A5080502041- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Amino Acid Enzymes and Metabolism
- Biomedical Research and Pathophysiology
- Neonatal Health and Biochemistry
- Erythrocyte Function and Pathophysiology
- Metabolomics and Mass Spectrometry Studies
- Hemoglobinopathies and Related Disorders
- Mitochondrial Function and Pathology
- Diet and metabolism studies
- Adipose Tissue and Metabolism
- Biochemical and Molecular Research
- Metabolism and Genetic Disorders
- RNA and protein synthesis mechanisms
- Prenatal Substance Exposure Effects
- Genomics and Phylogenetic Studies
- Cannabis and Cannabinoid Research
- Protein purification and stability
- Peptidase Inhibition and Analysis
- Signaling Pathways in Disease
- Cancer, Lipids, and Metabolism
- Digestive system and related health
- Neurogenetic and Muscular Disorders Research
- Diet, Metabolism, and Disease
- Nanopore and Nanochannel Transport Studies
Clarus Therapeutics (United States)
2019-2025
University of California, San Diego
2010-2022
Cornell University
2012
Scripps Research Institute
2009-2011
Capsid maturation with large-scale subunit reorganization occurs in virtually all viruses that use a motor to package nucleic acid into preformed particles. A variety of ensemble studies indicate the particles gain greater stability during this process, however, it is unknown which material properties fragile procapsids change. Using Atomic Force Microscopy-based nano-indentation, we study development mechanical bacteriophage HK97, λ -like phage maturation-induced morphological changes are...
Maternal obesity is proposed to alter the programming of metabolic systems in offspring, increasing risk for developing diseases; however, cellular mechanisms remain poorly understood. Here, we used a nonhuman primate model examine impact maternal Western-style diet (WSD) alone, or combination with (Ob/WSD), on fetal skeletal muscle metabolism studied early third trimester. We find that responds Ob/WSD by upregulating fatty acid metabolism, mitochondrial complex activity, and switches...
YG8R mice, a model of Friedreich’s ataxia, show amelioration the disease phenotype when transplanted with wild-type mouse hematopoietic stem and progenitor cells.
Metabolic dysfunction-associated steatotic liver disease (MASLD) is a multifactorial chronic that can progress to metabolic steatohepatitis (MASH) and fibrosis, ultimately leading cirrhosis hepatocellular carcinoma. Oxidative stress believed play an important role in the development of MASH. Small aminothiol compounds such as cysteamine its oxidized precursor, cystamine, are known pleiotropic exhibit relatively potent antioxidant other effects. Herein, we evaluate efficacy well two...
Various populations of cells are recruited to the heart after cardiac injury, but little is known about whether cardiomyocytes directly regulate repair. Using a murine model ischemic we demonstrate that play pivotal role in repair by regulating nucleotide metabolism and fates nonmyocytes. Cardiac injury induced expression ectonucleotidase ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), which hydrolyzes extracellular ATP form AMP. In response AMP, released adenine specific...
HK97 is a double-stranded DNA bacteriophage that undergoes dramatic conformational changes during viral capsid maturation and for which x-ray structures, at near atomic resolution, of multiple intermediate mature states are available. Both amide H/(2)H exchange crystallographic comparisons between the pre-expanded Prohead II particles expanded Head revealed quaternary interactions remain fixed throughout appear to maintain intercapsomer integrity all quasi- icosahedral 3-fold axes. These...
The acetyltransferase, E1a-binding protein (p300), is proposed to regulate various aspects of skeletal muscle development, metabolism, and mitochondrial function,viaits interaction with numerous transcriptional regulators other proteins. Remarkably, however, the contribution p300 function metabolism,in vivo, poorly understood. To address this, we used Cre-LoxP methodology generate mice muscle-specific knockout (mKO). mKO were indistinguishable from their wild-type/floxed littermates, no...
Alkaptonuria is an autosomal recessive disease involving a deficiency of the enzyme homogentisate dioxygenase, which involved in tyrosine degradation pathway. The enzymatic results high concentrations homogentisic acid (HGA), orthopedic and cardiac complications, among other symptoms. Nitisinone (NTBC) has been shown to effectively treat alkaptonuria by blocking conversion 4-hydroxyphenylpyruvate HGA, but there have concerns that using doses higher than about 2 mg/day could cause excessively...
Cystine determination is a critical biochemical test for the diagnosis and therapeutic monitoring of lysosomal storage disease cystinosis. The classical mixed-leukocyte cystine assay requires prompt specialized recovery/isolation following blood drawing, providing concentrations normalized to total protein from assorted types white cells, each with varying content.We present new workflow using immunomagnetic granulocyte purification, reference ranges established 47 patient 27 obligate...
The discovery of novel biomarkers has emerged as a critical need for therapeutic development in amyotrophic lateral sclerosis (ALS). For some subsets ALS, such the genetic superoxide dismutase 1 (SOD1) form, exciting new treatment strategies, antisense oligonucleotide-mediated (ASO-mediated) SOD1 silencing, are being tested clinical trials, so identification pharmacodynamic monitoring is essential. We identify increased levels 7-amino acid endogenous peptide cerebrospinal fluid (CSF) human...
Cannabis use is a component risk factor for the manifestation of schizophrenia. The biological effects cannabis include on epigenetic systems, immunological parameters, in addition to changes cannabinoid receptors 1 and 2, that may be associated with this risk. However, there has been limited study smoked these human peripheral blood cells. We analyzed two concentrations tetrahydrocannabinol (THC) vs. placebo lymphocytes subset participants who enrolled double-blind driving performance...
Cystinosis is an autosomal recessive lysosomal storage disorder caused by mutations in the CTNS gene encoding cystine transporter, cystinosin, and leading to multi-organ degeneration including kidney failure. A clinical trial for cystinosis ongoing test safety efficacy of transplantation autologous hematopoietic stem progenitor cells (HSPCs) ex vivo gene-modified introduce functional cDNA. Preclinical studies Ctns−/− mice previously showed that a single HSPC led significant tissue decrease...