A5001757088- Neuroscience and Neuropharmacology Research
- Alzheimer's disease research and treatments
- Neural dynamics and brain function
- Memory and Neural Mechanisms
- Neuroscience and Neural Engineering
- Neuroendocrine regulation and behavior
- Computational Drug Discovery Methods
- Receptor Mechanisms and Signaling
- Cholinesterase and Neurodegenerative Diseases
- Stress Responses and Cortisol
- Epilepsy research and treatment
- Nicotinic Acetylcholine Receptors Study
- Analytical Chemistry and Chromatography
- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Cell Adhesion Molecules Research
- Nuclear Receptors and Signaling
- Hearing, Cochlea, Tinnitus, Genetics
- Advanced Memory and Neural Computing
- Trace Elements in Health
- Bioinformatics and Genomic Networks
- Parkinson's Disease Mechanisms and Treatments
- Heat shock proteins research
- Neuropeptides and Animal Physiology
University of Szeged
2008-2024
HUN-REN Szegedi Biológiai Kutatóközpont
2013-2015
Hungarian Academy of Sciences
2013-2015
Institute of Biochemistry
2013
Bay Zoltán Foundation for Applied Research
2009-2012
Genomics (United Kingdom)
2011
Eötvös Loránd University
2005
Hsp27 belongs to the small heat shock protein family, which are ATP-independent chaperones. The most important function of is based on its ability bind non-native proteins and inhibit aggregation incorrectly folded maintaining them in a refolding-competent state. Additionally, it has anti-apoptotic antioxidant activities. To study effect memory synaptic functions, amyloid-β (Aβ) accumulation, neurodegeneration, we generated transgenic mice overexpressing human crossed with APPswe/PS1dE9...
In the human neocortex, single excitatory pyramidal cells can elicit very large glutamatergic EPSPs (VLEs) in inhibitory GABAergic interneurons capable of triggering their firing with short (3–5 ms) delay. Similar strong connections between two individual neurons have not been found nonhuman cortices, suggesting that these synapses are specific to interneurons. The VLEs crucial for generating neocortical complex events, observed as cell spike-evoked discharge assemblies frontal and temporal...
During the past 15 years, several genetically altered mouse models of human Alzheimer's disease (AD) have been developed. These costly greatly facilitated evaluation novel therapeutic approaches. Injecting synthetic β-amyloid (Aβ) 1-42 species into different parts brain non-transgenic rodents frequently provided unreliable results, owing to a lack genuine characterization administered Aβ aggregates. Previously, we published new rat AD-model in which protofibrillar-fibrillar Aβ1-42 was...
Accumulating evidence indicates that there are substantial species differences in the properties of mammalian neurons, yet theories on circuit activity and information processing human brain based heavily results obtained from rodents other experimental animals. This knowledge gap may be particularly important for understanding neocortex, area responsible most complex neuronal operations showing greatest evolutionary divergence. Here, we examined electrophysiological mouse fast-spiking...
Alzheimer's disease (AD) is the most prevalent form of neurodegenerative disorders characterized by neuritic plaques containing amyloid-β peptide (Aβ) and neurofibrillary tangles. Evidence has been reported that Aβ1-42 plays an essential pathogenic r
Inhibitory autapses are self-innervating synaptic connections in GABAergic interneurons the brain. Autapses neocortical layers have not been systematically investigated, and their function different mammalian species specific interneuron types is poorly known. We investigated parvalbumin-expressing basket cells (pvBCs) layer 2/3 (L2/3) human tissue resected deep-brain surgery, mice as control. Most pvBCs showed robust GABAAR-mediated self-innervation both species, but were rare...
Background and Aims Unnatural self-organizing biomimetic polymers (foldamers) emerged as promising materials for biomolecule recognition inhibition. Our goal was to construct multivalent foldamer-dendrimer conjugates which wrap the synaptotoxic β-amyloid (Aβ) oligomers with high affinity through their helical foldamer tentacles. Oligomeric Aβ species play pivotal role in Alzheimer's disease, therefore direct inhibition of this undruggable target is a great current challenge. Methods Results...
Abstract In the human neocortex, solitary action potentials in some layer 2–3 pyramidal cells (PCs) trigger brief episodes of network activity known as complex events through strong excitatory synapses that specifically innervate GABAergic interneurons. Yet, how these “master PCs” configure local is not well understood. We report single spikes PCs, studied here synaptically connected cell pairs frontal or temporal neocortical areas both males and females, elicit firing fast-spiking basket...
Aging is associated with the slowdown of neuronal processing and cognitive performance in brain; however, exact cellular mechanisms behind this deterioration humans are poorly elucidated. Recordings human acute brain slices prepared from tissue resected during surgery enable investigation changes age. Although neocortical fast-spiking cells widely implicated network activities underlying processes, they vulnerable to neurodegeneration. Herein, we analyzed electrical properties 147...
The effects of Alzheimer's disease-related beta-amyloid (Abeta) peptides on the N-methyl-D-aspartate (NMDA)-evoked cell firing rate were studied in hippocampal CA1 neurons rat. Extracellular single-unit recordings combined with iontophoretic applications that allowed quantitative analyses interactions between Abeta and NMDA receptor-mediated events vivo. responses significantly increased both by full length Abeta1-42 its model fragment Abeta25-35. Enhancements lasted about 15 min...
Oligomeric amyloid-β is currently of interest in mediated toxicity and the pathogenesis Alzheimer’s disease. Mapping interaction partners could help to discover novel pathways disease pathogenesis. To partners, we applied a protein array with more than 8100 unique recombinantly expressed human proteins. We identified 324 proteins as potential interactors oligomeric amyloid-β. The Gene Ontology functional analysis these showed that bound multiple diverse functions both from extra...
Aims ApoB-100 is the major protein component of cholesterol- and triglyceride-rich LDL VLDL lipoproteins in serum. Previously, we generated partially described transgenic mice overexpressing human protein. Here, further characterize this strain order to reveal a possible link between hypeprlipidemia neurodegeneration. Methods Results We analyzed serum cerebral lipid profiles, tau phosphorylation patterns, amyloid plaque-formation, neuronal apoptosis synaptic plasticity young (3 month old),...
Neuronal hyperexcitability is a phenomenon associated with early Alzheimer's disease. The underlying mechanism considered to involve excessive activation of glutamate receptors; however, the exact molecular pathway remains be determined. Extracellular recording from CA1 hippocampal slices long-standing standard for range studies both in basic research and neuropharmacology. Evoked field potentials (fEPSPs) are regarded as input, while spiking rate output neuronal network; relationship...
The underlying cause of Alzheimer's disease (AD) is thought to be the beta-amyloid aggregates formed mainly by Abeta1-42 peptide. Protective pentapeptides [e.g., Leu-Pro-Phe-Phe-Asp (LPFFD)] have been shown prevent neuronal toxicity arresting and reversing fibril formation. Here we report that an endogenous tetrapeptide, endomorphin-2 (End-2, amino acid sequence: YPFF), defends against induced neuromodulatory effects at cellular level. Although End-2 does not interfere with kinetics Abeta...
The aggregated form of amyloid-beta (Abeta) (1-42) has been shown to increase N-methyl-D-aspartic acid (NMDA) evoked neuronal activity in vivo. Here we further characterized this phenomenon by investigating the role integrin activation and downstream Src kinase using vivo electrophysiology vitro intracellular Ca (2+) measurements. Pretreatment differentiated SH-SY5Y cells with fibrillar Abeta markedly enhanced calcium increases caused NMDA receptor (NMDA-R) stimulation. Function blocking...
The underlying cause of Alzheimer's disease (AD) is thought to be the accumulation and aggregation a misfolded protein, amyloid-β (Aβ). A promising strategy against AD application protective, peptide-based neuroprotective agents that sel