A5030460457- Estrogen and related hormone effects
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sperm and Testicular Function
- Hormonal and reproductive studies
- Reproductive Biology and Fertility
- Hypothalamic control of reproductive hormones
- Histone Deacetylase Inhibitors Research
- Retinoids in leukemia and cellular processes
- Ovarian function and disorders
- Reproductive System and Pregnancy
- Hormonal Regulation and Hypertension
- Stress Responses and Cortisol
- Cholesterol and Lipid Metabolism
- Ovarian cancer diagnosis and treatment
- Animal Genetics and Reproduction
- Sirtuins and Resveratrol in Medicine
- DNA and Nucleic Acid Chemistry
- COVID-19 Clinical Research Studies
- Adipose Tissue and Metabolism
- Biochemical effects in animals
- Epigenetics and DNA Methylation
- Neuroscience and Neuropharmacology Research
- Peroxisome Proliferator-Activated Receptors
- Reproductive Physiology in Livestock
- Growth Hormone and Insulin-like Growth Factors
Texas Tech University
2014-2025
Texas Tech University Health Sciences Center
2014-2025
Magna Graecia University
2021
Consejo Nacional de Investigaciones Científicas y Técnicas
2003
University of Buenos Aires
2003
Experimental Medicine and Biology Institute
2003
University of Geneva
2002
Nagoya University
2002
University of Turku
1997-2002
University of New Hampshire
2002
Abstract The mitochondrial phosphoprotein, the steroidogenic acute regulatory (StAR) protein, is an essential component in regulation of steroid biosynthesis adrenal and gonadal cells through cAMP-dependent pathways. In many cases transcriptional induction by cAMP mediated interaction a response-element binding protein (CREB) family member with consensus response element (CRE; 5′-TGACGTCA-3′) found promoter target genes. present investigation was carried out to determine whether CRE-binding...
Recent data implicate locally produced steroids, termed neurosteroids, as regulators of neuronal function. Adrenal and gonadal steroidogenesis is controlled by changes in the steroidogenic acute regulatory protein (StAR); however, little known about regulation neurosteroid production. We now demonstrate unequivocally that StAR mRNA are expressed within glia neurons discrete regions mouse brain, glial expression inducible. Consistent with a role <i>de novo</i>neurosteroidogenesis, colocalizes...
Growth factors are known to play diverse roles in steroidogenesis, a process regulated by the mitochondrial steroidogenic acute regulatory (StAR) protein. The mechanism of action one such growth factor, IGF-I, was investigated mouse Leydig tumor (mLTC-1) cells determine its potential role regulation StAR expression. mLTC-1 treated with IGF-I demonstrated temporal and concentration-dependent increases expression steroid synthesis. However, had no effect on cytochrome P450 side-chain cleavage...
Mitogen-activated protein kinases (MAPKs) comprise a family of serine/threonine that are activated by large variety extracellular stimuli and play integral roles in controlling many cellular processes, from the cell surface to nucleus. The MAPK includes four distinct cascades, is, signal-regulated kinase 1/2 (ERK1/2), p38 MAPK, c-Jun N-terminal or stress-activated kinase, ERK5. These MAPKs essentially operated through three-tiered consecutive phosphorylation events catalyzed MAPK. lie...
In the regulation of steroid biosynthesis, a process mediated by steroidogenic acute regulatory (StAR) protein, both cAMP-dependent and -independent pathways are involved. While events represent, far, most robust increase in synthesis well established, knowledge regarding cAMP-independent mechanisms is lacking. The present investigation was designed to elucidate potential involvement latter regulating StAR expression steroidogenesis mouse Leydig tumor cells (mLTC-1 cells). Treatment mLTC-1...
Significance Many diseases result from genetic mutations that cause protein misfolding. Medical treatments often address the symptoms, but do not correct underlying etiology. This study illustrates proof of principle a disease caused by misfolded cell surface receptor can be corrected with pharmacoperone, unique class target-specific drugs assist folding.
Abstract Human skin has the ability to synthesize glucocorticoids de novo from cholesterol or steroid intermediates of systemic origin. By interacting with glucocorticoid receptors, they regulate immune functions as well and phenotype epidermal, dermal adnexal compartments. Most biochemical (enzyme transporter activities) regulatory (neuropeptides mediated activation c AMP protein kinase A dependent pathways) principles steroidogenesis in are similar those operating classical steroidogenic...
Recently, we demonstrated that triiodothyronine (T(3)) stimulated steroid hormone biosynthesis and steroidogenic acute regulatory (StAR) protein expression in mLTC-1 mouse Leydig tumor cells through the mediation of factor 1 (SF-1). We now report a dual response mechanism T(3) on steroidogenesis StAR expression, LH receptor (LHR) binding cells. acutely (8 h), induced 260% increase messenger RNA (mRNA) over basal level which was coincident with an progesterone (P) production. In contrast,...
Using a mouse Leydig tumor cell line, we explored the mechanisms involved in thyroid hormone-induced steroidogenic acute regulatory (StAR) protein gene expression, and steroidogenesis. Triiodothyronine (T3) induced ∼3.6-fold increase steady-state level of StAR mRNA which paralleled with those steroid response (∼4.0-fold), as monitored by quantitative reverse transcriptase-polymerase chain reaction assay progesterone production, respectively. The T3-stimulated production was effectively...
The steroidogenic acute regulatory (StAR) protein plays a central role in the regulation of steroid biosynthesis. While steroidogenesis is influenced by many processes, their modes actions, few cases, remain obscure. In this study, we explored mechanism action one such signaling pathway, extracellular signal-regulated kinase 1/2 (ERK1/2), regulating StAR expression and conjunction with A (PKA) C (PKC) pathways. Using MA-10 mouse Leydig tumor cells, demonstrate that activation PKC PKA...
Abstract Transcriptional regulation of the steroidogenic acute regulatory ( StAR ) protein gene by cAMP-dependent mechanisms occurs in absence a consensus cAMP-response element (CRE; TGACGTCA) and is mediated several sequence-specific transcription factors. We previously identified three CRE-like sites (within −151/−1 bp cAMP-responsive region mouse gene), which CRE2 site overlaps with an activator protein-1 (AP-1) motif (TGACTGA, designated as CRE2/AP-1) that can bind both CRE AP-1...
The protein kinase C (PKC) signaling pathway plays integral roles in the expression of steroidogenic acute regulatory (StAR) that regulates steroid biosynthesis cells. PKC can modulate activity cAMP/protein A involved steroidogenesis; however, its mechanism remains obscure. In present study, we demonstrate activation pathway, by phorbol 12-myristate 13-acetate (PMA), was capable potentiating dibutyryl cAMP [(Bu)2cAMP]-stimulated StAR expression, phosphorylation, and progesterone synthesis...