A5025839843- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Burn Injury Management and Outcomes
- Amino Acid Enzymes and Metabolism
- Metabolism and Genetic Disorders
- Aluminum toxicity and tolerance in plants and animals
- Thermoregulation and physiological responses
- Mesenchymal stem cell research
- Wound Healing and Treatments
- Enzyme Structure and Function
- Adipokines, Inflammation, and Metabolic Diseases
- Metabolism, Diabetes, and Cancer
- Thermal Regulation in Medicine
- Chemical Analysis and Environmental Impact
- Trace Elements in Health
- Diet and metabolism studies
- Bacterial Genetics and Biotechnology
- Cardiac Arrest and Resuscitation
- Biochemical Acid Research Studies
- Polyamine Metabolism and Applications
- Heme Oxygenase-1 and Carbon Monoxide
- Redox biology and oxidative stress
- Wastewater Treatment and Nitrogen Removal
- Metabolomics and Mass Spectrometry Studies
- Endoplasmic Reticulum Stress and Disease
Beth Israel Deaconess Medical Center
2021-2025
Howard Hughes Medical Institute
2024-2025
Harvard University
2021-2023
Hadassah Medical Center
2022
Sunnybrook Research Institute
2017-2021
Sunnybrook Hospital
2017-2021
Sunnybrook Health Science Centre
2017-2021
Health Sciences Centre
2017-2021
Canadian Institutes of Health Research
2021
Weatherford College
2021
Brown adipose tissue (BAT) is best known for thermogenesis. Rodent studies demonstrated that enhanced BAT thermogenesis tightly associated with increased energy expenditure, reduced body weight, and improved glucose homeostasis. However, human protective against type 2 diabetes, independent of weight. The mechanism underlying this dissociation remains unclear. Here, we report impaired mitochondrial catabolism branched-chain amino acids (BCAAs) in BAT, by deleting BCAA carriers (MBCs), caused...
ABSTRACT α-Ketoglutarate (KG) is a crucial metabolite in all living organisms, as it participates variety of biochemical processes. We have previously shown that this keto acid an antioxidant and plays key role the detoxification reactive oxygen species (ROS). In effort to further confirm intriguing phenomenon, Pseudomonas fluorescens was exposed menadione-containing media, with various amino acids sources nitrogen. Here, we demonstrate KG dehydrogenase (KGDH) NAD-dependent glutamate (GDH)...
One of the most significant adverse postburn responses is abnormal scar formation, such as keloids. Despite its prolificacy, underlying pathophysiology keloid development unknown. We recently demonstrated that NLRP3 inflammasome, master regulator inflammatory and metabolic (e.g., aerobic glycolysis), essential for physiological wound healing. Therefore, burn patients who develop keloids may exhibit altered immunometabolic at site injury, which interferes with normal healing portends...
Browning, the conversion of white adipose tissue (WAT) to a beige phenotype, has gained interest as strategy induce weight loss and improve insulin resistance in metabolic disorders. However, for hypermetabolic conditions stemming from burn trauma or cancer cachexia, browning is thought contribute energy wasting supraphysiological nutritional requirements. Metformin's impact on this phenomenon underlying mechanisms have not been explored. We used both murine model human ex vivo explants...
Burn patients experiencing hypermetabolism develop hepatic steatosis, which is associated with liver failure and poor outcomes after the injury. These same also undergo white adipose tissue (WAT) browning, has been implicated in mediating post-burn cachexia sustained hypermetabolism. Despite clinical presentation of steatosis WAT browning burns, whether or not these two pathological responses are linked remains poorly understood. Here, we show that burn-induced its increased lipolysis leads...
De novo beige adipocyte biogenesis involves the proliferation of progenitor cells in white adipose tissue (WAT); however, what regulates this process remains unclear. Here, we report that mouse models but also human tissues, WAT lipolysis-derived linoleic acid triggers cell following cold acclimation, β3-adrenoceptor activation, and burn injury. A subset progenitors, as marked by surface markers PDGFRα or Sca1 CD81, harbored cristae-rich mitochondria actively imported via a fatty transporter...
The role of alpha-ketoglutarate (KG) in the detoxification reactive oxygen species (ROS) has only recently begun to be appreciated. This ketoacid neutralizes ROS an NADPH-independent manner with concomitant formation succinate and CO(2). To further probe this intriguing attribute KG living systems, we have evaluated significance histidine metabolism model organism, Pseudomonas fluorescens, challenged by hydrogen peroxide (H(2)O(2)). Here, show that amino acid does contribute homeostasis...
Nitrosative stress is an ongoing challenge that most organisms have to contend with. When nitric oxide (NO) may be generated either exogenously or endogenously encounters reactive oxygen species (ROS), it produces a set of toxic moieties referred as nitrogen (RNS). As these RNS can severely damage essential biomolecules, numerous evolved elaborate detoxification strategies nullify RNS. However, the contribution cellular metabolism in fending off nitrosative poorly understood. Using variety...
Choline is an essential nutrient for the biosynthesis of phospholipids, neurotransmitters, and one-carbon metabolism with a critical step being its import into mitochondria. However, underlying mechanisms biological significance remain poorly understood. Here, we report that SLC25A48, previously uncharacterized mitochondrial inner-membrane carrier protein, controls choline transport synthesis choline-derived methyl donors. We found SLC25A48 was required brown fat thermogenesis, respiration,...
Aims To identify the molecular networks in Pseudomonas fluorescens that convey resistance to toxic concentrations of Zn, a common pollutant and hazard biological systems. Methods Results strain ATCC 13525 was cultured growth medium with millimolar Zn. Enzymatic activities metabolite levels were monitored aid in-gel activity assays high-performance liquid chromatography, respectively. As oxidative phosphorylation rendered ineffective, assimilation citric acid mediated sequentially by citrate...
Objective: The aim of this study was to uncover the mediators and mechanistic events that facilitate browning white adipose tissue (WAT) in response burns. Background: In hypermetabolic patients (eg, burns, cancer), WAT has presented substantial clinical challenges related cachexia, atherosclerosis, poor outcomes. Browning recently been found induce sustain hypermetabolism. Although appears central trauma-, burn-, or cancer-induced catabolism, are essentially unknown. Methods: blood samples...