Hydrophilic interaction chromatography (HILIC) interfaced with an Orbitrap Fourier transform mass spectrometer (FT‐MS) was used to carry out metabolomic profiling of the classical Drosophila mutation, rosy ( ry ). This gene encodes a xanthine oxidase/dehydrogenase. In addition validating technology by detecting same changes in xanthine, hypoxanthine, urate and allantoin that have been reported classically, completely unsuspected were detected each tryptophan, arginine, pyrimidine...

Curry leaves (<italic>Murraya koenigii</italic>) are a leafy spice used in Indian cookery for its fragrant aroma.

A platform based on hydrophilic interaction chromatography in combination with Fourier transform mass spectrometry was developed order to carry out metabonomics of Drosophila melanogaster strains. The method able detect approximately 230 metabolites, mainly the positive ion mode, after checking eliminate false positives caused by isotope peaks, adducts and fragment ions. Two wild-type strains, Canton S Oregon R, were studied, plus two mutant Maroon Like Chocolate. In observe differential...

Interaction of curcumin (CUR) with the enzyme dihydrofolate reductase (DHFR) was studied by molecular docking using AutoDock 4.2 as software application.AutoDock serves a valid and acceptable application to study interactions small compounds proteins.Interactions DHFR were compared those methotrexate (MTX), known inhibitor enzyme.The calculated free energy binding (∆G binding) shows that = -9.02kcal/mol; Ki 243 nM) binds affinity comparable or better than MTX -8.78kcal/mol; 363 nM).Binding...

Aim: The previously reported dual histone deacetylase type II (HDAC II) / topoisomerase I (Topo I) inhibitors suffer pharmacokinetic limitations because of their huge molecular weights. Materials & methods: We report the design and synthesis a smarter novel set uracil-linked Schiff bases (19-30) as HDAC II/Topo keeping essential pharmacophoric features. Cytotoxicity all compounds was assessed against three cancer cell lines. Studies effects on apoptotic BAX antiapoptotic BCL2 genes, docking...

Mobile phones contaminated with bacteria may act as fomites. Antibiotic resistant bacterial contamination of mobile inpatients was studied. One hundred and six samples were collected from patients admitted in various hospitals Jazan province Saudi Arabia. Eighty-nine (83.9%) out 106 found to be bacteria. Fifty-two (49.0%) coagulase-negative Staphylococcus, 12 (11.3%) Staphylococcus aureus, 7 (6.6%) Enterobacter cloacae, 3 (2.83%) Pseudomonas stutzeri, Sphingomonas paucimobilis, 2 (1.8%)...

Dengue is endemic in Saudi Arabia especially Jeddah, Makkah, Asir, and Jazan areas where pyrethroids are widely used to control the vector, Aedes aegypti. Resistance of Ae. aegypti pyrethroid insecticides has been reported from most these areas.The present study was carried out region south-west explore resistance status consequent underlying mechanisms.Three (permethrin, lambda-cyhalothrin, cyfluthrin) were investigate adults following World Health Organization (WHO) standard methods: PCR...

In silico interaction of curcumin with the enzyme MMP-3 (human stromelysin-1) was studied by molecular docking using AutoDock 4.2 as software application.AutoDock serves a valid and acceptable application to study interactions small compounds proteins.Interactions were compared those two known inhibitors enzyme, PBSA MPPT.The calculated free energy binding (∆G binding) shows that binds affinity comparable or better than inhibitors.Binding active site residues are also predicted.Curcumin...

Abstract: Girinimbine is a carbazole alkaloid isolated from the stem bark and root of Murraya koenigii. Here we report that girinimbine an inhibitor angiogenic activity both in vitro vivo. MTT results showed inhibited proliferation human umbilical vein endothelial cells, while cell invasion, migration, tube formation, wound healing assays demonstrated significant time- dose-dependent inhibition by girinimbine. A proteome profiler array done on girinimbine-treated cells had mediated...

History of the utilization various parts Camellia sinensis such as seed oil in different folklore medicaments and plants leaves for preparing green tea is very old. Pharmacokinetics (PK) plays a key role drug discovery stage by channelizing medicinal chemist optimization process chemical sequences lends hand to pharmacologists invent vivo studies. The objectives present study were elaborate quantitative determination phytochemicals nutritional assessment C. especially on composition local...

Abstract Background The expression signature of deregulated long non-coding RNAs (lncRNAs) and related genetic variants is implicated in every stage tumorigenesis, progression, recurrence. This study aimed to explore the association lncRNA cyclin-dependent kinase inhibitor 2B antisense RNA 1 ( CDKN2B-AS1 ) gene rs2383207A&gt;G intronic variant with breast cancer (BC) risk prognosis verify molecular role networks this BC by bioinformatics analysis. Methods Serum relative rs2383207 genotypes...

According to the World Health Organization, increasing antibiotic resistance of pathogens is one most important threats human health. Prevalence a carbapenem-resistance gene (KPC), vancomycin-resistance genes (van A/B) and methicillin-resistance (mecA) in hospital municipal sewages will be potential threat public health.Vancomycin-resistance were detected sewage community tank-II, tank tertiary general hospital. Carbapenem-resistance was tank-II from Methicillin-resistance fish market an...

Mahanimbine (MN) is a carbazole alkaloid present in the leaves of Murraya koenigii, which an integral part medicinal and culinary practices Asia. In study, anticancer, apoptotic anti-invasive potential MN has been delineated vitro. Apoptosis cells determination was carried out utilizing acridine orange/propidium iodide double fluorescence test. During treatment, caspase-3/7,-8, and-9 enzymes mitochondrial membrane potentials (Δψm) were evaluated. Anti-invasive properties tested wound-healing...

The conventional morphological characterization of mosquito species remains heavily used for identification in Jazan, Saudi Arabia. It requires substantial expertise and time, as well having difficulty confirming identity morphologically similar species. Therefore, to establish a reliable accurate system that can be applied understanding spatial distribution local from the Jazan region, DNA barcoding was explored an integrated tool identification. In this study, 44 adult specimens were...

Catha edulis (Khat) is an evergreen shrub or small tree, traditionally used by various peoples of the Arabian Peninsula and Africa as integral component socioeconomic traditions. It believed that psychostimulant nature toxic khat primarily due to presence cathinone cathine respectively. Studies have shown chewing closely associated with cardiac complications, especially myocardial infarction. Hence in this study, we exposed cathine-rich extract a cardiomyoblast H9c2 (2-1) cell line check...

Dengue virus (DENV) infection is a global economic and public health concern, particularly in tropical subtropical countries where it endemic. Saudi Arabia has seen an increase DENV infections, especially the western southwestern regions. This study aims to investigate genetic variants of DENV-2 that were circulating during serious outbreak Jazan region 2019.A total 482 serum samples collected 2019 from tested with reverse transcription-polymerase chain reaction (RT-PCR) detect classify...

Tuberculosis(TB) is a disease of global significance, which accounts for death in every 15 seconds. Recent studies shows TB rising certain parts the world, and Saudi Arabia one them. Several factor contribute predisposing subjects infection including but not limited to addiction various compounds have immune modulation properties, such as amphetamines Heroin etc. Khat plant whose leaves are chewed its euphoric effect east Africa Arabian Peninsula Arabia, considered mildly addictive,...

This study aimed to determine whether there is an influence of interleukin 6 ( IL-6) gene promoter polymorphisms on IL-6 plasma levels and its role in the development ischemic stroke young Indians. One hundred patients with (age ≥ 45 years) equal number age- sex-matched controls were genotyped for 174G&gt;C, −572G&gt;C, −597G&gt;A by polymerase chain reaction–restriction fragment length polymorphism. Plasma measured enzyme-linked immunosorbent assay. significantly higher as compared...

Murraya koenigii (L.) Spreng, is a significant herb of traditional Ayurvedic system medicine. Koenimbine, carbazole alkaloid isolated from this plant holds antiproliferative and apoptotic effects. The aim study was to assess koenimbine-induced DNA damage clarify the role free radicals in cell death mechanisms, using HepG2 cells.The level cytotoxicity assayed by MTT assay. To elucidate glutathione (GSH), intracellular GSH analyzed. effect koenimbine mitochondria evaluated mitochondrial...

The authors aimed to evaluate the prognostic value of miRNA-27a (miR-27a), peroxisome proliferator-activated receptor alpha/gamma (PPARα/γ) and retinoid X alpha (RXRα) tissue expression in patients with thyroid carcinoma. levels were quantified 174 archived specimens using real-time quantitative PCR. Downregulation miR-27a was associated lymph node stage multifocality. PPARα histopathological type, tumor size invasion. Moreover, RXRα lower who underwent total/subtotal thyroidectomy or...