A5068830420- Ocular Infections and Treatments
- Herpesvirus Infections and Treatments
- Virus-based gene therapy research
- Intraocular Surgery and Lenses
- Ocular Surface and Contact Lens
- Cytomegalovirus and herpesvirus research
- Bacterial biofilms and quorum sensing
- Ocular Diseases and Behçet’s Syndrome
- Antibiotics Pharmacokinetics and Efficacy
- Corneal Surgery and Treatments
- Antibiotic Resistance in Bacteria
- Antimicrobial Resistance in Staphylococcus
- Retinal and Optic Conditions
- Bacterial Identification and Susceptibility Testing
- Acne and Rosacea Treatments and Effects
- Antimicrobial Peptides and Activities
- Vibrio bacteria research studies
- Glaucoma and retinal disorders
- Spectroscopy Techniques in Biomedical and Chemical Research
- Viral Infectious Diseases and Gene Expression in Insects
- Corneal surgery and disorders
- Toxoplasma gondii Research Studies
- interferon and immune responses
- Animal Virus Infections Studies
- Healthcare and Venom Research
University of Pittsburgh
2015-2024
University of Pittsburgh Medical Center
2011-2022
Eye and Ear Foundation
2009-2022
Campbell Foundation
2019
Prevent Blindness
2014-2018
Visual Sciences (United States)
2018
NovaBay Pharmaceuticals (United States)
2018
United States Patent and Trademark Office
2018
Research to Prevent Blindness
2001-2018
Eye Center
2010
Purpose: This study determined whether LL-37 (cathelicidin) is expressed by conjunctival and corneal epithelia as part of ocular host defense. The antimicrobial activity was also assessed in vitro against Pseudomonas aeruginosa (PA), Staphylococcus aureus (SA), epidermidis (SE), herpes simplex virus type 1 (HSV-1), adenovirus (Ad). Methods: Expression LL-37/hCAP 18 mRNA protein reverse transcription–polymerase chain reaction (RT-PCR) immunoblotting, respectively, scraped human epithelium...
Ocular infections are a leading cause of vision loss. It has been previously suggested that predatory prokaryotes might be used as live antibiotics to control infections. In this study, Pseudomonas aeruginosa and Serratia marcescens ocular isolates were exposed the bacteria Micavibrio aeruginosavorus Bdellovibrio bacteriovorus. All tested S. susceptible predation by B. bacteriovorus strains 109J HD100. Seven 10 P. with 80% being attacked M. aeruginosavorus. 19 found sensitive at least one...
Purpose: Brilacidin (BRI), a novel defensin mimetic, was evaluated as an ocular anti-infective. Methods: In vitro: Potency based on MIC90s compared for 50 Staphylococcus aureus (SA), epidermidis (SE), and 25 each of Streptococcus pneumonia (SP), viridans (SV), Moraxella (MS), Haemophilus influenzae (HI), Pseudomonas aeruginosa (PA), Serratia marcescens (SM). vivo: Using established methods, toxicity graded with Draize testing. For efficacy testing, both corneas 24 rabbits were infected...
Purpose: The definitive identification of ocular pathogens optimizes effective treatment. Although the types are known; there is less information on prevalence causative infections including viruses, fungi, and protozoa, which focus this retrospective laboratory review. Methods: Data used for certification were reviewed detection bacteria, from patients with infectious keratitis, endophthalmitis, conjunctivitis. main outcome parameter was laboratory-positive infection. Results: distribution...
Medical textiles have a need for repellency to body fluids such as blood, urine, or sweat that may contain infectious vectors contaminate surfaces and spread other individuals. Similarly, viral has yet be demonstrated long-term mechanical durability is major challenge. In this work, we demonstrate simple, durable, scalable coating on nonwoven polypropylene textile both superhemophobic antivirofouling. The treatment consists of polytetrafluoroethylene (PTFE) nanoparticles in solvent thermally...
Recent studies with mice have demonstrated abundant RNA transcripts which are complementary (antisense) to the herpes alpha gene ICP0 in latently infected ganglia. We investigated situation unselected human trigeminal Strand-specific 2.7-kilobase simplex virus type 1 (HSV-1) probes were prepared, and their sense was determined productively cells. Although situ hybridization antisense nuclei of neurons 46% ganglia, messenger not found any conclude that HSV-1 is present humans during...
Human adenoviruses (HAdVs) are uniquely important "model organisms" as they have been used to elucidate fundamental biological processes, recognized complex pathogens, and remedies for human health. As HAdVs may effect asymptomatic or mild severe symptomatic disease upon their infection of respiratory, ocular, gastrointestinal, genitourinary systems. High-resolution genomic data enhanced the understanding HAdV epidemiology, with recombination an major pathway in molecular evolution genesis...
To evaluate the effects of Pred Forte (prednisolone acetate; Allergan Pharmaceutical, Irvine, Calif) on replication different adenoviral serotypes in vitro and adenovirus type 5/New Zealand rabbit ocular model.The 50% inhibitory doses its components were determined for common serotypes. The continuous topical treatment with 18 days evaluated (eg, conjunctivitis, subepithelial immune infiltrates, serial viral titers) model.Pred prednisolone acetate inhibited adenoviruses 1, 5, 8, 19 vitro. In...
The epithelium provides a crucial barrier to infection, and its integrity requires efficient wound healing. Bacterial cells secretomes from subset of tested species bacteria inhibited human porcine corneal epithelial cell migration in vitro ex vivo. Secretomes 95% Serratia marcescens, 71% Pseudomonas aeruginosa, 29% Staphylococcus aureus strains, other bacterial migration. Migration foreskin fibroblasts was also by S. marcescens indicating that the effect is not cornea specific. Transposon...
Abstract Given the increasing emergence of antimicrobial resistant microbes and near absent development new antibiotic classes, innovative therapeutic approaches to address this global problem are necessary. The use predatory bacteria, bacteria that prey upon other is gaining interest as an “out box” treatment for multidrug pathogenic bacterial infections. Before a agent used treat infections, it must be tested safety. goal study was test tolerability on ocular surface using in vitro vivo...
This work demonstrates a coal-derived functionalized nano-graphene oxide coating applied to fabrics that exhibits antiviral properties even after mechanical abrasion or bleach washing. Nano-graphene is chemically exfoliated from low cost coal and with octadecylamine render repellency properties. The polyethylene terephthalate (PET) fabric wet etching which roughens the microfiber surface for better adhesion liquid repellency. An additional polydimethylsiloxane (PDMS) layer on top of further...
Cefazolin/tobramycin, cefuroxime/gentamicin, and moxifloxacin were compared using bacterial keratitis isolates to determine whether empiric therapy constituted optimal antibacterial treatment.Based on percent incidence of corneal infection, 27 Staphylococcus aureus, 16 Pseudomonas aeruginosa, 10 Serratia marcescens, 4 Moraxella lacunata, 3 Haemophilus influenzae, 9 coagulase-negative staphylococci, 7 Streptococcus viridans, 6 pneumoniae, assorted Gram-positive isolates, 11 Gram-negative...