A5101616930- Axon Guidance and Neuronal Signaling
- RNA modifications and cancer
- Virus-based gene therapy research
- S100 Proteins and Annexins
- Cancer Research and Treatments
- Cancer-related Molecular Pathways
- Cancer-related gene regulation
- Flavonoids in Medical Research
- Hippo pathway signaling and YAP/TAZ
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Photodynamic Therapy Research Studies
- Colorectal and Anal Carcinomas
- Plant-based Medicinal Research
- NF-κB Signaling Pathways
- Neurological Disorders and Treatments
- Cancer-related molecular mechanisms research
- Salivary Gland Tumors Diagnosis and Treatment
- PI3K/AKT/mTOR signaling in cancer
- RNA Interference and Gene Delivery
- Ocular Surface and Contact Lens
- CAR-T cell therapy research
- Cancer Mechanisms and Therapy
- Colorectal Cancer Treatments and Studies
- Nanoplatforms for cancer theranostics
Xiangya Hospital Central South University
2012-2024
Central South University
2012-2024
Abstract Overexpression of ANXA1 and EphA2 has been linked to various cancers both proteins have attracted considerable attention for the development new anticancer drugs. Here we report that competes with Cbl binding increases its stability by inhibiting Cbl-mediated ubiquitination degradation in nasopharyngeal carcinoma (NPC). Binding promoted NPC cell growth metastasis vitro vivo elevating levels increasing activity oncogenic signaling (pS897-EphA2). Expression was positively correlated...
Abstract HDAC7 plays a crucial role in cancers, and is the main drug target of several HDAC inhibitors. However, mechanism nasopharyngeal carcinoma (NPC) are still unclear. In this study, we observed that was significantly upregulated NPC tissues relative to normal mucosa (NNM) tissues, expression levels were positively correlated with progression negatively patient prognosis, knockdown dramatically inhibited vitro proliferation, migration, invasion cells, growth xenografts mice, indicating...
Abstract EphA2 is an important oncogenic protein and emerging drug target, but the role mechanism of ligand-independent phosphorylation at tyrosine 772 (pY772-EphA2) unclear. In this study, we established nasopharyngeal carcinoma (NPC) cell lines with stable expression exogenous EphA2-Y772A (phosphorylation inactivation) using endogenous EphA2-knockdown cells, observed that pY772A was responsible for EphA2-promoting NPC proliferation anchorage-independent in vivo growth mice....
Purpose: Aberrant expression and dysfunction of RSF1 has been reported in diverse human malignancies.However, its exact role nasopharyngeal carcinoma (NPC) remains unclear.Methods: The mRNA protein were assayed by qRT-PCR western blotting, their correlations with clinicopathological parameters patients NPC further analysed.Lentivirus mediated shRNA cDNA used to knockdown upregulate the RSF1.CCK8 assays flow cytometry applied monitor changes proliferation paclitaxel sensitivity caused...
EphA2 (EPH receptor A2) (erythropoietin‑producing hepatocellular tyrosine kinase subtype plays a crucial role in human cancers, and is promising target for the development of new anticancer drugs. In this study, we showed that interaction Annexin A1 (ANXA1) increased stability by inhibiting its proteasome degradation gastric cancer (GC) colon (CC) cells, amino acid residues 20‑30 28‑30 ANXA1 N terminal were responsible binding stabilizing EphA2. Based on EphA2, developed ANXA1‑derived 3...
The deregulation of microRNAs (miRNAs) is involved in the development and progression various types human malignancy. Previously, it has been suggested that miR‑214 an oncogenic role nasopharyngeal carcinoma. However, detailed molecular mechanisms remain to be elucidated. In present study, reverse transcription‑quantitative polymerase chain reaction western blot analysis were conducted determine relative mRNA protein levels, respectively. MTT assay was performed examine cell proliferation....
A heterozygous point mutation of p53 gene at codon 280 from AGA to ACA (R280T) frequently occurs in nasopharyngeal carcinoma (NPC) cell lines, and about 10% NPC tissues. However, the role this pathogenesis remains unclear. In study, we generated knockout (KO) lines CNE2 cells carrying R280T (p53-R280T) C666-1 wild-type by CRISPR-Cas9 editing system, found that KO p53-R280T significantly decreased proliferation increased apoptosis, whereas had opposite effects on apoptosis. Moreover,...
Nasopharyngeal carcinoma (NPC) is very common in Southern China and Southeast Asian countries. To explore a novel more effective approach to NPC therapy, combined strategy of suicide genes radiation was designed this study. Five gene expression cassettes, yeast CD, CD/UPRT, CDglyTK controlled by CMV, Egr-1 synthetic CMV-enhanced promoter (CE) were constructed an vector p11MS. The CNE-2 cells detected RT-PCR Western blot. cytotoxicity therapy analyzed MTT assay. An animal study which...
Colon cancer is among the most prevalent and fatal malignancies deadly type of tumor in world. Chemotherapy surgery are main choices treatment for patients. However, Resistance to chemotherapy remains one greats challenges mostly patients with metastatic lesions. This study will provide a comprehensive review different mechanisms colon resistance stem cells including, epithelial-mesenchymal transition (EMT), activation DNA damage checkpoints, hindrance over-expression antiapoptotic...
This study explored the 605 nm carboxyl of water-soluble quantum dots to assess practicability nasopharyngeal carcinoma marker EBNA1 antibody. We used and antigen in 1-(3-dimethylaminopropyl-3-2-ethylcarbonimine hydrochloride generate dot-labeled antigen. Gel imaging system showed that serum group 60 patients with had significant differences 30 cases normal adult serogroup brightness, Levene test two groups ELISA absorbance value was P<0.001, namely measured difference obvious. The...
Following the publication of this paper, it was drawn to Editors' attention by a concerned reader that certain cell apoptotic assay data shown in Figs. 2C and 4B were strikingly similar appearing different form other articles authors. Owing fact contentious above article had already been published elsewhere, or under consideration for publication, prior its submission <em>Molecular Medicine Reports</em>, Editor has decided paper should be retracted from Journal. The authors asked an...
<div>Abstract<p>Overexpression of ANXA1 and EphA2 has been linked to various cancers both proteins have attracted considerable attention for the development new anticancer drugs. Here we report that competes with Cbl binding increases its stability by inhibiting Cbl-mediated ubiquitination degradation in nasopharyngeal carcinoma (NPC). Binding promoted NPC cell growth metastasis <i>in vitro</i> vivo</i> elevating levels increasing activity oncogenic signaling...
<div>Abstract<p>Overexpression of ANXA1 and EphA2 has been linked to various cancers both proteins have attracted considerable attention for the development new anticancer drugs. Here we report that competes with Cbl binding increases its stability by inhibiting Cbl-mediated ubiquitination degradation in nasopharyngeal carcinoma (NPC). Binding promoted NPC cell growth metastasis <i>in vitro</i> vivo</i> elevating levels increasing activity oncogenic signaling...
<p>1.Supplementary Materials and Methods; Supplementary Table S1-4; Figure S1-8. S1. The clinicopathological characteristics of 184 patients with NPC. S2. primers used for the amplification three genes by qRT-PCR. S3. Correlations between two protein expression in S4. Univariate cox multivariate analyses prognostic factors overall disease-free survival. Identification ANXA1 Cbl as interactors EphA2 co-IP-mass spectrometry (MS) analysis. affects but not mRNA levels NPC cells. effect...
<p>1.Supplementary Materials and Methods; Supplementary Table S1-4; Figure S1-8. S1. The clinicopathological characteristics of 184 patients with NPC. S2. primers used for the amplification three genes by qRT-PCR. S3. Correlations between two protein expression in S4. Univariate cox multivariate analyses prognostic factors overall disease-free survival. Identification ANXA1 Cbl as interactors EphA2 co-IP-mass spectrometry (MS) analysis. affects but not mRNA levels NPC cells. effect...