A5077583058- Probiotics and Fermented Foods
- Listeria monocytogenes in Food Safety
- Bacterial biofilms and quorum sensing
- Heat shock proteins research
- Autophagy in Disease and Therapy
- Enzyme Structure and Function
- Essential Oils and Antimicrobial Activity
- Microbial Inactivation Methods
- Gut microbiota and health
- Biochemical Analysis and Sensing Techniques
- Antimicrobial Peptides and Activities
- Protein Structure and Dynamics
- Meat and Animal Product Quality
- Metabolomics and Mass Spectrometry Studies
- Microbial Metabolism and Applications
- Immune cells in cancer
- Protein Hydrolysis and Bioactive Peptides
- Bacterial Infections and Vaccines
- Clostridium difficile and Clostridium perfringens research
- Fatty Acid Research and Health
- Tea Polyphenols and Effects
- Proteins in Food Systems
- Extracellular vesicles in disease
- Vibrio bacteria research studies
- Transgenic Plants and Applications
Institut Agro Dijon
2014-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2024
Procédés Alimentaires et Microbiologiques
2016-2024
Université Bourgogne Franche-Comté
2019-2023
Université de Bourgogne
2008-2020
UCLouvain
2011
Health Canada
2007
Agriculture and Agri-Food Canada
2007
Genetics and Improvement of Fruit and Vegetables
2001
Mitochondria have emerged as key actors of innate and adaptive immunity. Mitophagy has a pivotal role in cell homeostasis, but its contribution to macrophage functions host defense remains be delineated. Here, we showed that lipopolysaccharide (LPS) combination with IFN-γ inhibited PINK1-dependent mitophagy macrophages through STAT1-dependent activation the inflammatory caspases 1 11. In addition, demonstrated inhibition triggered classical mitochondrial ROS-dependent manner. murine model...
ABSTRACT Listeria monocytogenes is a facultative intracellular pathogen thought to be widely distributed in the environment. We investigated prevalence and characteristics of L. isolates from surface waters derived catchments within South Nation River watershed (Ontario, Canada). This dominated by urban rural development, livestock crop production, wildlife habitats. From June November 2005, total 314 water samples were collected biweekly 22 discrete sampling sites characterized various...
ABSTRACT In this study, we investigated the agrBDCA operon in pathogenic bacterium Listeria monocytogenes EGD-e. In-frame deletion of agrA and agrD resulted an altered adherence biofilm formation on abiotic surfaces, suggesting involvement agr system L. during early stages formation. Real-time PCR experiments indicated that transcript levels depended stage development, since were lower after initial attachment period than growth, whereas transcription planktonic growth was not phase...
Listeria monocytogenes is a food pathogen that can attach on most of the surfaces encountered in industry. Biofilms are three-dimensional microbial structures facilitate persistence pathogens surfaces, their resistance toward antimicrobials, and final contamination processed goods. So far, little known about structural dynamics L. biofilm formation its regulation. The aims this study were, by combining genetics time-lapse laser-scanning confocal microscopy (LSCM), (i) to characterize EGD-e...
The predominant form of life for microorganisms in their natural habitats is the biofilm mode growth. adherence and colonization probiotic bacteria are considered as essential factors immunoregulatory function host. Here, we show that Lactobacillus casei ATCC334 adheres to colonizes gut zebrafish larvae. abundance pro-inflammatory cytokines recruitment macrophages were low when inflammation was induced probiotic-fed animals, suggesting these have anti-inflammatory properties. We treated...
Abstract In this study, we show that calcium pectinate beads (CPB) allow the formation of 20 µm spherical microcolonies probiotic bacteria Lacticaseibacillus paracasei (formerly designated as Lactobacillus ) ATCC334 with a high cell density, reaching more than 10 log (CFU/g). The within these are well structured and adhere to three-dimensional network made calcium-pectinate through synthesis extracellular polymeric substances (EPS) thus display biofilm-like phenotype, an attractive property...
Abstract Biofilms represent a major concern in the food industry and healthcare. The use of probiotic bacteria their derivatives as an alternative to conventional treatments fight biofilm development is promising option that has provided convincing results last decades. Recently, membrane vesicles (MVs) produced by probiotics have generated considerable interest due diversity roles they been associated with. However, antimicrobial activity MVs remains be studied. In this work, we showed...
Listeria monocytogenes is a facultative intracellular pathogen that can be carried asymptomatically in various animals and shed feces. We investigated the prevalence characteristics of L. isolated from livestock, wildlife, human potential sources contamination 2 areas Ontario, Canada. From February 2003 to November 2005, total 268 fecal samples were collected different animals. was using selective enrichment, isolation, confirmation procedures, 15 (6%) yielded isolation 84 confirmed strains....
Lactobacillus plantarum ctsR was characterized. found to be cotranscribed with clpC and induced in response various abiotic stresses. deletion conferred a heat-sensitive phenotype peculiar cell morphological features. The transcriptional pattern of putative CtsR regulon genes examined the Delta mutant. Direct CtsR-dependent regulation demonstrated by DNA-binding assays using recombinant promoters ctsR-clpC operon hsp1.
The last few decades have demonstrated that membrane vesicles (MVs) produced by microorganisms can a wide variety of functions. This diversity places MVs at the crossroads major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage and pathogenesis.
Omega-3 (n-3) polyunsaturated fatty acids (PUFAs), particularly docosahexaenoic acid (DHA), are required for the structure and function of retina. Several observational studies indicate that consumption a diet with relatively high levels n-3 PUFAs, such as those provided by fish oils, has protective effect against development age-related macular degeneration. Given accumulating evidence showing role gut microbiota in regulating retinal physiology host lipid metabolism, we evaluated potential...
Driving kombucha fermentation through microbial consortia remains based on scarce scientific elements, especially regarding the consumption of nitrogenous nutrients. This study aimed at analyzing quantitatively proteins and free amino nitrogen (FAN) concentrations, qualitatively soluble profiles during a 12-day production process. The effect interactions was evaluated by comparing growth previously isolated major microorganisms in monoculture cocultures to original kombucha. Protein FAN...
The ability of the small Hsp (heat-shock protein) Lo18 from Oenococcus oeni to modulate membrane fluidity liposomes or reduce thermal aggregation proteins was studied as a function pH in range 5-9. We have determined by size-exclusion chromatography and analytical ultracentrifugation that assembles essentially 16-mer at acidic pH. Its quaternary structure evolves mixture lower molecular mass oligomers probably dynamic equilibrium when increases. best activities are observed 7 particle...
Autophagy is a lysosomal degradation process that contributes to host immunity by eliminating invasive pathogens and the modulating inflammatory response. Several infectious immune disorders are associated with autophagy defects, suggesting stimulation of in these diseases should be beneficial. Here, we show resveratrol able boost xenophagy, selective form target bacteria. We demonstrated promotes vitro autophagy-dependent clearance intracellular bacteria intestinal epithelial cells...
Small heat shock proteins (sHSPs) are ubiquitous, low molecular weight (MW) that share a conserved alpha-crystallin domain. sHSPs oligomers exhibit chaperon-like activities by interacting with unfolded substrates, thereby preventing their aggregation and precipitation. Unlike most lactobacilli, which have single shsp genes, three different sHSP-encoding i.e. hsp1, hsp2, hsp3, were previously identified in the probiotic Lactobacillus plantarum WCFS1. Early studies, including characterization...
Bacterial strains of the Lactobacillaceae family are widely used as probiotics for their multifaceted potential beneficial properties. However, no official recommendations clinical use exist since, in many cases, oral administrations these bacteria displayed limited effects human. Additional research is thus needed to improve efficiency existing with strong potential. In this context, we assess vitro nine polyphenols stimulate biofilm formation by lactobacilli, a feature enhancing...
Abstract Background Biogenic amines are molecules with allergenic properties. They found in fermented products and synthesized by lactic acid bacteria through the decarboxylation of amino acids present food matrix. The concentration biogenic foodstuffs is influenced many environmental factors, particular, amine accumulation depends on quantity available precursors. Enological practices which lead to an enrichment nitrogen compounds therefore favor production wine. Free only known precursors...
Aim: To investigate how the survival of Listeria monocytogenes on parsley leaves may affect its ability to sustain process-related harsh conditions and virulence. Methods Results: Parsley seedlings were spot inoculated with stationary phase cells L. EGD-e incubated for 15 days. Each day, bacterial harvested enumerated, their survive acetic acid challenge (90 min, pH 4·0), colonize abiotic surfaces grow as biofilms was assessed. After a 3-log decrease over first 48 h, population stabilized...