A5063719360- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Developmental Biology and Gene Regulation
- Pluripotent Stem Cells Research
- Renal and related cancers
- Reproductive Biology and Fertility
- Genomics and Chromatin Dynamics
- Cancer-related molecular mechanisms research
- NF-κB Signaling Pathways
- DNA Repair Mechanisms
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Cancer-related Molecular Pathways
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Immunotherapy and Immune Responses
- Sperm and Testicular Function
- Plant biochemistry and biosynthesis
- Adipose Tissue and Metabolism
- Genetics and Neurodevelopmental Disorders
- Bone Metabolism and Diseases
- Osteoarthritis Treatment and Mechanisms
- Congenital heart defects research
- Cancer Immunotherapy and Biomarkers
Kunming University of Science and Technology
2024
Zigong First People's Hospital
2023
Southwest Medical University
2021-2022
Shanghai Cell Therapy Research Institute
2022
Anhui Agricultural University
2022
First Affiliated Hospital of Sichuan Medical University
2021
University of Hong Kong
2020
The University of Texas MD Anderson Cancer Center
2007-2019
Northwest University
2015
Baylor College of Medicine
2011
Chondrogenesis is a multistep process that essential for endochondral bone formation. Previous results have indicated role β-catenin and Wnt signaling in this pathway. Here we show the existence of physical functional interactions between Sox9, transcription factor required successive steps chondrogenesis. In vivo, either overexpression Sox9 or inactivation β -catenin chondrocytes mouse embryos produces similar phenotype dwarfism with decreased chondrocyte proliferation, delayed hypertrophic...
The transcription factor Sox9 is expressed in all chondroprogenitors and has an essential role chondrogenesis. also other tissues, including central nervous system, neural crest, intestine, pancreas, testis, endocardial cushions, plays a crucial cell proliferation differentiation several of these tissues. To determine the fate -expressing cells during mouse embryogenesis, we generated mice which Cre recombinase gene preceded by internal ribosome entry site was inserted into 3′ untranslated...
In humans, SOX9 heterozygous mutations cause the severe skeletal dysmorphology syndrome campomelic dysplasia. Except for clinical descriptions, little is known about pathogenesis of this disease. We have generated Sox9 mutant mice that phenocopy most abnormalities syndrome. The +/− died perinatally with cleft palate, as well hypoplasia and bending many structures derived from cartilage precursors. embryonic day (E)14.5 embryos, radius, ulna, tibia cartilages was already prominent. E12.5...
Transition nuclear proteins (TPs), the major found in chromatin of condensing spermatids, are believed to be important for histone displacement and condensation during mammalian spermatogenesis. We generated mice lacking TP, TP1, by targeted deletion Tnp1 gene mouse embryonic stem cells. Surprisingly, testis weights sperm production were normal mutant mice, only subtle abnormalities observed morphology. Electron microscopy revealed large rod-like structures step 13 contrast fine fibrils wild...
Mutation of the transcription factor and tumor suppressor gene WT1 results in a range genitourinary anomalies humans, including 46,XY gonadal dysgenesis, indicating that plays critical role sex determination. However, because knockout Wt1 mice apoptosis genital ridge, it is unknown whether required for testis development after initial steps To address this question, we generated mouse strain carrying conditional allele ablated function specifically Sertoli cells by embryonic day 14.5,...
During mammalian spermiogenesis, major restructuring of chromatin takes place. In the mouse, histones are replaced by transition proteins, TP1 and TP2, which in turn protamines, P1 P2. To investigate role we generated mice with a targeted deletion its gene, Tnp2. Spermatogenesis Tnp2 null was almost normal, testis weights epididymal sperm counts being unaffected. The only abnormality testicular histology slight increase retention stage IX to XI tubules. Epididymal from Tnp2-null showed an...
Sox9 expression defines cell progenitors in a variety of tissues during mouse embryogenesis. To establish genetic tool for cell-lineage tracing and gene-function analysis, we generated mice which the CreERT2 gene was targeted to endogenous locus. In Sox9(CreERT2/+) ;R26R embryos, tamoxifen activated Cre recombinase exclusively Sox9-expressing tissues. determine suitability this line developmental stage-specific recombination, investigated cellular origins cruciate ligaments knee joint limb...
Abstract The function of cartilage in the adult is dependent on a host regulatory molecules such as growth factors, extracellular matrix, enzymes, signaling molecules, and transcription factors. However, germline mutations some genes that are expressed lead to embryonic or perinatal lethality. To examine these other postnatally, we have generated targeted mouse by homologous recombination “knocks in” inducible Cre recombinase construct, CreERT2, 3′ untranslated region endogenous aggrecan...
The success of postnatal uterine morphogenesis dictates, in part, the embryotrophic potential and functional capacity adult uterus. definitive role Wnt7a development function requires a conditional knockout, because global deletion disrupts müllerian duct patterning, specification, cell fate fetus. Wnt7a-null uterus appears to be posteriorized developmental defects embryo, as evidenced by stratified luminal epithelium that is normally found vagina presence short uncoiled oviducts. To...
X-linked heterotaxy (HTX1) is a rare developmental disorder characterized by disturbances in embryonic laterality and other midline field defects. HTX1 results from mutations ZIC3, member of the GLI transcription factor superfamily. A targeted deletion murine Zic3 locus has been created to investigate its function interactions with molecular components left-right axis pathway. Embryonic lethality seen approximately 50% null mice an additional 30% perinatal period. Null embryos have defects...
USF1 and USF2 are ubiquitously expressed transcription factors implicated as antagonists of the c-Myc protooncoprotein in control cellular proliferation. To determine biological role USF proteins, mutant mice were generated by homologous recombination embryonic stem cells. USF1-null viable fertile, with only slight behavioral abnormalities. However, these contained elevated levels USF2, which may compensate for absence USF1. In contrast, USF2-null reduced displayed an obvious growth defect:...
The Xenopus cerberus gene encodes a secreted factor that is expressed in the anterior endomesoderm of gastrula stage embryos and can induce formation ectopic heads when its mRNA injected into [Bouwmeester, T., Kim, S., Lu, B. & De Robertis, E. M. (1996) Nature (London) 382, 595–601]. Here we describe existence -related gene, Cerr1 , mouse. putative protein 48% identical to over 110-amino acid region. Analysis mouse interspecific backcross panel demonstrated mapped central portion...
Bone mass is maintained by continuous remodeling through repeated cycles of bone resorption osteoclasts and formation osteoblasts. This process regulated many systemic local factors.We identified collagen triple helix repeat containing-1 (Cthrc1) as a downstream target morphogenetic protein-2 (BMP2) in osteochondroprogenitor-like cells PCR-based suppression subtractive hybridization followed differential hybridization, found that Cthrc1 was expressed tissues vivo. To investigate the role...
Significance Many diseases result from genetic mutations that cause protein misfolding. Medical treatments often address the symptoms, but do not correct underlying etiology. This study illustrates proof of principle a disease caused by misfolded cell surface receptor can be corrected with pharmacoperone, unique class target-specific drugs assist folding.