A5045823220- Histone Deacetylase Inhibitors Research
- Peptidase Inhibition and Analysis
- Protease and Inhibitor Mechanisms
- Zebrafish Biomedical Research Applications
- Immune Cell Function and Interaction
- Biochemical Analysis and Sensing Techniques
- Receptor Mechanisms and Signaling
- melanin and skin pigmentation
- T-cell and B-cell Immunology
- Polymer Surface Interaction Studies
- Epigenetics and DNA Methylation
- Neuropeptides and Animal Physiology
- Nitric Oxide and Endothelin Effects
- Virus-based gene therapy research
- CAR-T cell therapy research
- Cell Adhesion Molecules Research
- Probiotics and Fermented Foods
- Dendrimers and Hyperbranched Polymers
- Bacterial biofilms and quorum sensing
- RNA Interference and Gene Delivery
- Bone Tissue Engineering Materials
- Phagocytosis and Immune Regulation
- Ear and Head Tumors
- Estrogen and related hormone effects
- Physiological and biochemical adaptations
Centre de recherche Translationnelle en Médecine moléculaire
2015-2021
CHU Dijon Bourgogne
2021
Université de Bourgogne
2007-2020
Inserm
2002-2018
Université Bourgogne Franche-Comté
2018
La Ligue Contre le Cancer
2014-2015
Sorbonne Paris Cité
2011
Centre National de la Recherche Scientifique
2000-2011
Délégation Paris 5
2011
Institut Cochin
2011
We report the development of new bioactive coatings biomaterials based on alternate deposition oppositely charged polyelectrolytes. selected polylysine (PLL) and poly(glutamic acid) (PGA) for polyelectrolytes murine melanoma cells as a biological test model system. These respond specifically to small peptide hormone, α-melanocortin, which is potent stimulator melanogenesis. show that synthetic α-melanocortin derivative, covalently coupled PLL forming outer layer multilayer film remains...
Abstract The aim of this study was to develop new biocompatible coatings for bone implants by the alternating deposition oppositely charged polyelectrolytes. Polyelectrolyte films were built up with different terminating layers on which SaOS‐2 osteoblast‐like cells and human periodontal ligament (PDL) grown. layer made one following polyelectrolytes: poly(ethylene imine) (PEI), poly(sodium 4‐styrenesulfonate) (PSS), poly(allylamine hydrochloride) (PAH), poly( L ‐glutamic acid) (PGA), or...
Abstract Upregulation of the matrix metalloproteinase (MMP)–9 plays a central role in tumor progression and metastasis by stimulating cell migration, invasion, angiogenesis. To gain insights into MMP-9 expression, we investigated its epigenetic control reversible model cancer that is initiated infection with intracellular Theileria parasites. Gene induction parasite was associated trimethylation histone H3K4 (H3K4me3) at promoter. Notably, found methyltransferase SMYD3 only upregulated upon...
Abstract Myelin basic protein (MBP)‐specific T cell lines and clones have been established from rats of the major histocompatibility complex (MHC)‐compatible Lewis BS strains. All are MHC class II restricted share CD 4 phenotype. The cells proliferate specifically in response to either a peptide representing amino acids #68–88 guinea pig MBP, residues #47–67 or an unidentified myelin antigen which is distinct MBP. specific for MBP express same receptor (TcR) variable (V) β chain element,...
Zebrafish were proposed as an alternative to mammalian models assess the efficacy and toxicity of antileukemic drugs. Due limited number transgenic zebrafish leukemia models, we explored human leukemic cell xenograft in embryos. Human lines blast cells sorted from patients with acute myelogenous injected 48 hours post-fertilization remained circulation embryos for several days without affecting their development. Imatinib oxaphorines did not demonstrate any on normal decreased burden animals...
The predominant form of life for microorganisms in their natural habitats is the biofilm mode growth. adherence and colonization probiotic bacteria are considered as essential factors immunoregulatory function host. Here, we show that Lactobacillus casei ATCC334 adheres to colonizes gut zebrafish larvae. abundance pro-inflammatory cytokines recruitment macrophages were low when inflammation was induced probiotic-fed animals, suggesting these have anti-inflammatory properties. We treated...
Autophagy is a lysosomal degradation process that contributes to host immunity by eliminating invasive pathogens and the modulating inflammatory response. Several infectious immune disorders are associated with autophagy defects, suggesting stimulation of in these diseases should be beneficial. Here, we show resveratrol able boost xenophagy, selective form target bacteria. We demonstrated promotes vitro autophagy-dependent clearance intracellular bacteria intestinal epithelial cells...
Journal Article In vivo primed mouse T cells selectively express cell-specific serine proteinase-1 and the proteinase-like molecules granzyme B C Get access Klaus Ebnet, Ebnet Search for other works by this author on: Oxford Academic PubMed Google Scholar Johanna Chluba-de Tapia, Tapia Ursula Hurtenbach, Hurtenbach 1Preclinical ResearchSandoz AG, CH-4002 Basel, Switzerland Michael D. Kramer, Kramer 2UniversitâtsklinikIm Neuenheimer Feld, D-6900 Heidelberg, Germany Markus M. Simon...
Abstract The aim of this study was to evaluate polyelectrolyte multilayer films as interfaces for implants. Polyelectrolyte multilayers were built up with different terminating layers by alternate deposition oppositely charged polyelectrolytes on which chondrosarcoma (HCS-2/8) cells grown in the presence serum. Films formed an increasing number investigated. layer made one following polyelectrolytes: poly-sodium-4-styrenesulfonate (PSS), poly-L-glutamic acid (PGA), poly-allylamine...
An analogue of human melanin‐concentrating hormone (MCH) suitable for radioiodination was designed in which Tyr 13 replaced by Phe and Val 19 Tyr. The resulting monoiodinated [ 125 I][Phe ,Tyr ]‐MCH radioligand biologically active led to the discovery high‐affinity binding sites on mouse B16‐F1, G4F G4F‐7 melanoma cells. Saturation analysis with cells revealed 1090 MCH receptors per cell a K D 1.18 × 10 −10 mol/l. Receptors were also found rat PC12 phaeochromocytoma cells, RE COS‐7...
To investigate the effect of nitric oxide on tumor development, we established a rat xenograft model in zebrafish embryos. The injected cells formed masses which production could be detected by use cell-permeant DAF-FM-DA (diaminofluorophore 4-amino-5-methylamino-2'-7'-difluorofluorescein diacetate) and DAR-4M-AM (diaminorhodamine-4M). This method revealed that co-localized with 46% In 85% these embryos, tumors were vascularized blood vessels observed day 4 post injection. Furthermore,...
The two mouse melanoma cell lines B16‐F1 and B16‐G4F retain their melanogenic capacity when cultured in vitro. Melanotropic peptides such as α‐melanocyte‐stimulating hormone (α‐MSH) induce formation release of melanin pigment cells. In contrast, cells do not respond to α‐MSH. Using receptor‐binding analysis photoaffinity crosslinking we demonstrate that the lack response α‐MSH is due absence functional MSH receptors from surface. Northern blot receptor mRNA revealed expressed These represent...
Abstract Up to now, most of the studies addressing critical roles played by protrusive and contractile cell‐matrix contacts in cell adhesion, guidance, migration, matrix assembly, activation signaling molecules have been performed on two‐dimensional surfaces. Here, we analysed organization chondrosarcoma a new three‐dimensional environment made titanium beads. Surface charges were modified deposition polyelectrolyte multilayer films built up alternated polycations poly‐(L‐lysine) or...