A5060918959- Proteoglycans and glycosaminoglycans research
- Glycosylation and Glycoproteins Research
- Cancer, Hypoxia, and Metabolism
- Microstructure and mechanical properties
- Cell death mechanisms and regulation
- interferon and immune responses
- Carbohydrate Chemistry and Synthesis
- Fibroblast Growth Factor Research
- Metal and Thin Film Mechanics
- Ion-surface interactions and analysis
- High Entropy Alloys Studies
- Connective tissue disorders research
- Synthesis and biological activity
- High-Temperature Coating Behaviors
- Platelet Disorders and Treatments
- Quinazolinone synthesis and applications
- Algebraic Geometry and Number Theory
- Advanced Algebra and Geometry
- Synthesis and Characterization of Heterocyclic Compounds
- Ubiquitin and proteasome pathways
- Fusion materials and technologies
- Alzheimer's disease research and treatments
- High-Velocity Impact and Material Behavior
- Nuclear Materials and Properties
- Nanoplatforms for cancer theranostics
NewGen Therapeutics (United States)
2024-2025
Chinese Academy of Sciences
2021-2025
Aerospace Information Research Institute
2025
North China Institute of Aerospace Engineering
2024-2025
Affiliated Hospital of Nantong University
2024
Nantong University
2024
Xi'an Jiaotong University
2011-2024
Nanjing Medical University
2022-2024
Jiangsu Province Hospital
2022-2024
University of North Carolina at Chapel Hill
2017-2024
Abstract Combinations of high strength and ductility are hard to attain in metals. Exceptions include materials exhibiting twinning-induced plasticity. To understand how the strength-ductility trade-off can be defeated, we apply situ , aberration-corrected scanning, transmission electron microscopy examine deformation mechanisms medium-entropy alloy CrCoNi that exhibits one highest combinations strength, toughness on record. Ab initio modelling suggests it has negative stacking-fault energy...
Abstract Due to its high diffusivity, hydrogen is often considered a weak inhibitor or even promoter of dislocation movements in metals and alloys. By quantitative mechanical tests an environmental transmission electron microscope, here we demonstrate that after exposing aluminium hydrogen, mobile dislocations can lose mobility, with activating stress more than doubled. On degassing, the locked be reactivated under cyclic loading move stick-slip manner. However, relocking thereafter requires...
The sulfation at the 3-OH position of glucosamine is an important modification in forming structural domains for heparan sulfate to enable its biological functions. Seven 3-O-sulfotransferase isoforms human genome are involved biosynthesis 3-O-sulfated sulfate. As a rare present sulfate, availability oligosaccharides very limited. Here, we report use chemoenzymatic synthetic approach synthesize six oligosaccharides, including three hexasaccharides and octasaccharides. synthesis was achieved...
Abstract Prion‐like transcellular spreading of tau in Alzheimer's Disease (AD) is mediated by binding to cell surface heparan sulfate (HS). However, the structural determinants for tau–HS interaction are not well understood. Microarray and SPR assays structurally defined HS oligosaccharides show that a rare 3‐ O ‐sulfation (3‐ ‐S) significantly enhances binding. In Hs3st1 −/− (HS ‐sulfotransferase‐1 knockout) cells, reduced ‐S levels diminished both internalization tau. culture, addition...
Abstract The selenium-containing enzyme GPX4 moonlights as a central regulator of ferroptosis, an iron-dependent, nonapoptotic form regulated cell death caused by lipid peroxidation. Yet, little is known about the mechanisms underlying regulation its post-transcriptional modifications. Here, we identify tripartite motif-containing protein TRIM26 E3 ubiquitin ligase GPX4. directly interacts with through Ring domain and catalyzes ubiquitination at K107 K117, which promotes switch in...
Abstract Background Abnormal activation of FAK is associated with tumor development and metastasis. Through interactions other intracellular signalling molecules, influences cytoskeletal remodelling, modulation adhesion signalling, transcription factors, promoting migration invasion cells. However, the exact mechanism that regulates these processes remains unresolved. Herein, our findings indicate S-palmitoylation crucial for both its membrane localization activation. Methods The...
In situ transmission electron microscope compression testing of submicron Al pillars shows two sample size regimes with contrasting behavior underlying the large strain bursts. For small pillars, bursts originate from explosive and highly correlated dislocation generation, characterized by very high collapse stresses nearly dislocation-free post-collapse microstructure. larger result reconstruction jammed configurations, featuring relative low stress levels retention network after
Coherent twin boundaries (CTBs) are internal interfaces that can play a key role in markedly enhancing the strength of metallic materials while preserving their ductility. They known to accommodate plastic deformation primarily through migration, experimental evidence documenting large-scale sliding CTBs facilitate has thus far not been reported. We show here CTB is possible whenever loading orientation enables Schmid factors leading and trailing partial dislocations be comparable each...
Helium implantation can cause the formation of a helium gas-bubble superlattice in crystalline materials. Experimental studies mechanical response material hosting such are lacking, especially at microstructural level. By employing novel, high-throughput, high-precision nanoscale technique, combination with in-situ transmission electron microscopy nanomechanical testing, we find that helium-bubble structure copper is disordered or maintained depending on whether material's plasticity occurs...
HS3ST1 is a genetic risk gene associated with Alzheimer’s disease (AD) and overexpressed in patients, but how it contributes to the progression unknown. We report analysis of brain heparan sulfate (HS) from AD other tauopathies using LC-MS/MS method. A specific 3- O -sulfated HS displayed sevenfold increase group ( n = 14, P < 0.0005). Analysis modified by recombinant sulfotransferases knockout mice revealed that made -sulfotransferase isoform 1 (3-OST-1), which encoded gene. synthetic...
To be successfully implemented, an efficient conversion, affordable operation and high values of CO2 -derived products by electrochemical conversion are yet to addressed. Inspired the natural CaO-CaCO3 cycle, we herein introduce CaO into electrolysis SnO2 in molten CaCl2 -NaCl establish situ capture . In anodic from graphite anode added generates CaCO3 The consequent co-electrolysis confines Sn carbon nanotube (Sn@CNT) cathode increases current efficiency O2 evolution 71.9 %. intermediated...
Heparan sulfate 6-endosulfatases (SULFs) remove 6-O-sulfo groups from heparan polysaccharide chains. SULFs modify the functions of and contribute to development cancers, organ endothelial inflammatory responses. However, direct measurement activity human mouse plasma is not currently possible. Here, we report a liquid chromatography coupled with tandem mass spectrometry (LS-MS/MS) assay measure SULFs. The method uses structurally homogeneous dodecasaccharide (12-mer) in which glucuronic...
ABSTRACT Stabilin‐2 is the primary scavenger for hyaluronan (HA) and binds to over two dozen other ligands including chondroitin sulfates, heparin, oxidized/acetylated LDL, etc. Although rat liver sinusoidal endothelial cells are preferred cell lines animal physiological studies of Stab2/HARE, recombinant protein has never been characterized. Since Stab2/HARE a high degree homology mouse which cloned, our hypothesis that receptor identical very similar human receptor. Rat was cloned...
Significance Producing strong and defect-free materials is an important objective in developing many new materials. Thermal treatments aimed at defect elimination often lead to undesirable levels of strength other properties. Although monotonic loading can reduce or even eliminate dislocations submicroscale single crystals, such “mechanical healing” causes severe plastic deformation significant shape changes. Inspired by observing easier pullout a partly buried object after shaking it first,...
The 3-O-sulfated glucosamine in heparan sulfate (HS) is a low-abundance structural component, but it key saccharide unit for the biological activities of HS. A method to determine level HS lacking. Here, we describe LC–MS/MS based analyze motifs. We determined levels motifs from pharmaceutical heparin manufactured bovine, porcine, and ovine. discovered that chains carrying 3-O-sulfation enoxaparin, an FDA-approved low-molecular weight heparin, displayed slower clearance rate than...
Through in situ scanning electron microscope microcompression tests, we demonstrated that the strain-rate sensitivity of body-centered cubic single crystal iron pillars will be reduced by one order when pillar size was from 1000 to about 200 nm. In addition, size-strengthening exponent exhibited obvious dependence. We propose observed behavior is a result high stresses required induce curvature bowout dislocation arms at small sample or grain sizes, which overwhelms lattice friction stress...
Abstract Recent studies suggest that Forkhead box D1 (FOXD1) plays an indispensable role in maintaining the mesenchymal (MES) properties of glioblastoma (GBM) stem cells (GSCs). Thus, understanding mechanisms control FOXD1 protein expression is critical for guiding GBM treatment, particularly patients with therapy-resistant MES subtypes. In this study, we identify ubiquitin-specific peptidase 21 (USP21) as a deubiquitinase GSCs. We find USP21 directly interacts and stabilizes by reverting...