ABSTRACT Progress in metabolomic analysis now allows the evaluation of food quality. This study aims to identify metabolites meat from livestock using a approach. Using gas chromatography–mass spectrometry (GC/MS), many were reproducibly detected meats, and distinct differences between species (cattle, pigs, chickens) indicated. A comparison tissues types (muscle, intramuscular fat, intermuscular fat) marbled beef Japanese Black cattle revealed that most are abundant muscle tissue. Several...

Beef from Japanese Black cattle (Japanese Wagyu) is renowned for its flavor characteristics. To clarify the key metabolites contributing to this rich and sweet aroma of beef, an omics analysis combined with GC-olfactometry (GC-O) metabolomics gas chromatography–mass spectrometry (GC-MS) were applied. GC-O identified 39 odor-active odorants volatile fraction boiled beef distilled by solvent-assisted evaporation. Eight predicted contribute Wagyu compared between Holstein using a stable isotope...

Aroma is an essential factor for meat quality. The of Japanese Black cattle exhibits fine marbling and a rich sweet aroma with characteristic lactone composition. mechanism formation associated beef has not been elucidated. In this study, we examined the precursors γ-hexalactone, indicator identified underlying γ-hexalactone production. A low-temperature vacuum system was used to prepare tallow from Holstein cattle. odor components were using headspace-gas chromatography. analysis revealed...

Abstract Diabetic nephropathy (DN) is one of vascular complications diabetes and caused by abnormal protein kinase C activation as a result increased diacylglycerol (DG) production in diabetic hyperglycaemia. Diacylglycerol (DGK) converts DG into phosphatidic acid. Therefore, it expected that the DGK would ameliorate DN. Indeed, has been reported vitamin E (VtE) ameliorates DN rat activating DGK, we recently VtE specifically activates DGKα isoform vitro . However, whether involved...

Japanese Black cattle (Japanese Wagyu) have a unique phenotype in which ectopic intramuscular fat accumulates skeletal muscle, producing finely marbled beef. However, the mechanism of formation remains unclear. To investigate key genes involved accumulation, we comprehensively analyzed mRNA levels subcutaneous and tissues using RNA sequence (RNA-seq) analysis, detected 27,606 genes. We identified eight genes, namely carboxypeptidase E, tenascin C, transgelin, collagen type IV alpha 5...

Abstract Significant efforts have been made to ameliorate diabetic nephropathy (DN) by inhibiting protein kinase C. However, these not successful in human trials, suggesting that novel therapeutic strategies are required. Thus far, it has reported green tea polyphenol epigallocatechin gallate (EGCg) improved albuminuria DN a trial. Our previous study revealed activation of diacylglycerol α (DGKα) plays crucial role the amelioration and EGCg activates DGKα. Here, we investigated whether how...

The meat from Japanese Black cattle (Japanese Wagyu) is finely marbled and exhibits a rich sweet aroma known as Wagyu beef aroma. To clarify the key metabolites involved in aroma, we analyzed correlation between lactone lipid composition cattle. Using gas chromatography-olfactometry, identified 39 characteristic odorants of intermuscular fat. Seven lactones considered to be were quantified compared area fat using stable isotope dilution assay. Among them, γ-hexalactone was only whose level...

Abstract Diacylglycerol kinase γ (DGKγ) regulates protein C (PKC) activity by converting DG to phosphatidic acid (PA). DGKγ directly interacts with PKCγ and is phosphorylated PKCγ, resulting in the upregulation of lipid activity. PKC dysfunction impairs motor coordination, indicating that regulation important for coordination. are abundantly expressed cerebellar Purkinje cells. However, physiological role has not been elucidated. Therefore, we developed knock-out (KO) mice tested their In KO...

Journal Article Effect of Paratropomyosin on the Increase in Sarcomere Length Rigor-Shortened Skeletal Muscles Get access Minoru Yamanoue, Yamanoue Faculty Agriculture Department Animal Science,Hokkaido UniversityKita-ku, Sapporo, Hokkaido 060 Search for other works by this author on: Oxford Academic PubMed Google Scholar Koui Takahashi 3 3To whom correspondence should be addressed. The Biochemistry, Volume 103, Issue 5, May 1988, Pages 843–847,...

Abstract Background Diacylglycerol kinase (DGK) is a key enzyme that regulates diacylglycerol (DG) turnover and involved in variety of physiological functions. The isoform DGKθ has unique domain structure the sole member type V DGK. To reveal spatial temporal expression we performed immunohistochemical staining on paraffin sections mouse embryos. Results At an early stage development (E10.5 11.5), was prominently detected brain, spinal cord, dorsal root ganglion, limb bud, also moderately...

A protein component which is released from skeletal-muscle myofibrils on the treatment with Ca2+ at concentrations above 10−5 M and modifies actin-myosin interaction was purified by a method involving column chromatography Sephadex G-200 DEAE-cellulose in succession. Although this resembles tropomyosin some physicochemical properties, it has same chain weight of 34,000 as α-component SDS-polyacrylamide gel electrophoresis, differed not only amino acid composition but also prolonging clearing...

PCR法による肉種鑑別を行う目的で,既知のウシ,ブタ,ニワトリのミトコンドリアD-loop近傍の塩基配列の相同性を調査し,それらの3種を対象に3セットのプライマーBFとBR,PF(BFと同一のもの)とPR,CFとCRを合成した.3種食肉と各種肉製品から抽出したDNAを鋳型とし,94°C-2分間,66-68°C-2分間,72°C-3分間で35回の繰り返し反応でPCR増幅を行った.増幅されたDNAは2%アガロースゲル電気泳動を用いて分析した.その結果,設計通りにウシ,ブタ,ニワトリに特異的なDNA断片(518,321,400bp)が増幅された.この方法を生肉,加熱肉及びいくつかの肉製品について適用したところ,試料に含まれていると推定される種特異的なDNA断片が検出された.さらに,ウシ,ブタ,ニワトリの3セットプライマーを混合することで,3種類の食肉の存在を一度の分析で鑑定できた.羊肉を牛肉と誤認する可能性は否定された.混合DNAを用いてウシ,ブタ,ニワトリの複数種のDNAの検出限度について検討した結果,混合サンプル中のそれぞれの検出限界は0.1%,0.001%,1%で,いずれの場合で...

Abstract Diabetic nephropathy (DN) is a diabetic vascular complication, and abnormal protein kinase C (PKC) activation from increased diacylglycerol (DG) production in hyperglycemia one of the causes DN. Diacylglycerol (DGK) converts DG into phosphatidic acid. In other words, DGK can attenuate PKC activity by reducing amount DG. Recently, we reported that intraperitoneally administered d-α-tocopherol (vitamin E, αToc) induces an amelioration DN vivo through DGKα prevention podocyte loss....

International trading markets of meat require the animal's age information to prevent cross-contamination ineligible products. Individual livestock is either evaluated from physiological features or verified by breeding history. However, it remains impossible perform verification on when a suspicion error occurred in importing country. To investigate an age-related protein skeletal muscle livestock, we compared expression among chicken pectoralis major different ages. Results indicated that...

We have used an enzymatic technique to determine the weakening effect of paratropomyosin, a new myofibrillar protein, on rigor linkages formed between actin and myosin, clarify distinct function as that tropomyosin. Paratropomyosin inhibited Mg-ATPase activity enhanced K-ATPase reconstituted actomyosin stoichiometrically, its maximal binding was estimated occur at molar ratio 1: 12.5. also by 49% 126%, while tropomyosin had no these ATPases. These results indicate paratropomyosin is able...

黒毛和種腿肉の熟成中における筋漿蛋白質の変化を詳細に追究する目的で,筋漿画分を細胞質画分として分画し,種々の筋漿蛋白質含量の変化およびSDS-ポリアクリドアミドゲル電気泳動(PAGE)像の変化を指標として調査した.屠殺後0,7,14,21,28日間熟成した試料の種々の筋漿蛋白質含量を測定した結果,ミトコンドリア画分は熟成28日において熟成7,14,21日試料より有意(P<0.05)に低い値が得られた.さらに,リソソーム画分においては熟成7日,14日試料に比べて21日と28日で有意(P<0.05)に低い値が認められた.サイトゾル画分のSDS-PAGE像の結果から,ホスホリラーゼb,クレアチンキナーゼ,ホスホグリセロアルデヒド脱水素酵素は熟成に伴い顕著に減少した.一方,乳酸脱水素酵素,ホスホグリセロムターゼ,トリオースホスホイソメラーゼに相当する蛋白質は熟成7日,14日に比べて熟成21日と28日試料で増加した.

Diacylglycerol kinase γ (DGKγ) is a lipid to convert diacylglycerol (DG) phosphatidic acid (PA) and indirectly regulates protein C (PKCγ) activity. We previously reported that the basal PKCγ upregulation impairs cerebellar long-term depression (LTD) in conventional DGKγ knockout (KO) mice. However, precise mechanism impaired LTD by upregulated has not been clearly understood. Therefore, we first produced Purkinje cell-specific KO (tm1d) mice investigate specific function of cells confirmed...

Senescence-accelerated mouse prone 8 (SAMP8) is an animal model of age-related central nervous system (CNS) disorders. Although SAMP8 shows deficits in learning, memory, and emotion, its motor coordination has not been clarified. We have recently reported that DGKγ-regulated PKCγ activity important for cerebellar coordination. However, involvement the functional correlation between kinases dyscoordination still remains unknown. Therefore, we investigated DGKγ dyscoordination. 6 weeks old...