Mitochondrial oxidative damage is hypothesized to contribute the pathogenesis of chronic cholestatic liver diseases. Melatonin, an indolamine synthesized in pineal gland, shows a wide range physiological functions, and under clinical investigation for expanded applications. Melatonin has demonstrated efficient protective effects against various types system. This study investigates melatonin pretreatment on glycochenodeoxycholic acid (GCDCA)-induced hepatotoxicity elucidates potential...

Liver ischemia and reperfusion (I/R) injury is characterized by defective liver autophagy accompanied alterations to the endogenous defense system. Dihydromyricetin (DHM) a natural flavonoid that demonstrates wide range of physiological functions, has been implicated as regulator autophagy. This study investigates protective effects DHM pretreatment on caused ischemia/reperfusion elucidates potential mechanism DHM-mediated protection. Mice were subjected 60 minutes followed 5 hours...

Clusterin (CLU) is expressed in a wide variety of human tissues and fluids. Overexpression cytoplasmic clusterin (sCLU) has been implicated cancer development progression. The aim the present study to evaluate association sCLU overexpression with clinicopathological features gastric carcinomas (GC).We constructed tissue microarray containing 173 primary 70 paired non-neoplastic mucosa specimens. expression was studied by immunohistochemistry. correlations between features, p53 abnormality,...

The present study was designed to determine the significance of DNA topoisomerase IIa (TopoIIα) and Ki67 in hepatocellular carcinoma cells (HCCs). included 353 patients with HCC. association clinicopathological data expression TopoIIα by immunohistochemistry analyzed χ2 test. Cox multivariate proportional hazards regression analysis Kaplan-Meier were performed all variables derive risk estimates associated overall survival (OS)/recurrence-free (RFS) control for confounders. detected nuclei...

Early detection and prognostic prediction of hepatocellular carcinoma (HCC) remain a great challenge. In this study, we explored the role diagnostic significance stanniocalcin 2 (STC2), recently identified as secretory protein, in HCC.STC2 mRNA protein HCC tissues were examined by qRT-PCR immunohistochemistry. The regulatory growth STC2 was evaluated vitro vivo. Serum levels determined patients compared to those with liver cirrhosis (LC) normal controls (NC). difference between groups...

Food grade titanium dioxide (TiO2) containing nanofractions, is commonly applied to whiten and brighten food products, which put consumers under health risks of ingesting TiO2 nanoparticles (NPs). Although the oral toxicity TiO2-NPs has been evaluated in several studies, gaps knowledge exist regarding interactions between NPs components. Therefore, this study aimed estimate influence on nutrient absorption metabolism through an situ intestinal loop experiment conducted adult Sprague Dawley...

Abstract Cathepsin A (CTSA) is a lysosomal protease that regulates galactoside metabolism. The previous study has shown CTSA abnormally expressed in various types of cancer. However, rarely the addressed role hepatocellular carcinoma (HCC) and its prognostic value. To clinical value potential function HCC, datasets from Cancer Genome Atlas (TCGA) database 136 HCC patient cohort were analyzed. expression was found to be significantly higher patients compared with normal liver tissues, which...

Small nuclear ribonucleoprotein Sm D1 (SNRPD1), one of the crucial genes encoding core spliceosome components, was abnormally highly expressed in multiple types tumors. In this study, we investigated diagnostic and prognostic significance SNRPD1 hepatocellular carcinoma (HCC). The investigation datasets from GEO TCGA databases revealed that expression HCC significantly higher than adjacent normal liver tissues, which validated by immunohistochemistry (IHC). Both GO, KEGG analysis showed...

Mitochondrial impairment is hypothesized to contribute the pathogenesis of chronic cholestatic liver diseases. Mitofusin 2 (Mfn2) regulates mitochondrial morphology and signaling involved in development numerous mitochondrial-related diseases; however, a functional role for Mfn2 cholestasis which characterized by increased levels toxic bile acids remain unknown. Therefore, aims this study were evaluate expression samples from patients with extrahepatic investigate during acid induced injury...

Chaperonin containing TCP1 subunit 7 (CCT7) regulates the expression of many tumor-related proteins. We investigated diagnostic and prognostic value CCT7 for hepatocellular carcinoma (HCC). In datasets from The Cancer Genome Atlas Gene Expression Omnibus, mRNA levels were greater in HCC tissues than adjacent normal tissues, these results validated using immunohistochemistry. patients with early-stage disease low alpha-fetoprotein expression, was still higher tissues. Receiver operating...

Increasing evidence has displayed the vital influence of lncRNA in tumorigenesis and chemoresistance cancer treatment. This study investigated function PTOV1-AS1 hepatocellular carcinoma (HCC) its role sorafenib resistance. The relative expression miRNA was measured by RT-qPCR. cellular activities including cell proliferation invasion were explored CCK-8 Transwell assays. Bioinformatics analysis dual-luciferase reporter assay used to predict targeting PTOV1-AS1. levels higher HCC tissues...

Growing evidence has shown that coffee consumption is inversely related with the risk of hepatocellular carcinoma. It suggested caffeine maintains strong antioxidative activity. With this property, intake may lead to inhibition cell proliferation liver cancer cells; also, some compounds contained in can reduce genotoxicity aflatoxin B1 vitro and lower damage caused by carcinogens. A computerized search was performed PubMed identify relevant articles published before August 2015. Eleven...

The platelet to lymphocyte ratio (PLR) is a readily available cancer biomarker but its prognostic value for patients with resected primary hepatocellular carcinoma (HCC) uncertain. Thus, we investigated the relationship between PLR and survival of HCC in Fujian area, high occurrence area China.This retrospective study included 337 who underwent surgical removal liver 2004 2012 Fuzhou General Hospital Fujian, China. Pre-operative peripheral blood was measured by counts divided counts....

Rho GDP dissociation inhibitor (GDI) beta, (RhoGDI2), has been identified as a proto-oncogene that is upregulated in human cancers, but the role of RhoGDI2 hepatocellular carcinoma (HCC) remains unclear. In present study, we investigated expression level HCC tissues and function cell growth metastasis. We examined mRNA 64 sets tissue their adjacent nontumor counterparts using quantitative real-time polymerase chain reaction. vitro proliferation invasion assays were conducted to determine...

Antigen Ki-67 (Ki67) is a documented tumor biomarker known to be aberrantly expressed in many human cancers. However, our knowledge, its prognostic value for surgically resected hepatocellular carcinoma (HCC) high incidence areas of China has not been described.We retrospectively investigated 339 patients with HCC who underwent surgery remove the primary cancer at one center Fujian, China, an area from 2004 2012. Immunohistochemistry (IHC) was used quantify Ki67 expression (RHCC) tissues....

Heterogeneous ribonucleoproteinA1 (hnRNPA1) is a documented tumor biomarker known to be aberrantly expressed in number of types human cancer. However, the best our knowledge, its prognostic value for surgically resected HCC (RHCC) high incidence areas China has not been described; association between hnRNPA1 expression, pre-operative neutrophil lymphocyte ratio (NLR) and platelet (PLR) also understood. In present study, expression was retrospectively measured two independent cohorts patients...

As powerful regulatory factors, microRNAs (miRNAs) are involved in tumor progression. The current research aimed to excavate the prognostic significance and potential mechanisms of miR-652-3p hepatocellular carcinoma (HCC). Expression HCC tissues cells was exposed by Quantitative real-time polymerase chain reaction (RT-qPCR) assay, we found that elevated than control group (P < 0.05). Then, relationship between levels clinical characteristics obtained from Chi-square test. Kaplan-Meier...

To explore the benefit and safety of transarterial chemoembolization (TACE) in combination with sorafenib patients recurrent hepatocellular carcinoma (HCC) after orthotopic liver transplantation (OLT).In this multi-center retrospective study, 106 HCC OLT were included. Fifty-two treated TACE plus (TS group) 54 alone (TC group). Primary secondary endpoints including overall survival (OS) progression-free (PFS), assessed.The median OS (17 vs 10 months, P=0.035) PFS (12 6 P=0.004) TS group...

Hepatocellular carcinoma (HCC) remains imposing an enormous economic and healthcare burden worldwide. In this present study, we constructed validated a novel autophagy-related gene signature to predict the recurrence of HCC patients. A total 29 differentially expressed genes were identified. five-gene (CLN3, HGF, TRIM22, SNRPD1, SNRPE) was for prediction. Patients in high-risk groups exhibited significantly poor prognosis compared with low-risk patients both training set (GSE14520 dataset)...

Background/Aims: Increasing evidence has indicated that Forkhead box protein C2 (FOXC2) plays an important role in carcinogenesis. However, the expression and of FOXC2 hepatocellular carcinoma (HCC) have not been extensively studied. Methods: was analyzed by quantitative real-time polymerase chain reaction, Western blot analysis immunohistochemistry HCC tissue cells. The relationship between patient clinical significance survival were assessed Pearson’s correlation Kaplan-Meier analysis,...