A5102757054- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Malaria Research and Control
- Viral Infections and Outbreaks Research
- Biosensors and Analytical Detection
- SARS-CoV-2 and COVID-19 Research
- Virology and Viral Diseases
- SARS-CoV-2 detection and testing
- COVID-19 epidemiological studies
- Vector-borne infectious diseases
- Influenza Virus Research Studies
- Bacillus and Francisella bacterial research
- Respiratory viral infections research
- Vector-Borne Animal Diseases
- Vibrio bacteria research studies
- Animal Disease Management and Epidemiology
- HIV Research and Treatment
- Dengue and Mosquito Control Research
- Advanced biosensing and bioanalysis techniques
- Bacteriophages and microbial interactions
- Poxvirus research and outbreaks
- Aquaculture disease management and microbiota
- Cytomegalovirus and herpesvirus research
- RNA Interference and Gene Delivery
- Long-Term Effects of COVID-19
Defence Research and Development Establishment
2013-2024
Defence Research and Development Organisation
2023
Ministry of Defence
2019
Institut Pasteur
2012
Centre National de la Recherche Scientifique
2012
Research & Development Establishment (Engrs.)
2009
Baba Raghav Das Medical College
2006
ABSTRACT The development and validation of a one-step, real-time, quantitative dengue virus serotype-specific reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay targeting the 3′ noncoding region for rapid detection differentiation serotypes are reported. RT-LAMP is very simple rapid, wherein can be obtained in 30 min under conditions at 63°C by employing set four primer mixtures through real-time monitoring an inexpensive turbidimeter. evaluation use clinical...
The standardization and validation of a one-step, single-tube, accelerated, quantitative reverse transcription (RT) loop-mediated isothermal amplification (RT-LAMP) assay targeting the E1 gene for rapid real-time detection Chikungunya virus (CHIKV) are reported. A linear relationship between amount template time positivity value over range 2 x 10(8) to 10(2) copies was obtained. feasibility CHIKV RT-LAMP clinical diagnosis validated with patient serum samples from an ongoing epidemic in...
Abstract Background Dengue virus type 1 (DENV-1) have been mostly circulating silently with dominant serotypes DENV-2 and DENV-3 in India. However recent times marked an increase DENV-1 circulation yearly outbreaks. Many studies not carried out on this type, leaving a lacunae pertaining to the genotypes, since its earliest report In present study, we sequenced CprM gene junction of 13 isolated from Delhi Gwalior (North India) between 2001–2007 one 1956 Vellore isolate as reference. For...
An epidemic of Chikungunya fever unprecedented magnitude occurred in many parts India early 2006 after an interval 33 years, and there has been a resurgence some South since June 2007. The article highlights clinical manifestations infection various molecular tests that were used for diagnoses virus infection. Of particular interest is the real-time loop-mediated isothermal amplification (RT LAMP) assay, which rapid cost-effective can be adopted at ill-equipped laboratories. Clinical...
Abstract Background Dengue virus infection has recently taken endemic proportion in India implicating all the four known dengue serotypes. There was a major outbreak northern including Delhi October- December, 2003 and again 2004. We have carried out detailed investigation of 2004 by Serosurveillance, RT-PCR, nested PCR, isolation genotyping. also report molecular epidemiological these outbreaks. Results The serological 162 suspected serum samples using an in-house dipstick ELISA revealed...
Abstract An outbreak of viral encephalitis occurred in Gorakhpur, India, from July through November 2005. The etiologic agent was confirmed to be Japanese virus by analyzing 326 acute-phase clinical specimens for virus-specific antibodies and RNA isolation. Phylogenetic analysis showed that these isolates belonged genogroup 3.
ABSTRACT The standardization and validation of a one-step, single-tube accelerated quantitative reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay is reported for rapid real-time detection Japanese encephalitis virus (JEV). RT-LAMP in this study very simple rapid; the can be obtained 30 min under conditions at 63°C by employing set six primers targeting E gene JEV. demonstrated exceptionally higher sensitivity compared to that RT-PCR, with limit 0.1 PFU....
Dengue (DEN) is an acute mosquito borne viral disease of mankind. Off late it has become important public health concern in Southeast Asia. Although, all the four known dengue virus serotypes (DEN-1 to 4) are reported from time time, recent past, DEN-2 emerged as predominant type, being causative agent several outbreaks fever (DF) and haemorrhagic (DHF) India. To elucidate true molecular epidemiology these viruses, we have sequenced C-prM gene junction (454 nucleotides) 11 viruses directly...
SUMMARY Dengue is an emerging arboviral disease and currently poses the greatest threat to human health. In recent decades, there has been a substantial increase in dengue outbreaks many parts of world including India. We performed in-depth investigation major outbreak Andhra Pradesh, southern India 2007 by serology, virus isolation, RT–PCR genotyping. The results revealed unusual emergence type 4 (DENV-4) along with prevailing DENV-3. Phylogenetic analysis based on complete envelope gene...
Abstract Background Dengue is emerging as a major public health concern in many parts of the world. The development one-step, single tube, rapid, and multiplex reverse transcription polymerase chain reaction (M-RT-PCR) for simultaneous detection typing dengue virus using serotype specific primers during acute phase illness reported. Results An optimal assay condition with zero background was established having no cross-reaction closely related members flavivirus (Japanese encephalitis, West...
Chikungunya has resurged in the form of unprecedented explosive epidemic 2006 after a long gap India affecting 1.39 million persons. The disease continued for next two consecutive years 59,535 and 64,548 persons during 2007 2008 respectively. outbreak being second largest among these three information regarding etiology mutations involved are useful further control measures. Among outbreaks Coastal Karnataka accounts 46,510 An in-depth investigation Karnataka, India, by serology, virus...
Abstract Background Yellow Fever virus (YFV) is an important arboviral pathogen in much of sub-Saharan Africa and the tropical Americas. It prototype member genus Flavivirus transmitted primarily by Aedes (Stegomyia) mosquitoes. The incidence human infections endemic areas has risen recent years. Prompt dependable identification YFV a critical component response to suspect cases. Results We developed one-step SYBR Green I-based real-time quantitative RT-PCR (qRT-PCR) assay targeting 5'NTR...
Dengue is an acute viral infection which presents as uneventful pyrexia to a fatal complication. This increasingly being recognized the world's major emerging tropical disease and important public health problem. article highlights clinical manifestations of virus various molecular tests that were used for its laboratory diagnosis.Serum samples from 713 suspected cases collected between August December 2007. The profiles 123 hospitalized patients analyzed. Serology, RT- PCR, isolation...