Uncovering the full potential of gas-fermenting Clostridia, attractive autotrophic bacteria capable using synthesis gases (CO–CO2–H2) to produce a range chemicals and fuels, for industrial applications relies on having efficient molecular tools genetic modifications. Although CRISPR-Cas9-mediated genome editing system has been developed in its use is limited owing low GC content (approx. 30%) these anaerobes. Therefore, effector protein Cas12a, which recognizes T-rich instead G-rich...

In this study, we used pan RNA-seq analysis to reveal the ubiquitous existence of both 5' and 3' end small RNAs (5' sRNAs). sRNAs alone can be annotate nuclear non-coding mitochondrial genes at 1-bp resolution identify new steady RNAs, which are usually transcribed from functional genes. Then, provided a simple cost effective way for annotation identification particularly long (lncRNAs). Using sRNAs, human was corrected novel ncRNA named RNA 1 (ncMT1) reported first time in study. We also...

Curcumin is the main secondary metabolite of Curcuma longa and other spp, has been reported to have some potential in preventing treating physiological disorders. This study investigated effect curcumin inhibiting high-fat diet streptozotocin (STZ)-induced hyperglycemia hyperlipidemia rats. Twenty-six male Sprague-Dawley (SD) rats (170-190 g) were randomly divided into a standard food pellet group (Control group), (HF + STZ combined with Cur group). Compared HF group, exhibited significantly...

To determine the anti-heat stress and antioxidant effects of genistein underlying mechanisms, lipofuscin, reactive oxygen species (ROS), survival under were first detected in Caenorhabditis elegans (C. elegans); then localization quantification fluorescent protein was determined by detecting fluorescently labeled mutant strain; addition, aging-related mRNAs applying real-time quantitative PCR C. elegans. The results indicate that substantially extended lifespan oxidative heat conditions;...

This study was carried out to understand the effects of zinc deficiency in rats aged 0∼2 months on learning and memory, brain-derived neurotrophic factor (BDNF) gene methylation status hippocampus.The lactating mother were randomly divided into three groups (n = 12): zinc-adequate group (ZA: 30 mg/kg diet), zinc-deprived (ZD: 1 a pair-fed (PF: which those ZD group. After weaning (on day 23), offspring fed same diets as their mothers. 37 days, concentrations plasma hippocampus measured,...

To enhance the bioavailability and antihypertensive effect of anti-depressant drug citalopram hydrobromide (CTH) we developed a sustained-release transdermal delivery system containing CTH. A diffusion meter was first used to determine optimal formulation CTH (TDDS). Then, based on determined formulation, patch prepared; its physical characteristics, including quality, stickiness, appearance, were evaluated, pharmacokinetics irritation skin evaluated by applying it rabbits rats. The TDDS...

Abstract Somatostatin‐14 [somatotropin release‐inhibiting factor (SRIF)] reduces hippocampal epileptiform activity but the contribution of its specific receptors (sst 1−5 ) is poorly understood. We have focused on role sst 1 and 2 in mediating SRIF modulation epilepsy using slices wild‐type (WT) or knockout (KO) mice. Recordings discharge induced by Mg 2+ ‐free medium with 4‐aminopyridine were performed from CA3 region before after application compounds. In WT mice, agonist CH‐275 reduce...

We aim to determine the significant effect of TPEN, a Zn(2+) chelator, in mediating pathophysiological cascade neuron death/apoptosis induced by hypoxia/ischemia.We conducted both vivo and vitro experiments this study. PC12 cells were used establish hypoxia/ischemia model applying oxygen-glucose deprivation (OGD). SHR-SP rats an acute ischemic electrocoagulating middle cerebral artery occlusion. The TPEN on was evaluated. In addition, relative biomarks excitotoxicity, oxidative stress,...

ABSTRACT The master regulator CcpA (catabolite control protein A) manages a large and complex regulatory network that is essential for cellular physiology metabolism in Gram-positive bacteria. Although can affect the expression of target genes by binding to cis -acting catabolite-responsive element ( cre ), whether how regulated remain poorly explored. Here, we report novel dual- motif employed Clostridium acetobutylicum , typical solventogenic species, autoregulation. Two sites are involved...

Metformin, a first-line drug for type-2 diabetes, plays potentially protective role in preventing Alzheimer's disease (AD), but its underlying mechanism is unclear. In this study, Aβ25-35 -treated SH-SY5Y cells were used as cell model of AD to investigate the neuroprotective effect metformin, well mechanisms. We found that metformin decreased apoptosis rate and death, ratio Bcl-2/Bax, expression NR2A NR2B, increased LC3 cells. Metformin also reduced intracellular extracellular Glu...

Abstract Background Berberine (BBR) is a commonly used anti-intestinal inflammation drug, and its anti-cancer activity has been found recently. BBR can intervene control malignant colorectal cancer (CRC) through intestinal microbes, but the direct molecular target related mechanism are unclear. This study aimed to identify of dissect mechanisms against occurrence development CRC from perspective microorganisms. Results Here, we that inhibits growth several CRC-driving bacteria, especially...

Memory performance, brain excitatory amino acid and acetylcholinesterase activity of chronically aluminum (Al) exposed mice in response to soy isoflavones (SI) treatment was investigated the study. Forty eight were allotted randomly into a control group, an Al group (100 mg/kg Al) treated with SI + 60 SI) for days. Chronic exposure significantly impaired long memory performance as assessed using passive avoidance task test (χ(2) analysis, p < 0.05). Interestingly, markedly improved score...

To investigate the effect of maternal zinc deficiency on learning and memory in offspring changes DNA methylation patterns.Pregnant rats were divided into adequate (ZA), deficient (ZD), paired fed (PF) groups. Serum contents AKP activity mother at P21 (end lactation) P60 (weaned, adult) detected. Cognitive ability determined by Morris water maze. The expression proteins including DNMT3a, DNMT1, GADD45β, MeCP2 BDNF hippocampus detected Western-blot. status promoter region was MS-qPCR.Compared...

The autotrophic acetogen Clostridium ljungdahlii has emerged as a major candidate in the biological conversion of one-carbon gases (CO2/CO) to bulk chemicals and fuels. Nevertheless, regulatory pathways downstream metabolic changes responsible for product formation distribution this bacterium remain minimally explored. Protein lysine acetylation (PLA), prevalent posttranslational modification, controls numerous crucial cellular functions. Herein, we revealed novel cross-regulatory mechanism...

In this study, we investigated the protective effects of genistein against SH‐SY5Y cell damage induced by β‐amyloid 25–35 peptide (Aβ ) and underlying mechanisms. Aβ‐induced neuronal death, apoptosis, glutamate receptor subunit expression, Ca 2+ ion concentration, amino acid transmitter apoptosis‐related factor expression were evaluated to determine on death apoptosis. The results showed that increased survival cells decreased level apoptosis Aβ . addition, reversed ‐induced changes in...

Protein lysine acetylation, a prevalent posttranslational modification, regulates numerous crucial biological processes in cells. Nevertheless, how acetylation interacts with other types of regulation to coordinate metabolism remains largely unknown owing the complexity process. Here, using representative gas-fermenting bacterium, Clostridium ljungdahlii, we revealed novel regulatory mechanism that employs both and transcriptional systems interactively control CO2 fixation, key process for...

Abstract The present study elucidates the possible protective effects of curcumin on β‐cells damaged by oxidative stress and its significance in controlling diabetes mellitus vitro experiments. Pancreatic islet (RIN‐m5F) cells were treated with 25 mmol/L alloxan (AXN) to induce cell damage observed. results showed that significantly promoted cellular activity AXN‐treated RIN‐m5F cells, decreased ratio apoptosis, downregulated level malondialdehyde, upregulated levels superoxide dismutase...