A5015421086- Cancer Research and Treatments
- Virus-based gene therapy research
- Animal Nutrition and Physiology
- Nanoplatforms for cancer theranostics
- Cancer, Hypoxia, and Metabolism
- RNA Interference and Gene Delivery
- Cancer Cells and Metastasis
- Photodynamic Therapy Research Studies
- Physiological and biochemical adaptations
- CAR-T cell therapy research
- Meat and Animal Product Quality
- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- Orthopaedic implants and arthroplasty
- Bird parasitology and diseases
- Animal Behavior and Welfare Studies
- Heart Rate Variability and Autonomic Control
- Hip and Femur Fractures
- Animal Genetics and Reproduction
- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Peptidase Inhibition and Analysis
- Cancer-related Molecular Pathways
- CRISPR and Genetic Engineering
- Avian ecology and behavior
Okayama University
2016-2025
University of North Texas
2012-2025
Okayama University Hospital
2016-2025
Okayama Shoka University
2012-2025
Akita Red Cross Hospital
2014-2024
Akita University
1999-2023
Akita Industrial Technology Center
2016-2020
Yamagata University
1976-2015
Graduate School USA
2015
Innovative Clinical Research
2014
Accumulating evidence suggests a role for microRNAs in human carcinogenesis as novel types of tumor suppressors or oncogenes. However, their precise biological remains largely elusive. In the present study, we aimed to identify microRNA species involved regulation cell proliferation. Using quantitative RT-PCR analysis, demonstrated that miR-34a was highly up-regulated colon cancer line, HCT 116, treated with DNA-damaging agent, adriamycin. Transient introduction into two lines, 116 and RKO,...
Purpose: Cancer-associated fibroblasts (CAFs) in the tumor microenvironment (TME) play a central role progression. We investigated whether CAFs can regulate tumor-infiltrating lymphocytes (TILs) and their immunosuppression.Experimental Design: A total of 140 cases esophageal cancer were analyzed for CD8+ or forkhead box protein 3 (FoxP3+) TILs by IHC. cytokines using murine human cells. Murine-derived cells also inoculated into BALB/c BALB/c-nu/nu mice tumors treated with recombinant IL6...
Colony-stimulating factor 1 (CSF1) is a primary regulator of the survival, proliferation, and differentiation monocyte/macrophage that sustains protumorigenic functions tumor-associated macrophages (TAMs). Considering current advances in understanding role inflammatory tumor microenvironment, targeting components sarcoma such as TAMs, viable strategy. Here, we investigated effect PLX3397 (pexidartinib) potent inhibitor CSF1 receptor (CSF1R). was recently approved by Food Drug Administration...
Quiescent cancer cells are resistant to cytotoxic agents which target only proliferating cells. Time-lapse imaging demonstrated that tumor-targeting Salmonella typhimurium A1-R (A1-R) decoyed in monolayer culture and tumor spheres cycle from G0/G1 S/G2/M, as by fluorescence ubiquitination-based cell indicator (FUCCI) imaging. infection of FUCCI-expressing subcutaneous tumors growing nude mice also quiescent cells, were the majority tumors, thereby making them sensitive agents. The...
The peritoneum is one of the most frequent metastatic sites in pancreatic cancer patients, and peritoneal dissemination makes this disease refractory due to aggressive progression chemoresistance. Although role tumor microenvironment development recognized, correlation between environment refractoriness remains unclear. intraperitoneal tumor-microenvironment its potential chemo-refractoriness, focusing especially on macrophages, were investigated.Peritoneal washes obtained from cellular...
Lymph node metastasis is a pathognomonic feature of spreading tumors, and overcoming challenge in attaining more favorable clinical outcomes. Esophageal cancer an aggressive tumor for which lymph strong poor prognostic factor, the microenvironment (TME), cancer-associated fibroblasts (CAFs) particular, has been implicated esophageal progression. CAFs play central role TME have reported to provide suitable conditions progression cancer, similar their other malignancies. However, little known...
Cancer-associated fibroblasts (CAFs) have an important role in the tumor microenvironment. CAFs multifunctionality which strongly support cancer progression and acquisition of therapeutic resistance by cells. Near-infrared photoimmunotherapy (NIR-PIT) is a novel treatment that uses highly selective monoclonal antibody (mAb)-photosensitizer conjugate. We developed fibroblast activation protein (FAP)-targeted NIR-PIT, IR700 was conjugated to FAP-specific target (CAFs-targeted NIR-PIT:...
Abstract Background Cancer-associated fibroblasts (CAFs) in the tumor microenvironment (TME) play a critical role immunosuppression. However, targeted depletion of CAFs is difficult due to their diverse cells origin and resulting lack specific surface markers. Near-infrared photoimmunotherapy (NIR-PIT) novel cancer treatment that leads rapid cell membrane damage. Methods In this study, we used anti-mouse fibroblast activation protein (FAP) antibody target FAP + (FAP-targeted NIR-PIT)...
Abstract Background The mechanisms by which neutrophils acquire pro-tumor properties remain poorly understood. In pancreatic cancer, cancer-associated fibroblasts (CAFs) may interact with neutrophils, directing them to promote tumor progression. Methods To validate the association between CAFs and localization of was examined in clinically resected cancer specimens. were produced culturing cancer-conditioned media, effects these on examined. vitro migration invasion assays assess effect...
There is evidence for incipient thermogenesis in the embryos of precocial species birds during later stages incubation. In ovo, nascent thermoregulation limited by (1) high thermal conductance egg and embryonic tissues, (2) rate at which oxygen can diffuse into through shell chorioallantoic membrane ("oxygen-conductance limited"), (3) degree development thyroid gland metabolic capacity embryo ("power limited"). with a semiprecocial mode development, hatching appears to be climactic event...
The presence of circulating tumor cells (CTCs) in the peripheral blood is associated with short survival, making detection CTCs clinically useful as a prognostic factor disease outcome and/or surrogate marker treatment response. Recent technical advances immunocytometric analysis and quantitative real-time PCR have made it possible to detect few blood; however, there no sensitive assay specifically viable CTCs. Here, we report what believe be new approach visually live human among millions...
The avian embryo exchanges the oxygen and carbon dioxide with ambient air by diffusion. respiratory organ is chorioallantois, endowed a rich circulation. Between chorioallantoic capillary blood are interposed porous shell fibrous membranes, chorioendothelium which compose diffusion barrier. cell formed between two membranes in blunt end of egg. barrier divided into an outer (shell plus membrane) inner (inner membrane blood). resistance to gas (the reciprocal diffusive conductance) almost...
The phase of the cell cycle can determine whether a cancer respond to given drug. We report here on results monitoring real-time dynamics cells throughout live tumor intravitally using fluorescence ubiquitination indicator (FUCCI) before, during, and after chemotherapy. In nascent tumors in nude mice, approximately 30% center are G0/G1 70% S/G2/M. contrast, 90% 80% total an established phase. Similarly, 75% far from (>100 µm) blood vessels G0/G1. Longitudinal imaging demonstrated that...
Invasive cancer cells are a critical target in order to prevent metastasis. In the present report, we demonstrate real-time visualization of cell cycle kinetics invading 3-dimensional (3D) Gelfoam® histoculture, which is vivo-like. A fluorescence ubiquitination indicator (FUCCI) whereby G0/G1 express red fluorescent protein and S/G2/M green was used determine position non-invading cells. With FUCCI 3D confocal imaging, observed that phase histoculture migrated more rapidly further than...
Abstract Purpose: Because chemoradiotherapy selectively targets proliferating cancer cells, quiescent stem–like cells are resistant. Mobilization of the cell cycle in leukemia stem sensitizes them to death signals. However, it is unclear that mobilization can eliminate solid cancers. Thus, we explored use a genetically-engineered telomerase-specific oncolytic adenovirus, OBP-301, mobilize and kill cells. Experimental Design: We established CD133+ from human gastric MKN45 MKN7 investigated...
Cancer-associated fibroblasts (CAFs) are strongly implicated in tumor progression, including the processes of tumorigenesis, invasion, and metastasis. The targeting CAFs using various therapeutic approaches is a novel treatment strategy; however, efficacy such therapies remains limited. Recently, near-infrared photoimmunotherapy (NIR-PIT), which targeted therapy employing cell-specific mAb conjugated to photosensitizer, has been introduced as new type phototherapy. In this study, we have...
The clinical benefit of monotherapy involving immune checkpoint inhibitors (ICIs) such as anti-programmed death-1 antibody (PD-1 Ab) is limited to small populations. We previously developed a telomerase-specific oncolytic adenovirus, Telomelysin (OBP-301), the safety which was confirmed in phase I study. Here, we examined potential OBP-502, an OBP-301 variant, agent for inducing immunogenic cell death (ICD) and synergistically enhancing efficacy OBP-502 with PD-1 Ab using CT26 murine colon...