A5034441382- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Malaria Research and Control
- HIV Research and Treatment
- Viral Infectious Diseases and Gene Expression in Insects
- Vector-Borne Animal Diseases
- Protein purification and stability
- Transgenic Plants and Applications
- Vibrio bacteria research studies
- Poxvirus research and outbreaks
- Fire effects on ecosystems
- Dengue and Mosquito Control Research
- Phytoplasmas and Hemiptera pathogens
- HIV/AIDS drug development and treatment
- Herpesvirus Infections and Treatments
- Virology and Viral Diseases
- Bacillus and Francisella bacterial research
- Vector-borne infectious diseases
Defence Research and Development Establishment
2011-2023
Research & Development Establishment (Engrs.)
2011-2014
Purpose: Dengue is one of the most serious mosquito-borne viral infections affecting tropical and subtropical countries in world. Since there no immunoprophylactic or specific antiviral therapy available, timely rapid diagnosis plays a vital role patient management implementation control measures. This paper evaluates commercially available NS1 antigen capture ELISA vis-a-vis SD bioline test for early detection dengue virus. Materials Methods: To evaluate commercial kit test, total 91...
Abstract Background Dengue is emerging as a major public health concern in many parts of the world. The development one-step, single tube, rapid, and multiplex reverse transcription polymerase chain reaction (M-RT-PCR) for simultaneous detection typing dengue virus using serotype specific primers during acute phase illness reported. Results An optimal assay condition with zero background was established having no cross-reaction closely related members flavivirus (Japanese encephalitis, West...
Chikungunya virus has emerged as one of the most important global arboviral threats over last decade. Inspite large scale morbidity, with long lasting polyarthralgia, so far no licensed vaccine or antiviral is available. CHIKV nsP2 protease crucial for processing viral nonstructural polypeptide precursor to release enzymes required replication, thus making it a promising drug target. In this study, high cell density cultivation (HCDC) Escherichia coli in batch process was carried out produce...
Dengue fever, a mosquito-borne viral disease, has become major public health problem with marked expansion in recent decades. now hyperendemic India co-circulation of all the four serotypes. Herein, we report an unprecedented outbreak which occurred during August to October 2011 Odisha, eastern India. This is first large epidemic Odisha. Detailed serological and molecular investigation was carried out identify aetiology. Almost half samples were found be dengue antigen (NS1) positive....
Mpox (previously known as Monkeypox) has recently re-emerged, primarily through human-to-human transmission in non-endemic countries including India. Virus isolation is still considered the gold standard for diagnosis of viral infections. Here, qPCR positive skin lesion sample from a patient was inoculated Vero E6 cell monolayer. Characteristic cytopathic effect exhibiting typical rounding and detachment observed at passage-02. The virus confirmed by qPCR. replication kinetics isolate...
Crimean-Congo hemorrhagic fever (CCHF) is a re-emerging zoonotic viral disease prevalent in many parts of Asia, Europe and Africa. The causative agent, Orthonairovirus (CCHFV) transmitted through hard ticks. Tick vectors especially belonging to Hyalomma species serve as the reservoir amplifying host. vertebrate animals including sheep, goat bovine act short lasting bridge linking virus . CCHFV causes fatal humans. Humans are usually infected with either bite ticks or by close contact...
Abstract Crimean-Congo hemorrhagic fever (CCHF) virus, a highly pathogenic viral agent is responsible for severe fatal infections in many parts of the world. The early diagnosis CCHF infection important successful clinical management and epidemiological control. nucleoprotein (NP) CCHFV being conserved immunogenic used as diagnostic marker. In this study, we report rapid sensitive double antibody based antigen capture ELISA to detect virus (CCHFV). Highly specific polyclonal monoclonal...
Abstract Dengue is a public health problem of global significance for which there neither an effective antiviral therapy nor preventive vaccine. The envelope protein dengue virus the major antigen to elicit neutralizing antibody response and protective immunity in hosts. Optimization culture media was carried out enhanced production recombinant type 3 domain III (rDen EDIII) E. coli . Further, batch fed‐batch cultivation process were also developed optimized medium. After cultivation, dry...
Chikungunya fever, a re-emerging infection, is an arthropod-borne viral disease prevalent in different parts of the world, particularly Africa and South East Asia. virus envelope 2 protein involved binding to host receptors it contains specific epitopes that elicit neutralizing antibodies. A highly immunogenic, recombinant was produced by bioreactor Escherichia coli for development suitable diagnostic vaccine candidate. This refolded further purified achieve biologically active protein. The...
Dengue, a mosquito borne viral disease caused by dengue virus has emerged as major health problem during the last few decades. The envelope domain III (DIII) protein of is highly immunogenic and capable inducing neutralizing antibodies against wild-type virus. potential subunit vaccine candidate well diagnostic reagent for dengue. This report describes high yield production immunogenicity recombinant DIII proteins type 1 2. were produced in