A5113917299- Cancer Research and Treatments
- Nanoplatforms for cancer theranostics
- Virus-based gene therapy research
- Pancreatic and Hepatic Oncology Research
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Hepatocellular Carcinoma Treatment and Prognosis
- 3D Printing in Biomedical Research
- Colorectal Cancer Treatments and Studies
- Gastric Cancer Management and Outcomes
- Lung Cancer Treatments and Mutations
- Photodynamic Therapy Research Studies
- Sarcoma Diagnosis and Treatment
- Liver physiology and pathology
- Neuroendocrine Tumor Research Advances
- Cancer Genomics and Diagnostics
- Neuroblastoma Research and Treatments
- Lung Cancer Research Studies
- Liver Disease Diagnosis and Treatment
- Cancer-related Molecular Pathways
- Medical Imaging Techniques and Applications
- Pediatric Hepatobiliary Diseases and Treatments
- Organ Transplantation Techniques and Outcomes
- Metastasis and carcinoma case studies
- Gallbladder and Bile Duct Disorders
Yokohama City University
2014-2025
Kanagawa Cancer Center
2019-2024
Kanagawa Prefectural Hospital Organization
2019-2024
Advanced Cancer Therapeutics
2022
Yokohama City University Hospital
2020-2021
University of California, San Diego
2013-2020
AntiCancer (United States)
2012-2020
Teikyo University Chiba Medical Center
2015-2017
University of California System
2014-2016
American Gastroenterological Association
2015
// Yukihiko Hiroshima 1,2,3 , Yong Zhang 1 Takashi Murakami 3 Ali Maawy 2 Shinji Miwa 1,2 Mako Yamamoto Shuya Yano Sho Sato Masashi Momiyama Ryutaro Mori Ryusei Matsuyama Chishima Kuniya Tanaka Yasushi Ichikawa Michael Bouvet Itaru Endo Ming Zhao and Robert M. Hoffman AntiCancer, Inc., San Diego, CA, USA Department of Surgery, University California Yokohama City Graduate School Medicine, Yokohama, Japan Correspondence: or Zhao, email: Keywords : Pancreatic cancer, Salmonella typhimurium...
Fluorescence-guided surgery (FGS) can enable successful cancer where bright-light often cannot. There are three important issues for FGS going forward toward the clinic: (a) proper tumor labeling, (b) a simple portable imaging system operating room, and (c) patient-like mouse models in which to develop technology. The present report addresses all three.Patient colon tumors were initially established subcutaneously nonobese diabetic (NOD)/severe combined immune deficiency (SCID) mice...
A patient with high grade undifferentiated pleomorphic soft-tissue sarcoma from a striated muscle was grown orthotopically in the right biceps femoris of mice to establish patient-derived orthotopic xenograft (PDOX) model. Twenty PDOX were divided into 4 groups: G1, control without treatment; G2, Salmonella typhimurium (S. typhimurium)A1-R administered by intratumoral (i.t.) injection once week for weeks; G3, doxorubicin (DOX) intraperitoneal (i.p.) G4, S. A1-R 2 weeks followed i.p. weeks....
Squamous cell carcinoma of the cervix, highly prevalent in developing world, is often metastatic and treatment resistant with no standard protocol. Our laboratory pioneered patient-derived orthotopic xenograft (PDOX) nude mouse model technique surgical implantation (SOI). Unlike subcutaneous transplant (PDX) models, PDOX models metastasize. Most importantly, metastasis pattern correlates to patient. In present report, we describe development a HER-2-positive cervical cancer. Metastasis after...
The aim of this study is to determine the efficacy tumor-targeting Salmonella typhimurium A1-R (A1-R) on pancreatic cancer patient-derived orthotopic xenografts (PDOX). PDOX model was originally established from a patient in SCID-NOD mice. subsequently transplanted by surgical implantation (SOI) transgenic nude red fluorescent protein (RFP) mice order that stably acquired (RFP)-expressing stroma for purpose imaging tumor after passage non-transgenic visualize growth and drug efficacy. with...
Tumor-targeting Salmonella enterica serovar Typhimurium A1-R (Salmonella A1-R) had strong efficacy on a melanoma patient-derived orthotopic xenograft (PDOX) nude-mouse model. GFP-expressing highly and selectively colonized the PDOX significantly suppressed tumor growth (p = 0.021). The combination of cisplatinum (CDDP), both at low-dose, also (P 0.001). has future clinical potential for chemotherapy with CDDP melanoma, highly-recalcitrant cancer.
Quiescent cancer cells are resistant to cytotoxic agents which target only proliferating cells. Time-lapse imaging demonstrated that tumor-targeting Salmonella typhimurium A1-R (A1-R) decoyed in monolayer culture and tumor spheres cycle from G0/G1 S/G2/M, as by fluorescence ubiquitination-based cell indicator (FUCCI) imaging. infection of FUCCI-expressing subcutaneous tumors growing nude mice also quiescent cells, were the majority tumors, thereby making them sensitive agents. The...
Ewing's sarcoma is a rare and aggressive malignancy. In the present study, tumor from patient with cyclin-dependent kinase inhibitor 2A/B (CDKN2A/B) loss FUS-ERG fusion was implanted in right chest wall of nude mice to establish patient-derived orthotopic xenograft (PDOX) model. The aim study determine efficacy 4/6 (CDK4/6) insulin-like growth factor-1 receptor (IGF-1R) inhibitors on PDOX. PDOX models were randomized into following groups when volume reached 50 mm3: G1, untreated control;...
The aim of this study is to determine the efficacy neoadjuvant chemotherapy (NAC) with gemcitabine (GEM) in combination fluorescence-guided surgery (FGS) on a pancreatic cancer patient derived orthotopic xenograft (PDOX) model. A PDOX model was established from CA19-9-positive, CEA-negative tumor who had undergone pancreaticoduodenectomy for adenocarcinoma. Mice were randomized 4 groups: bright light (BLS) only; BLS+NAC; FGS and FGS+NAC. An anti-CA19-9 or anti-CEA antibody conjugated DyLight...
A patient-derived nude-mouse model of soft-tissue sarcoma has been established and treated in the following groups: (1) untreated controls; (2) gemcitabine (GEM) (80 mg/kg, ip, weekly, 3 weeks); (3) Pazopanib (100 orally, daily, weeks) (4) Salmonella typhimurium A1-R (5 × 107 CFU/body, weeks). The was resistant to GEM (p = 0.879). tended reduce tumor volume compared mice, but there no significant difference 0.115). S. significantly inhibited growth mice 0.001). only effective treatment for...
Background and Objectives Patient‐derived orthotopic xenograft (PDOX) nude‐mouse models replicate the behavior of clinical cancer, including metastasis. The objective study was to determine efficacy zoledronic acid (ZA) on metastasis a patient‐derived model pancreatic cancer. Methods In present study, we examined ZA cancer growth in PDOX model. Results monotherapy did not significantly suppress primary tumor growth. However, weight gemcitabine (GEM) combination GEM + ZA‐treated mice...
The XPA1 human pancreatic cancer cell line is dimorphic, with spindle stem-like cells and round non-stem cells. We report here the in vitro IC 50 values of for: (1) 5-fluorouracil (5-FU), (2) cisplatinum (CDDP), (3) gemcitabine (GEM), (4) tumor-targeting Salmonella typhimurium A1-R (A1-R). were significantly higher than those for 5-FU (P = 0.007) CDDP 0.012). In contrast, there was no difference between efficacy on vivo, reduced tumor weight (5-FU; P 0.028; A1-R; 0.011). only combination...
// Takashi Murakami 1,2,3 , Shukuan Li 1 Qinghong Han Yuying Tan Tasuku Kiyuna Kentaro Igarashi Kei Kawaguchi Ho Kyoung Hwang Miyake Arun S. Singh 4 Scott D. Nelson 5 Sarah M. Dry Yunfeng Yukihiko Hiroshima 3 Thinzar Lwin 2 Jonathan C. DeLong Chishima Kuniya Tanaka Michael Bouvet Itaru Endo Fritz Eilber 6 and Robert Hoffman 1,2 AntiCancer, Inc., San Diego, California, USA Department of Surgery, University Gastroenterological Graduate School Medicine, Yokohama City University, Yokohama, Japan...
The phase of the cell cycle can determine whether a cancer respond to given drug. We report here on results monitoring real-time dynamics cells throughout live tumor intravitally using fluorescence ubiquitination indicator (FUCCI) before, during, and after chemotherapy. In nascent tumors in nude mice, approximately 30% center are G0/G1 70% S/G2/M. contrast, 90% 80% total an established phase. Similarly, 75% far from (>100 µm) blood vessels G0/G1. Longitudinal imaging demonstrated that...
Invasive cancer cells are a critical target in order to prevent metastasis. In the present report, we demonstrate real-time visualization of cell cycle kinetics invading 3-dimensional (3D) Gelfoam® histoculture, which is vivo-like. A fluorescence ubiquitination indicator (FUCCI) whereby G0/G1 express red fluorescent protein and S/G2/M green was used determine position non-invading cells. With FUCCI 3D confocal imaging, observed that phase histoculture migrated more rapidly further than...
Photoimmunotherapy (PIT) of cancer utilizes tumor-specific monoclonal antibodies conjugated to a photosensitizer phthalocyanine dye IR700 which becomes cytotoxic upon irradiation with near infrared light. In this study, we aimed evaluate the efficacy PIT on human pancreatic cells in vitro and vivo an orthotopic nude mouse model. The binding capacity anti-CEA antibody BxPC-3 was determined by FACS analysis. An cytotoxicity assay used determine cell death following treatment PIT. For...
// Yasunori Matsumoto 1,2,3,* , Shinji Miwa 1,2,4,* Yong Zhang 1 Ming Zhao Shuya Yano 1,2 Fuminari Uehara Mako Yamamoto Yukihiko Hiroshima Makoto Toneri Michael Bouvet 2 Hisahiro Matsubara 3 Hiroyuki Tsuchiya 4 and Robert M. Hoffman AntiCancer, Inc, San Diego, CA, USA Department of Surgery, University California Frontier Graduate School Medicine, Chiba University, Chiba, Japan Orthopaedic Medical Sciences, Kanazawa Ishikawa, * These authors contributed equally to this work Correspondence:...
Patient-derived xenograft (PDX) mouse models of cancer are emerging as an important component personalized precision therapy. However, most currently offered to patients have their tumors subcutaneously-transplanted in immunodeficient mice, which rarely metastasize. In contrast, orthotopic-transplant patient-derived models, termed orthotopic xenografts (PDOX), usually metastasize the patient. We demonstrate present report why so for patient, since primary and metastatic developed model can...
// Takashi Murakami 1, 2, 3 , Kentaro Igarashi 1 Kei Kawaguchi Tasuku Kiyuna Yong Zhang Ming Zhao Yukihiko Hiroshima Scott D. Nelson 5 Sarah M. Dry Yunfeng Li Jane Yanagawa 6 Tara Russell Noah Federman 7 Arun Singh 4 Irmina Elliott Ryusei Matsuyama Chishima Kuniya Tanaka Itaru Endo Fritz C. Eilber Robert Hoffman 2 AntiCancer, Inc., San Diego, California, USA Department of Surgery, University Gastroenterological Graduate School Medicine, Yokohama City University, Yokohama, Japan Division...
We have previously developed mouse models of HER-2-positive cervical cancer. Tumors in nude mice had histological structures similar to the original tumor and were stained by anti-HER-2 antibody same pattern as patient's also tumor-targeting Salmonella typhimurium A1-R demonstrated its efficacy against patient-derived models, both alone combination. In current study, we determined S. combination with trastuzumab on a patient-cancer nude-mouse model HER-2 positive Mice randomized 5 groups...
We report here the efficacy of tumor-targeting Salmonella typhimurium A1-R (A1-R) on mouse models disseminated and metastatic ovarian cancer. The proliferation-inhibitory human cancer cell lines (SKOV-3-GFP, OVCAR-3-RFP) was initially demonstrated in vitro. Orthotopic dissemination were made with line SKOV-3-GFP. After tumor implantation, mice treated (5 × 107 colony-forming units [CFU], i.v.), there no severe adverse events observed. In orthotopic model, volume after treatment 276 ± 60.8...