medical Center
2019-2025
National Taiwan University
2009-2025
Academia Sinica
2025
Chang Gung University
2024
Taichung Veterans General Hospital
2024
National Yang Ming Chiao Tung University
2023
National Sun Yat-sen University
2023
The University of Melbourne
2014
Peter Doherty Institute
2014
Soochow University
2010
To follow the fate of CD8+ T cells responsive to Plasmodium berghei ANKA (PbA) infection, we generated an MHC I-restricted TCR transgenic mouse line against this pathogen. from line, termed PbT-I cells, were able respond blood-stage infection by PbA and two other rodent malaria species, P. yoelii XNL chabaudi AS. These also sporozoites protect mice liver-stage infection. Examination requirements for priming after intravenous administration irradiated sporozoites, effective vaccination...
Benzyl isothiocyanate (BITC) is a dietary chemopreventive agent that inhibits the growth of various human cancer cells by causing apoptotic cell death. In this study, we demonstrate BITC not only induces apoptosis but also autophagy in hormone-sensitive (Rv1) and -refractory (PC3) prostate cells. BITC-treated cells, induction was detected monitoring processing an marker protein, microtubule-associated protein 1 light chain 3 (LC3), aggregation LC3 into granular structures formation acidic...
Interleukin 26 (IL-26) is a new member of the IL-10 family that highly expressed in rheumatoid arthritis (RA). However, functions IL-26 produced by macrophages RA have not been elucidated. In present work, we evaluated effects and mechanisms on M1 M2 macrophage differentiation. Human or mouse cells were treated with lipopolysaccharides (LPS), interferon gamma (IFNγ), IL-4 alone concurrently to monitor subtypes. The expression level genes was reverse transcription polymerase chain reaction...
Bisphosphonates are potent inhibitors of bone resorption. In vitro studies show that zolendronic acid inhibits prostate cancer cell growth by activating apoptosis. We investigated whether also autophagy (type II programmed death).We the induction in PC-3, DU-145, LNCaP and CRW22Rv1 lines upon treatment. LC3-II protein formation was detected Western blot. incorporation into autophagosomes immunofluorescence staining. Acidic organelle acridine orange Rescue experiments using an apoptosis...
ABSTRACT Habituation to painful stimuli reflects an endogenous pain alleviation mechanism, and reduced habituation has been demonstrated in many chronic conditions. In ethology, animals exhibit fear responses while habituating repeated threatening stimuli. It remains unclear whether humans involves a reduction mechanism. fMRI experiment, we investigated pain‐related brain before after the development of induced by repetitive stimulation healthy adults. another behavioral examined emotional...
Budding yeast telomerase is mainly activated by Tel1/Mec1 (yeast ATM/ATR) on Cdc13 from late S to G2 phase of the cell cycle. Here, we demonstrated that telomerase-recruitment domain also phosphorylated Cdk1 at same cycle stage as Tel1/Mec1-dependent regulation. Phosphor-specific gel analysis phosphorylates residues 308 and 336 Cdc13. The residue T308 critical for efficient Mec1-mediated S306 phosphorylation in vitro. Phenotypic vivo revealed mutations S/TP motifs caused delay telomere...
Aims: Osteoarthritis (OA) represents the prevailing form of degenerative joint pathology. Recent investigations have revealed a heightened expression interleukin 26 (IL-26) in various inflammatory arthritic conditions, including OA. However, specific impacts and functions IL-26 on osteoblasts (OBs) within context OA remain inadequately elucidated. This study aims to clarify effects underlying mechanisms by examining its influence isolated from patients murine osteoblast cell line. Methods:...
Bladder cancer (BC) is a malignant tumor of the urinary system with high mortality and recurrence rates. Proteasome subunit type 4 (PSMB4) highly expressed has been identified as having oncogenic properties in variety types. This study aimed to explore effect PSMB4 knockdown on survival, migration, angiogenesis human bladder cells different degrees malignancy. We analyzed effects endothelial microenvironment. was patients low- high-grade urothelial carcinoma. Inhibition reduced protein...
IL-26 has been shown to have high expression in RA. However, the effects of on bone destruction RA not evaluated. The aim this study was investigate and mechanisms RANK ligand (RANKL)-induced osteoclastogenesis.We treated cells with RANKL-induced oseteoclastogenesis monitor osteoclast formation by tartrate-resistant acid phosphatase (TRAP) staining. Osteoclast activity assessed pit assay F-actin ring formation. mechanism inhibition studied biochemical analyses such as RT-PCR,...
The immune synapse, a highly organized structure formed at the interface between T lymphocytes and antigen-presenting cells (APCs), is essential for cell activation adaptive response. It has been shown that this shares similarities with primary cilium, sensory organelle in eukaryotic cells, although roles of ciliary proteins on synapse remain elusive. Here, we find inositol polyphosphate-5-phosphatase E (INPP5E), cilium-enriched protein responsible regulating phosphoinositide localization,...
Telomere maintenance is required for chromosome stability, and telomeres are typically replicated by the action of telomerase. In both mammalian tumor yeast cells that lack telomerase, maintained an alternative recombination mechanism. Here we demonstrated budding Saccharomyces cerevisiae type I survivors derived from telomerase-deficient were hypersensitive to DNA damaging agents. Assays track telomere lengths drug sensitivity spore colonies suggested a correlation between shortening...
Glioblastoma is believed to be one of the most aggressive brain tumors in world. ONX-0914 (PR957) a selective inhibitor proteasome subunit beta type-8 (PSMB8). Previous studies have shown that inhibiting PSMB8 expression glioblastoma reduces tumor progression. Therefore, this study aimed determine whether has antitumor effects on human glioblastoma. The results indicated treatment inhibited survival LN229, GBM8401, and U87MG cells. Cell cycle analysis showed caused cell arrest at G1 phase...
In order to satisfy the requirement of applications at 5G or next-generation communication, mmWave antenna becomes an important part systems. this work, stacked patch is adopted in 8-layer packaging with a thickness 0.92 mm. The double-layer parasitic elements are proposed on package (AiP) achieve broad band. This AiP operates dual polarization and band which includes low from 24.2 GHz 29.6 high 36.6 40.5 GHz.
You have accessJournal of UrologyProstate Cancer: Basic Research1 Apr 2011744 ZOLEDRONIC ACID INDUCES AUTOPHAGIC CELL DEATH IN HUMAN PROSTATE CANCER CELLS Yi-Chia Lin, Ji-Fan Te-Fu Tsai, Kuang-Yu Chou, Hung-En Chen, Yi-Hsuan and Thomas I.S. Hwang LinYi-Chia Lin Taipei, Taiwan More articles by this author , LinJi-Fan TsaiTe-Fu Tsai ChouKuang-Yu Chou ChenHung-En Chen LinYi-Hsuan HwangThomas View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.1713AboutPDF ToolsAdd to...
Interleukin-1α (IL-1α) is a potent cytokine that plays role in inflammatory arthritis and bone loss. Decoy receptor 3 (DCR3) an immune modulator of monocytes macrophages. The aim this study was to investigate the mechanism DCR3 IL-1α-induced osteoclastogenesis.We treated murine macrophages with during activator nuclear factor kappa Β ligand- (RANKL-) plus osteoclastogenesis monitor osteoclast formation by tartrate-resistant acid phosphatase (TRAP) staining. Osteoclast activity assessed using...
Angiostrongylus cantonensis is a significant foodborne zoonotic parasite that causes severe neuropathological damage and symptoms in humans. Excretory-secretory products (ESPs) play pivotal role elucidating host-parasite interactions can aid penetrating host defensive barriers helminths. Recently, secreted microRNAs have become important research targets for parasite-host communication. In this study, we determined the expression function of novel from A. L5 ESPs evaluated effect target on...