A5068311388- Neuroendocrine regulation and behavior
- Regulation of Appetite and Obesity
- Cancer Cells and Metastasis
- Cancer Research and Treatments
- Virus-based gene therapy research
- Genomics and Chromatin Dynamics
- Reproductive System and Pregnancy
- TGF-β signaling in diseases
- Antenna Design and Optimization
- GaN-based semiconductor devices and materials
- Stress Responses and Cortisol
- Sleep and Wakefulness Research
- Developmental Biology and Gene Regulation
- Nanoplatforms for cancer theranostics
- Estrogen and related hormone effects
- Semiconductor materials and devices
- Anodic Oxide Films and Nanostructures
- Alzheimer's disease research and treatments
- Lipid Membrane Structure and Behavior
- Epigenetics and DNA Methylation
- Satellite Communication Systems
- S100 Proteins and Annexins
- Antenna Design and Analysis
- Neuroscience of respiration and sleep
- Adipose Tissue and Metabolism
Teikyo University
2018-2025
Okayama University of Science
2021-2022
Kawasaki Medical School
2014-2021
Kyoto University
1983-2021
Kyoto Katsura Hospital
2021
The University of Tokyo
1991-2017
RIKEN BioResource Research Center
2002-2016
University of Fukui
2000-2016
Daido University
2013
Saiseikai Nakatsu Hospital
2011-2012
Chromatin reorganization is essential for transcriptional control by sequence-specific transcription factors. However, the molecular link between and chromatin reconfiguration remains unclear. By colocalization of nuclear ecdysone receptor (EcR) on ecdysone-induced puff in salivary gland, Drosophila DEK (dDEK) was genetically identified as a coactivator EcR both insect cells intact flies. Biochemical purification characterization complexes containing fly human DEKs revealed that serve...
Squamous cell carcinoma of the cervix, highly prevalent in developing world, is often metastatic and treatment resistant with no standard protocol. Our laboratory pioneered patient-derived orthotopic xenograft (PDOX) nude mouse model technique surgical implantation (SOI). Unlike subcutaneous transplant (PDX) models, PDOX models metastasize. Most importantly, metastasis pattern correlates to patient. In present report, we describe development a HER-2-positive cervical cancer. Metastasis after...
Tumor stroma drives the growth and progression of cancers. A heparin-binding epidermal factor-like factor, HB-EGF, is an EGF receptor ligand that stimulates cell in autocrine or paracrine fashion. While elevated expression HB-EGF cancer cells its contribution to tumor are well documented, effects have not been clarified. Here, we show expressed stromal fibroblasts where it promotes proliferation. In uterine cervical cancers, was detected immunohistochemically proximal epithelium....
Abstract Inhibition of amyloid-β peptide (Aβ) accumulation in the brain is a promising approach for treatment Alzheimer’s disease (AD). Aβ produced by β-secretase and γ-secretase endosomes via sequential proteolysis amyloid precursor protein (APP). APP have common feature to readily cluster form multimers. Here, using multivalent library screens, we identified tetravalent peptide, LME-tet, which binds interactions. In cells, LME-tet-bound plasma membrane transported endosomes, blocking...
A recombinant clone, p M52 , containing cDNA for maize phosphoenolpyruvate carboxylase (PEPCase, EC 4. 1.1.31) was isolated from a leaf library constructed using an expression vector in Escherichia coli . The screening of the clone conveniently performed through its ability to complement phenotype (glutamate requirement) PEPCase-negative mutant E. enzyme encoded by this identical with major PEPCase maize, key C 4 -pathway, as judged allosteric properties and immunological reactivity. cloned...
A high-power single-pole double throw (SPDT) switch IC using AlGaN/GaN heterojunction field-effect transistors (HFETs) is demonstrated for the first time. The reduction of on-resistance (R/sub on/) and off-capacitance (C/sub off/) HFETs enables GaN-based that can be applied practical RF applications. novel Si-doping technique employed to reduce ohmic contact resistance, which successfully reduces R/sub on/. C/sub off/ on a sapphire substrate found smaller than SiC substrate, together with...
The tumor microenvironment is critical for metastasis to occur. Subcutaneous xenografts of tumors in immunodeficient mice are usually encapsulated and rarely metastasize as opposed orthotopic which if the original was metastatic. In present report, we were able reconstitute a metastatic by subcutaneously co-transplanting human cervical cancer cell line cancer-associated fibroblasts (CAFs), athymic mice, resulted lymph node 40% animals. contrast, no occurred from without CAFs. These results...
Design of Compression Ignition (CI) engines with improved thermal efficiencies needs better understanding the heat transfer mechanism from spray flame to combustion chamber wall. In this regard, occurring during interaction between impinging and wall, under CI engine-like conditions, is investigated in study using 3-Dimensional numerical simulations based on an Eulerian–Lagrangian framework. Simulations are performed for different fuel injection velocities (which representative pressures...
The present study was designed to investigate a possible role for ovarian steroids in the regulation of rat uterine oxytocin receptor (OTR) mRNA expression before labour. By using competitive RT-PCR system, we have previously reported that parturition associated with high levels OTR all animals examined. On other hand, near term, some rats showed while others did not. We therefore examined changes and during prostaglandin F(2)(alpha) (PGF(2)(alpha))-induced parturition; paradigm adopted...
The involvement of activin and follistatin, an activin-binding protein, in endochondral bone development was examined by sc implantation demineralized matrix rats. Immunoreactive follistatin localized proliferating chondrocytes round osteoblasts, whereas it not detected hypertrophic osteoblasts surrounding marrow. Western blot analysis also revealed that immunoreactive higher during the initial stages chondrogenesis (day 5) osteogenesis (days 11 14) lower conversion from cartilage to 9)....
myo-Inositol is an essential biomolecule that synthesized by myo-inositol monophosphatase (IMPase) from inositol monophosphate species. The enzymatic activity of IMPase inhibited lithium, a drug used for the treatment mood swings seen in bipolar disorder. Therefore, thought to have important role mechanism disorder, although details remain elusive. We screened ethyl nitrosourea mutant mouse library gene (Impa) mutations and identified Impa1 T95K missense mutation. protein possessed...
The present study created an artificial intelligence (AI)-automated diagnostics system for uterine cervical lesions and assessed the performance of these images AI diagnostic imaging pathological lesions. A total 463 colposcopic were analyzed. traditional colposcopy diagnoses compared to those obtained by image diagnosis. Next, 100 presented a panel 32 gynecologists who independently examined each in blinded fashion diagnosed them four categories tumors. Then, revisited their diagnosis after...
Oxytocin receptor (OTR) mRNA levels in the uterus dramatically increase near term human and rat. Estrogen is believed to be a potent stimulator of OTR expression. However, estrogen does not stimulate rat expression on day 18 pregnancy or progesterone-treated rats. Thus, regulation uterine responsiveness appears an important mediator action. To determine effect altering induction, ERα ER β were examined by competitive RT-PCR pregnant parturient rats, ovariectomized (OVX) virgin rats OVX In...
Ligand-bound nuclear receptors (NR) activate transcription of the target genes. This activation is coupled with histone modifications and chromatin remodeling through function various coregulators. However, nature dependence a NR coregulator action on presence environment at genes unclear. To address this issue, we have developed modified position effect variegation experimental model system that includes an androgen-dependent reporter transgene inserted into either pericentric...
HB-EGF is a member of the EGF family growth factors that bind and activate receptor. synthesized as membrane-anchored protein (proHB-EGF), then proteolytically cleaved, resulting in mitogenically active soluble form. ProHB-EGF functions receptor for diphtheria toxin (DT). plays pivotal roles pathophysiological processes, including cancer. Monoclonal antibodies (mAbs) specific could be an important tool research. However, few such mAbs have been established to date. In this study, we newly...
Estrogen action is mediated through several types of receptors (ERs), such as ERα, ERβ and putative membrane ERs. Oxytocin receptor (OTR) ER expression levels in the rat uterus are regulated by estrogen; however, which ERs involved has not been elucidated. This study examined OTR, ERα ovariectomized rats treated with 17β-estradiol (E2), an agonist (PPT), (DPN) or estren (Es). E2 PPT increased OTR mRNA decreased 3 6 h posttreatment. DPN at h, while h. after Es treatment then declined until...
Background: Cervical cancer is a world-wide problem that requires transformative therapeutic strategies. We have previously developed patient-derived orthotopic xenograft (PDOX) nude-mouse models of this disease. In the present report, we demonstrate standard drug, cisplatinum (CDDP), highly-effective while new, highly-touted agent, nab-paclitaxel (NAB-PTX) ineffective. Materials and Methods: PDOX tumors were grown on cervix nude mice for 4 weeks after surgical implantation (SOI). Tumors...