A5082480007- Antimicrobial Resistance in Staphylococcus
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Probiotics and Fermented Foods
- Bacterial biofilms and quorum sensing
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Microbial infections and disease research
- Salmonella and Campylobacter epidemiology
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Listeria monocytogenes in Food Safety
- Streptococcal Infections and Treatments
- SARS-CoV-2 detection and testing
- Advanced biosensing and bioanalysis techniques
- Bacterial Genetics and Biotechnology
- Mycobacterium research and diagnosis
- RNA and protein synthesis mechanisms
- SARS-CoV-2 and COVID-19 Research
- Microbial Metabolism and Applications
- Enterobacteriaceae and Cronobacter Research
- Bacterial Infections and Vaccines
- Antifungal resistance and susceptibility
- Infective Endocarditis Diagnosis and Management
Royal Prince Alfred Hospital
2017-2021
The University of Sydney
2015-2021
Western Sydney University
2018
Agriculture and Agri-Food Canada
2015-2016
Abstract Viral whole-genome sequencing (WGS) provides critical insight into the transmission and evolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Long-read devices from Oxford Nanopore Technologies (ONT) promise significant improvements in turnaround time, portability cost, compared to established short-read platforms for viral WGS (e.g., Illumina). However, adoption ONT SARS-CoV-2 surveillance has been limited due common concerns around accuracy. To address this,...
Enterococcus is ubiquitous in nature and a commensal of both the bovine human gastrointestinal (GI) tract. It also associated with clinical infections humans. Subtherapeutic administration antibiotics to cattle selects for antibiotic resistant enterococci GI Antibiotic resistance genes (ARGs) may be present following use cattle. If located on mobile genetic elements (MGEs) their dissemination between species pathogenic bacteria promoted, reducing efficacy antibiotics.We comparative genomic...
Abstract Accumulating evidence supports the high prevalence of co-infections among Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) patients, and their potential to worsen clinical outcome COVID-19. However, there are few data on Southern Hemisphere populations, most studies date have investigated a narrow spectrum viruses using targeted qRT-PCR. Here we assessed respiratory viral SARS-CoV-2 patients in Australia, through virome characterization. Nasopharyngeal swabs 92...
Tylosin phosphate is a macrolide commonly administered to cattle in North America for the control of liver abscesses. This study investigated effect in-feed administration tylosin at subtherapeutic levels and its subsequent withdrawal on resistance using enterococci as an indicator bacterium. Fecal samples were collected from steers that received no antibiotics (11 ppm) 197 days withdrawn 28 before slaughter. Enterococcus species isolated fecal identified through sequencing groES-EL...
VRE are prevalent among patients in ICUs. Non-typeable vanA VRE, due to loss of one the genes used for MLST (pstS), have increased Australia, suggestive a new, hospital-acquired lineage. To understand significance this lineage and its transmission using WGS strains isolated from ICUs across New South Wales, Australia. A total 240 Enterococcus faecium isolates collected between February May 2016, identified by conventional PCR as positive, were sequenced. Isolates originated 12 grouped...
ABSTRACT Viral whole-genome sequencing (WGS) provides critical insight into the transmission and evolution of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Long-read devices from Oxford Nanopore Technologies (ONT) promise significant improvements in turnaround time, portability cost, compared to established short-read platforms for viral WGS (e.g., Illumina). However, adoption ONT SARS-CoV-2 surveillance has been limited due common concerns around accuracy. To address this,...
Whole genome sequencing (WGS) was used to demonstrate the wide genetic variability within Sporothrix schenckii sensu lato and establish that there are two main species of Australian clinical isolates-S. stricto globosa. We also demonstrated southwest Western Australia contained genetically similar S. ss strains distinct from isolated in eastern northern states Australia. Some clustering by region noted for NSW, Queensland, Northern Territory. Phylogenetic analysis WGS data provided greater...
Early identification of MRSA by diagnostic medical microbiology laboratories enables improved antimicrobial choice and outcomes. The Cepheid Xpert® MRSA/SA BC test rapidly identifies Staphylococcus aureus bloodstream infections through spa gene detection methicillin resistance via mecA detection. Recent emergence S. with deletions in the has resulted false-negative results for this test, leading to misidentification organism, particularly ST45.To investigate prevalence ST45 New South Wales...
Mutations associated with linezolid resistance within the V domain of 23S rRNA are annotated using an Escherichia coli numbering system. The gene varies in length, nucleotide sequence, and copy number among bacterial species. Consequently, this system is not intuitive can lead to confusion when mutation sites being located whole-genome sequencing data. Using G2576T as example, we demonstrate difficulties E.
A marked genotype shift among vancomycin-resistant Enterococcus faecium (VREfm) from vanB to vanA in Australia between 2011 and 2015 is a well-known phenomenon. It hypothesized that this was caused by multiple independent clones emerging simultaneously different settings and/or regions.To gain insights into the circumstances surrounding VREfm one Australian hospital.The genomes of 69 isolates St George Hospital collected 2009 2018 were studied. An expansion ST80 noted following single...
Abstract Accumulating evidence supports the high prevalence of co-infections among Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) patients, and their potential to worsen clinical outcome COVID-19. However, there are few data on Southern Hemisphere populations, most studies date have investigated a narrow spectrum viruses using targeted qRT-PCR. Here we assessed respiratory viral SARS-CoV-2 patients in Australia, through virome characterization. Nasopharyngeal swabs 92...
ABSTRACT Here, we report the first draft genome sequence of Enterococcus thailandicus isolated from feces feedlot cattle in Southern Alberta.
Infections due to metallo-beta-lactamase (MBL)-producing organisms are becoming a significant problem, and antibiotic treatment options limited. Aztreonam inhibits MBLs, its use in combination with ceftazidime-avibactam (CAZ-AVI-AZT) inhibit other beta-lactamases shows promise.