Deoxynivalenol (DON) is a common environmental pollutant that poses serious health risk to humans worldwide. This study was aim explore whether gut microbiota involved in DON-induced intestinal toxicity as well reveal effect of probiotics derived from protecting barrier and elucidate mechanism. We found DON caused disturbed microbiota, particularly Lactobacillus murinus (L. murinus) deficiency. enhanced M1 macrophage polarization decreased tight junction protein expression. Microbiota...

Diarrheic disease is a common intestinal health problem worldwide, causing great suffering to humans and animals. Precise manipulation strategies based on probiotics combat diarrheic diseases have not been fully developed.

Abstract Lactobacillus species have excellent abilities to reduce intestinal inflammation and enhance gut barrier function. This study elucidated the potential mechanisms through which mitigates lipopolysaccharide (LPS)-induced injury from perspective of macrophage–intestinal epithelial cell interactions. intervention improved histopathological score; elevated ZO-1 Occludin protein production; reduced CD16 + numbers; diminished IL-1β, IL-6, TNF-α levels; decreased inducible nitric oxide...

A Gram-stain-negative, rod-shaped bacterium, designated strain Y9(T), was isolated from a soil sample collected in Ningxia Province China and characterized to determine its taxonomic position. Strain Y9(T) contained Q-8 as the predominant ubiquinone. Major fatty acid components were summed feature 3 (C(16:1)ω7c and/or iso-C(15:0) 2-OH) C(16:0). The major polar lipids phosphatidylethanolamine, phosphatidylglycerol diphosphatidylglycerol. G+C content of genomic DNA 68.7 mol%. phylogenetic tree...

A gram-positive, acid-fast and rapidly growing rod, designated F4(T), was isolated from a soil sample of Haikou in China. The isolate shared 98.2 % 16S rRNA gene sequence similarity with Mycobacterium monacense B9-21-178(T), 96.2 hsp65 M. FI-05352 79.6 16S-23S internal transcribed spacer B9-21-178(T). DNA-DNA relatedness between the DSM 44395(T) 43.5 %. morphological analysis physiological tests also showed that differed any strain reported to date. mycolic acid profile cellular fatty...

A bacterial isolate, designated strain D5(T), was isolated from a soil sample collected the Inner Mongolia Autonomous Region, China, and subjected to taxonomic investigation using polyphasic approach. Strain D5(T) aerobic, Gram-stain-negative, rod-shaped motile. fell within evolutionary radius of genus Massilia in phylogenetic tree based on 16S rRNA gene sequences most closely related plicata 76(T) with 97.3% sequence similarity. The predominant quinone Q-8. major polar lipids were...

A bacterium, designated XC21-2 T , was isolated from a saline-alkaline soil sample China. Cells were Gram-stain-negative, rod-shaped and motile grew optimally at 35–37 °C, pH 6.0–7.0 in the presence of 0.5 % (w/v) NaCl. Growth occurred range 5.5–9.0 up to 4 The major cellular fatty acids iso-C 15 : 0 16 17 1 ω9 c . polar lipids phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol an uncharacterized amino-group-containing lipid. quinone ubiquinone 8 (Q-8) G+C content genomic...

Glutathione S-transferase CL 3 subunits purified from 1-day-old-chick livers were digested with Achromobacter proteinase I and the resulting fragments isolated for amino acid sequence analysis. An oligonucleotide probe was constructed accordingly cDNA library screening. A clone of 1342 bases, pGCL301, encoding a protein 26209 Da sequenced. Including conservative substitutions, this has 75-79% similarity to other Alpha family glutathione S-transferases. The coding pGCL301 inserted into...

In this study, by using of near-field scanning optical microscopy (NSOM)/immune-labeling quantum dot (QD)-based dual-color imaging system, we achieved the direct visualization nanoscale profiles for distribution and organization CD4 CD25 molecules in T cells. A novel interesting finding was that though clustering as nanodomains were observed on surface CD4(+)CD25(high) regulatory cells, these not co-localized with nanodomains. This result presented formation cells associated response cell...

Abstract Oxidative stress is an important contributor to gastrointestinal diseases in multiple ways. Taurodeoxycholic acid (TDCA) a metabolite of bile acids and has anti-inflammatory protective effects on the intestinal tract. However, whether TDCA can alleviate oxidative intestine still unclear. Here, we investigated diquat-induced jejunum its mechanism. The results revealed that increased concentrations antioxidant enzymes serum, jejunal tissue epithelial cells mice, as did expression...

The goal of Target Sound Extraction (TSE) is to isolate sound signals interest from a mixture overlapping sounds. In learning-based TSE approaches, the cues, such as embedding, which provide information about characteristics, are used auxiliary features for Deep Neural Networks (DNNs) extract target signals. However, in presence strong interference, extraction performance reduced when model relies solely on embedding. To this end, study proposes an efficient Target-Direction (TDSE) system by...

Audio Tagging (AT) is a critical technique in smart home applications, enabling continuous monitoring of specific sound events for subsequent surveillance systems. Although Deep Neural Networks (DNNs) can provide promising tagging results, the performance degrades significantly adverse acoustic environments. In addition, if AT system both and location it greatly enhance system’s ability to capture events. To this end, multichannel audio based on Convolutional Network (CNN) proposed...

NK cells play the important role in adoptive cell therapy. As we know, NK-92 cell, a line, has been used for preparation of therapy product clinical trials some laboratory and had showed potent effect cancer Except NK-92, another line named NKG was established by Dr Tian Zhigang University Science Technology China. derived from male patient with non-Hodgkin's lymphoma. It isolated peripheral blood samples cultivated through long-lasting period medium containing horse fatal bovine serum IL-2....

Objective To detect the protection induced by HPV-58 L2 11-200 AA in animal, and analyze relationship between antibody or neutralizing titers generated immunizmg agent. Methods The peptide of was expressed E. coli mice were immunized with after purification adsorption aluminum adjuvant. provided different immunizing doses detected mouse model against challenge pseud-ovirions human papiilomavirus types 58. total antibodies serum tested ELISA neutralization assay pseudovirus, respectively....

Objective To study the relationships between differences in tumorigenicity and immunogenetic backgrounds of nude mice. Methods According to Chinese Pharmacopoeia, positive negative groups were set up both Laboratory A B with ten mice each group. Organ tissues collected for clinicopathological analysis. Blood samples detected using flow cytometry. DNA was extracted analyzed 23 STR markers. Results The group invalid (7/10 tumor formation). The two laboratories showed no...