A5004007941- Tryptophan and brain disorders
- Advanced Drug Delivery Systems
- Neuropeptides and Animal Physiology
- Drug Solubulity and Delivery Systems
- Metabolomics and Mass Spectrometry Studies
- Advancements in Transdermal Drug Delivery
- Migraine and Headache Studies
- Neuroendocrine regulation and behavior
- Pharmacological Receptor Mechanisms and Effects
- Receptor Mechanisms and Signaling
- Analytical Chemistry and Chromatography
- Analytical Methods in Pharmaceuticals
- Ginseng Biological Effects and Applications
- Mass Spectrometry Techniques and Applications
- Urinary Tract Infections Management
- Lipid Membrane Structure and Behavior
- Mental Health Research Topics
- Gut microbiota and health
- Neuroinflammation and Neurodegeneration Mechanisms
- Inflammatory mediators and NSAID effects
- Chemical Synthesis and Analysis
- Pain Mechanisms and Treatments
- Multiple Sclerosis Research Studies
- Neuroscience and Neuropharmacology Research
- Reproductive tract infections research
University of Szeged
2017-2023
Abstract Background Altered glutamatergic neurotransmission and neuropeptide levels play a central role in migraine pathomechanism. Previously, we confirmed that kynurenic acid, an endogenous antagonist, was able to decrease the expression of pituitary adenylate cyclase-activating polypeptide 1–38, with known migraine-inducing properties. Hence, our aim reveal peripheral kynurenine pathway (KP) episodic migraineurs. We focused on complete tryptophan (Trp) catabolism, which comprises...
The aim of this study was to optimize the formulation meloxicam (MEL)-containing human serum albumin (HSA) nanoparticles for nose-to-brain via a quality by design (QbD) approach. Liquid and dried formulations containing Tween 80 without surfactant were investigated. Various properties, such as Z-average, zeta potential, encapsulation efficacy (EE), conjugation MEL HSA, physical stability, in vitro dissolution, permeability, vivo plasma brain distribution characterized. From stability point...
The glutamatergic neurotransmission has important role in the pathomechanism of primary headache disorders. kynurenine metabolites derived from catabolism tryptophan (Trp) have significant involvement not only processes, but also neuroinflammation, oxidative stress and mitochondrial dysfunctions. Previously we identified a depressed peripheral Trp metabolism interictal period episodic migraineurs, which prompted us to examine this pathway patients with cluster (CH) as well. Our aims were...
The aim of this article was to study the trans-epithelial absorption reach blood and target brain by axonal transport using nasal formulations with nanonized meloxicam (nano MEL spray) its salt form known as potassium monohydrate (MELP spray). physicochemical properties mucoadhesivity were controlled. In vitro in vivo studies carried out. These forms first investigated “nose-to-brain” relation. It found that did not show any significant correlation. experiments demonstrated faster...
The aim of this work was to study the influence solidification meloxicam (Mel) containing nanosuspension (nanoMel) on physical stability and drug bioavailability products. nanoMel sample had poly(vinyl alcohol) (PVA) as a protective polymer, but no surfactant further stabilizing agent because final produce surfactant-free solid phase products well.The solidified samples produced by fluidization lyophilization (fluidMel, lyoMel) were examined for particle size, crystallinity, in vitro release...
Nasal drug delivery has become a popular research field in the last years. This is not surprising since nose possesses unique anatomical and physical properties. Via nasal mucosa local, systemic, directly central nerve systemic (CNS) effect achievable. Powders have favorable physicochemical properties over liquid formulations. Lamotrigine (LAM) an antiepileptic agent with relatively mild side spectrum, but only available tablet form on market. Reducing particle size to nano range can affect...
Facile syntheses of 3-O-carbamoyl, -sulfamoyl, or -pivaloyl derivatives 13α-oestrone and its 17-deoxy counterpart have been carried out. Microwave-induced, Ni-catalysed Suzuki-Miyaura couplings the newly synthesised phenol esters with phenylboronic acid afforded 3-deoxy-3-phenyl-13α-oestrone derivatives. The carbamate pivalate proved to be suitable for regioselective arylations. 2-(4-Substituted) phenyl were via Pd-catalysed, microwave-assisted C-H activation reactions. An efficient,...
Neutrophil granulocytes are the major cells involved in Chlamydia trachomatis (C. trachomatis)-mediated inflammation and histopathology. A key protein human intracellular antichlamydial defense is tryptophan-degrading enzyme indoleamine 2,3-dioxygenase (IDO) which limits growth of tryptophan auxotroph Chlamydia. Despite its importance, role IDO against neutrophils not well characterized.Global gene expression screen was used to evaluate effect C. serovar D infection on transcriptome...
Biomarkers for predicting treatment response to thrombolysis in acute ischemic stroke are currently lacking. Both, animal models and clinical studies have provided evidence that the kynurenine (KYN) pathway is activated stroke.In our pilot study, we aimed investigate whether KYN enzymes metabolites could serve as potential biomarkers hyperacute phase of stroke.We included 48 patients who received thrombolysis. Blood samples were taken both before 12 h after treatment. Concentrations 11...
Abstract Glycerophospholipids (PLs), as amphipathic small molecules and the main constituents of biological membranes, play an important role in several cellular processes, even though their accurate identification from complex samples remains a challenge. In this paper, we report fast comprehensive HILIC‐ESI‐MS method for analysis glycerophospholipid classes using high‐resolution mass spectrometry negative mode. The final enabled quantitative 130 endogenous PL species mouse plasma....
Platelets regulate cell-cell interactions and local circulation through eicosanoids from arachidonic acid. Sigma non-opioid intracellular receptor 1 (sigma-1 receptor) expressed in platelets endothelial cells can signalization. Our aim was to examine the influence of sub-chronic, vivo-administered sigma-1 ligands 2-morpholin-4-ylethyl 1-phenylcyclohexane-1-carboxylate (PRE-084); N-benzyl-2-[(1S)-6,7-dimethoxy-1,2,3,4-tetrahydroisoquinolin-1-yl]ethan-1-amine; dihydrochloride, a new compound...
<title>Abstract</title> <bold>Background:</bold>The glutamatergic neurotransmission has important role in the pathomechanism of primary headache disorders. The kynurenine metabolites derived from catabolism tryptophan (Trp) have significant involvement not only processes, but also neuroinflammation, oxidative stress and mitochondrial dysfunctions. Previously we identified a depressedperipheral Trp metabolism interictal period episodic migraineurs, which prompted us to examine this pathway...
Abstract Diabetes mellitus is a chronic metabolic disorder which induces endothelial dysfunction and platelet activation. Eicosanoids produced from arachidonic acid regulate cellular vascular functions. Sigma-1 receptor expressed in platelets cells can intracellular signalization. Our aim was to examine the influence of sub-chronic, vivo administered sigma-1 ligands (2-(4-morpholino)ethyl-1-phenylcyclohexane-1-carboxylate, PRE-084;...
Background Diabetes mellitus is a chronic metabolic disorder which induces endothelial dysfunction and platelet activation. Eicosanoids produced from arachidonic acid regulate cellular vascular functions. Sigma-1 receptors (S1R) are expressed in platelets cells S1R expression protective diabetes. Objectives Our aim was to examine the influence of sub-chronic, vivo administered ligands PRE-084, (S) -L1 (a new compound) NE-100 on ex metabolism aorta streptozotocin-induced diabetic rats....