A new caspase-independent mode of programmed cell death, termed necroptosis, has recently been identified. Altered expression molecules involved in the necroptosis pathway shown to trigger intestinal inflammation. The initiation is principally mediated by release receptor interacting protein 3 (RIP3) from suppression caspase-8. Furthermore, it suggested that mixed lineage kinase domain-like (MLKL) factor an target RIP3 active necroptosis. This study aims at investigating occurrence children...

Human cyclin D1 is expressed as two isoforms derived by alternate RNA splicing, termed D1a and D1b, which differ for the inclusion of intron 4 in D1b mRNA. Both are frequently upregulated human cancers, but displays relatively higher oncogenic potential. The splicing factors that regulate alternative remain unknown despite likelihood they contribute to oncogenicity. In this study, we report Sam68, an RNA-binding protein overexpressed prostate cancer cells, enhances supports its expression...

High-mobility group box 1 (HMGB1) is a nuclear protein with functions in the regulation of transcription. In inflammatory conditions, HMGB1 actively secreted from immune cells extracellular matrix, where it behaves as proinflammatory cytokine. The aim present study was to investigate role pediatric bowel disease (IBD).We analyzed stools 19 children Crohn's (CD), 21 ulcerative colitis (UC), and 13 controls. gene/protein expression levels were assessed bioptic specimens all using real-time PCR...

Crohn's disease (CD) and ulcerative colitis (UC), known as inflammatory bowel diseases (IBD), are characterized by an abnormal immunological response to commensal bacteria colonizing intestinal lumen mucosa. Among the latter, strains of adherent-invasive Escherichia coli (AIEC), capable adhering invading epithelium, replicate in macrophages, have been described CD adults. We aimed at identifying characterizing AIEC pediatric IBD.In all, 24 children, 10 UC, 23 controls were investigated....

Large evidence supports the role of microRNAs as new important inflammatory mediators by regulating both adaptive and innate immunity. In present study, we speculated that miR-320 controls NOD2 (nucleotide-binding oligomerization domain) expression, because it contains multiple binding sites in 3′-untranslated region gene. NOD2, first gene associated to increased susceptibility Crohn's disease, is a cytosolic receptor senses wall peptides bacteria promotes their clearance through initiation...

Noninvasive biomarkers of high- and low-grade intestinal inflammation mucosal healing (MH) in patients with inflammatory bowel disease are currently lacking. We have recently shown that fecal high mobility group box 1 (HMGB1) protein is a novel biomarker gut inflammation. aimed at investigating mouse model if HMGB1 was able to foresee both clinically evident subclinical its normalization indicated MH. also confirming the results Crohn's (CD) ulcerative colitis.C57BL6/J mice were treated...

Chronic inflammatory bowel disorders (IBD) are idiopathic diseases associated with altered intestinal permeability, which in turn causes an exaggerated immune response to enteric antigens a genetically susceptible host. A rise psych cognitive disorders, such as anxiety and depression, has been observed IBD patients. We here report investigations on model of chemically induced experimental colitis by oral administration sodium dextran sulfate (DSS) C57BL/6 mice. investigate, vivo, the...

Faecal biomarkers have emerged as important tools in managing of inflammatory bowel disease [IBD], which includes Crohn's [CD] and ulcerative colitis [UC].To identify new gut inflammation the stools IBD patients using a proteomic approach.Proteomic analysis was performed with both active CD remission controls by 2-DIGE MALDI-TOF/TOF MS. An ELISA used to confirm results second cohort controls.2-DIGE detected 70 spots or controls. MS identified 21 proteins Chymotrypsin C, Gelsolin Rho...

Electrospinning is an advanced manufacturing strategy used to create innovative medical devices from continuous nanoscale fibers that endowed with tunable biological, chemical, and physical properties. Innovative patches manufactured entirely by electrospinning are discussed in this paper, using a specific plant-derived formulation “1 Primary Wound Dressing©” (1-PWD) as active pharmaceutical ingredient (API). 1-PWD composed of neem oil (Azadirachta indica A. Juss.) the oily extracts...

This study reports on the two-step manufacturing process of a filtration media obtained by first electrospinning layer polycaprolactone (PCL) non-woven fibers onto paper filter backing and subsequently coating it electrospraying with second made pure acidolysis lignin. The lignin coatings solution represents novel development that requires fine control underlying electrodynamic processing. effect increasing deposition time was investigated for electrospray from 2.5 min to 120 min....

Abstract Background and aims Intestinal fibrosis is a common complication of Inflammatory Bowel Disease (IBD), namely Crohn's disease (CD) ulcerative colitis (UC), but the precise mechanism by which it occurs incompletely understood hampering development effective therapeutic strategies. Here, we aimed at inducing characterizing an inflammation-mediated in patient-derived organoids (PDOs) issued from crypts isolated colonic mucosal biopsies IBD pediatric patients age matched-control subjects...

Abstract Coexpression for c‐Kit receptor and its ligand stem cell factor (SCF) has been described in neuroblastoma (NB) lines tumors, suggesting the existence of an autocrine loop modulating tumor growth. We evaluated SCF expression by immunohistochemistry a series 75 primary newly diagnosed neuroblastic tumors. Immunostaining was found 10/75 17/75, with 5/10 c‐Kit–positive tumors also expressing SCF. For both, staining were predominantly most aggressive subset i.e ., those amplified MYCN :...

Signaling through insulin-like growth factor I receptor (IGF-IR) is important for and survival of many tumor types. Neuroblastoma sensitive to IGF.We assessed the ability NVP-AEW541, a recently developed small molecule that selectively inhibits IGF-IR activity, neuroblastoma effects in vitro vivo. Our data showed that, panel 10 cell lines positive expression, NVP-AEW541 inhibited proliferation submicromolar/micromolar (0.4-6.8) range concentrations.As expected, IGF-II-mediated stimulation...

Abstract Purpose: We assessed the relevance of Slug (SNAI2) for apoptosis resistance and invasion potential neuroblastoma cells in vitro vivo. Experimental Design: evaluated effect imatinib mesylate on analyzed genes modulated by treatment cells. expression, inhibited treatment, was knocked down RNA interference, effects were vitro. A pseudometastatic model severe combined immunodeficient mice used to assess silencing alone or combination with metastasis development. Results: Microarray...

Background High mobility group box-1 (HMGB1) is a DNA-binding protein that released from injured cells during inflammation. Advances in targeting HMGB1 represent major challenge to improve the treatment of acute/chronic Aim This study aimed at verifying whether inhibition through dipotassium glycyrrhizate (DPG) good strategy reduce intestinal Methods Human colon adenocarcinoma cell line, HT29, human epithelial colorectal adenocarcinoma, Caco2, and murine macrophage RAW 264.7, were cultured...

Fecal high mobility group box 1 (HMGB1) has been suggested to be a novel noninvasive biomarker of gut inflammation. We aimed assess the reliability fecal HMGB1, compared with calprotectin (FC), in detecting intestinal inflammation pediatric and adult patients inflammatory bowel disease (IBD) evaluate accuracy HMGB1 identifying IBD clinical endoscopic remission who still have histologic features inflammation.Stool samples from 85 children (49 Crohn's [CD] 36 ulcerative colitis [UC] 119 adults...

Porous scaffolds made of biocompatible and environmental-friendly polymer fibers with diameters in the nano/micro range can find applications a wide variety sectors, spanning from biomedical field to textiles so on. Their development has received boost last decades thanks advances production methods, such as electrospinning technique. Conferring antimicrobial properties these fibrous structures is primary requirement for many their applications, but addition agents by wet methods present...

Extracellular High-mobility group box 1 (HMGB1) contributes to the pathogenesis of inflammatory disorders, including bowel diseases (IBD). Poly (ADP-ribose) polymerase (PARP1) has been recently reported promote HMGB1 acetylation and its secretion outside cells. In this study, relationship between PARP1 in controlling intestinal inflammation was explored. C57BL6/J wild type (WT) PARP1-/- mice were treated with DSS induce acute colitis, or inhibitor, PJ34. Human organoids, which are originated...

Current research into original therapies to treat intestinal inflammation is focusing on no-drug therapies. KLD a mixture of krill oil (KO), probiotic Lactobacillus reuteri (LR), and vitamin D (VitD3). The aim this study was assess in vitro vivo the potential cooperative effects reducing gut inflammation. Colorectal adenocarcinoma cell lines, CACO2 HT29, C57BL/6 mice were used for analyses, respectively. Cells exposed cytomix (interferon gamma + tumour necrosis factor alpha (TNF-α)) induce...

Gut mucosal healing (MH) is considered a key therapeutic target and prognostic parameter in the management of inflammatory bowel disease (IBD). The dipotassium glycyrrhizate (DPG), salt glycoconjugated triterpene glycyrrhizin, has been shown to inhibit High Mobility Group Box 1 (HMGB1) protein, an allarmin strongly implicated pathogenesis most auto-immune disorders. Here we discuss new insights on how DPG acts MH comparing acute phase recovery from experimental colitis mice. We found that...